LOCUS       sequence-392699-       10270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1921
FEATURES             Location/Qualifiers
     source          1..10270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3208..3796
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3261..3281)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3679..3690)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3868..4101
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4179..4300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4216..4235)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4270..4289
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4340..4475
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4495..4514)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4496..4514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4524..4541)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4524..4540)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4533..4555)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4682..5137
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4769..4788)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4979..5000
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5163..5267
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5268..5336,5337..6128)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5486..5505)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6299..6887
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6788..6807
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7041..7058
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7175..7196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7211..7216,7217..7234,7235..7241)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7249..7265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7254..7276
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7273..7289
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7273..7289
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7308..7328
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7310..7328
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7356..7582
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7583..7763
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7810..7935
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8428..8661
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8846..8890
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9121..9331
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9143..9339
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9190..9325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9371..9973
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9371..9390)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9867..9887
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10016..10133
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 gccatctcct ctgtctccca gtggggcagc acttcccctg ccgctgtcgc tggctccgcc
     1261 cccactaccc ctgccagcag ctgcagtggt acatgtgtcc ttccctgagg tgaccagtgc
     1321 cctcttggag tccctcaatc agcagcgtct gcagggccag ctctgcgatg tatctatcag
     1381 agtgcagggc cgggagttcc gggctcatcg ggctgtcctg gctgcctcct ccccttactt
     1441 ccatgatcag gtcctactca aaggcatgac ctccatctcg ctgcccagtg tcatggaccc
     1501 aggcgccttt gagactgtcc tagcctccgc ttacactggc cgcctcagca tggctgctgc
     1561 tgacattgtc aacttcctta cagtggggtc tgtgctccaa atgtggcaca ttgtggacaa
     1621 gtgcactgaa ctactccgag aaggccgggc ctcagctacc accaccatca ctactgctgc
     1681 agccacctct gtcactgtcc ctggtgctgg ggtgccatcc gggagtgggg gcactgtggc
     1741 ccctgctacc atgggctctg cgcgctccca tgcctccagc cgggccagtg agaatcaatc
     1801 tcccagcagc agcaactact tcagccccag ggagtccact gatttctcat cttcctccca
     1861 agaggcattt gcagcttctg cagtgggcag tggggagcgt cgaggaggtg gccctgtatt
     1921 cccagcccct gtcgttggca gtggaggggc cacatctgga aagctgctgc tggaggcaga
     1981 tgagctgtgc gatgatggtg gggatgggag gggggcagtg gttcctgggg ctgggctccg
     2041 gagacccacc tacacacccc ctagcatcat gccacagaaa cactgggtat acgtgaagcg
     2101 aggtggtaat tgcccagcgc caacacccct ggttccccaa gacccagatc tggaggagga
     2161 agaggaggag gaagatctgg tgttgacctg tgaggatgat gaagatgaag aactaggggg
     2221 tagctccagg gttccagtgg ggggagggcc tgaggctacc ctcagcataa gtgatgtccg
     2281 taccctgagt gagcccccag acaaggggga ggagcaggtc aacttctgtg agtcctccaa
     2341 tgactttggc ccatatgagg gtgggggtcc tgtggcaggt cttgatgact caggggggcc
     2401 aactccctct tcctatgccc cctcccaccc tcctcgaccg ctccttccct tggacatgca
     2461 gggcaaccag atcctggtct tcccgtcgtc gtcttcatcc tcatcctcac aggctcctgg
     2521 ccaaccacca gggaaccaag cagaacacgg ggcagtgacc gtggggggca cgtcggtggg
     2581 gagcctgggt gtgccgggta gcgttggtgg ggtccctgga gggactggca gtggggacgg
     2641 gaataagatc tttctgtgcc attgtgggaa ggccttctcc cacaagagca tgcgggaccg
     2701 gcacgtgaac atgcacctca atctgcggcc gtttgactgc cccgtgtgca acaaaaagtt
     2761 caagatgaag caccatctga ctgagcacat gaagacgcac acaggtctca agccctacga
     2821 gtgcggagtc tgcgccaaga agttcatgtg gcgagacagc ttcatgcgcc accgaggaca
     2881 ctgtgagcgc cggcaccgcc tgggcggggt cggggccgta cctgggcctg ggactcccac
     2941 ggggccatcc ttgccgtcca agagagagtc tcccggagtg ggcgggggca gcggcgacga
     3001 agcgagtgcg gccacgcccc cgtccagcag acgtgtctgg tccccaccca gagtccacaa
     3061 ggtggagatg ggcttcggtg gaggtggagg agcaaactag taatgaacta gtccgtaact
     3121 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgacg acgctatagt
     3181 tcaacgactc tcacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     3241 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3301 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3361 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3421 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3481 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3541 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3601 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3661 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3721 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3781 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     3841 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     3901 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     3961 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4021 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4081 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     4141 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     4201 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4261 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     4321 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4381 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4441 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     4501 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4561 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4621 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4681 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4741 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4801 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4861 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4921 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4981 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5041 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5101 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     5161 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5221 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5281 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5341 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5401 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5461 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5521 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5581 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5641 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5701 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5761 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5821 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5881 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5941 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6001 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6061 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6121 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6181 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6241 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6301 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6361 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6421 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6481 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6541 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6601 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6661 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6721 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6781 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6841 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6901 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6961 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7021 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7081 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7141 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7201 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7261 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     7321 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     7381 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     7441 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     7501 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     7561 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     7621 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7681 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7741 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7801 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7861 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     7921 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     7981 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     8041 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     8101 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     8161 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     8221 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     8281 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     8341 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     8401 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     8461 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8521 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8581 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8641 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8701 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8761 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8821 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     8881 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     8941 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     9001 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     9061 acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     9121 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
     9181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
     9361 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     9421 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     9481 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     9541 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     9601 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9661 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9721 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9781 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9841 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     9901 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     9961 cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
    10021 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
    10081 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
    10141 attacaggga cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg
    10201 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
    10261 agtgggaatt
//
