LOCUS       sequence-392982-        8311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of truncated HSF1 (DBD and HR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS
  TITLE     Heat shock transcription factors demonstrate a distinct mode of 
            interaction with mitotic chromosomes.
  JOURNAL   Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304.
  PUBMED    37114996
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304."
COMMENT     Sequence Label: H1DBD-Halo-HA
FEATURES             Location/Qualifiers
     source          1..8311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        89..300
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             313..581
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             1099..1119
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             1129..2019
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             2020..2043
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2047..2073
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2084..2656
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2251..2268)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2478..2497
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2665..3459
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2719..2738)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3329..3348
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3519..3539)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    3581..3702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3618..3637)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3672..3691
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4465..4499
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4990..5006)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4990..5006)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5003..5025)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5014..5030
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5038..5044,5045..5062,5063..5068))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5083..5104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5221..5238)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5392..5980)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5472..5491)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6151..6942,6943..7011))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6774..6793
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7012..7116)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7142..7597)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7279..7300)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7491..7510
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7724..7746
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7738..7755
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7739..7755
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   7885..7947
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ctgctctttg agcctgcaga cacctggggg gatacgggga aaaggcctcc acggccaagg
       61 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
      121 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
      181 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
      241 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
      301 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
      361 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
      421 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
      481 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
      541 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
      601 actatggatc tggccgtggg ccccggtgca gcggggccca gcaacgtccc ggccttccta
      661 accaagctgt ggaccctcgt gagcgacccg gacacagacg cgctcatctg ctggagcccg
      721 agtgggaaca gcttccacgt gtttgaccag ggccagtttg ccaaggaggt gctgcccaag
      781 tacttcaagc acaacaacat ggctagcttc gtgcggcagc tcaacatgta tggcttccga
      841 aaagtagtcc acattgagca gggtggcctg gtcaagcctg agagagatga caccgagttc
      901 cagcatcctt gtttcttgcg tggacaggaa cagctccttg agaacatcaa gaggaaagtg
      961 accagcgtgt ccaccctgaa gagtgaggac ataaaaatac gcggtggcgg tggctcgaag
     1021 agaaagatcc ctctgatgtt gagtgacagc aactcagcac actctgtgcc caagaattcc
     1081 tcgaggacta gtcctcgaga ggatctgtac tttcagagcg gaggctccat ggcagaaatc
     1141 ggtactggct ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac
     1201 gtcgatgttg gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc
     1261 tcctacgtgt ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca
     1321 gacctgatcg gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac
     1381 gtccgcttca tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt
     1441 cacgactggg gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa
     1501 ggtattgcat ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt
     1561 gcccgcgaga ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat
     1621 cagaacgttt ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc
     1681 gagatggacc attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc
     1741 ttcccaaacg agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa
     1801 tacatggact ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc
     1861 gttctgatcc caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct
     1921 gtggacatcg gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc
     1981 gagatcgcgc gctggctgtc gacgctcgag atttccggcg actacaaaga cgatgacgac
     2041 aagatgtacc catacgatgt tccagattac gcttaaggcc gcgcccctct ccctcccccc
     2101 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     2161 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     2221 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     2281 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     2341 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     2401 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     2461 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     2521 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     2581 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     2641 gaaaaacacg ataatacctc cggaatgatt gaacaagatg gattgcacgc aggttctccg
     2701 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     2761 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     2821 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccgcg
     2881 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     2941 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     3001 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     3061 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     3121 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     3181 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     3241 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     3301 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     3361 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     3421 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag tcgacaatca acctctggat
     3481 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3541 ggatacgctg ctttaatgcc tttgtatcat gcgttaacta aacttgttta ttgcagctta
     3601 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3661 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggaattgact
     3721 caaatgatgt caattagtct atcagaagct catctggtct cccttccggg ggacaagaca
     3781 tccctgttta atatttaaac agcagtgttc ccaaactggg ttcttatatc ccttgctctg
     3841 gtcaaccagg ttgcagggtt tcctgtcctc acaggaacga agtccctaaa gaaacagtgg
     3901 cagccaggtt tagccccgga attgactgga ttcctttttt agggcccatt ggtatggctt
     3961 tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga
     4021 aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg
     4081 atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg
     4141 ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct gatatctata
     4201 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata acaatataat
     4261 tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca
     4321 cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg
     4381 agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa
     4441 agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt
     4501 taatctagct gcatcaggat catatcgtcg ggtctttttt ccggctcagt catcgcccaa
     4561 gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc gaggttgaag
     4621 cggcatggaa agagtttgcc gaggatgact gctgctgcat tgacgttgag cgaaaacgca
     4681 cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg
     4741 ttcaggccac ctgggatacc agttcgtcgc ggcttttccg gacacagttc cggatggtca
     4801 gcccgaagcg catcagcaac ccgaacaata ccggcgacag ccggaactgc cgtgccggtg
     4861 tgcagattaa tgacagcggt gcggcgctgg gatattacgt cagcgaggac gggtatcctg
     4921 gctggatgcc gcagaaatgg acatggatac cccgtgagtt acccggcggg cgcgcttggc
     4981 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5041 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5101 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5161 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5221 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5281 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5341 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5401 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5461 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5521 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5581 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5641 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5701 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5761 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5821 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5881 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5941 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6001 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6061 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6121 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6181 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6241 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6301 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6361 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6421 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6481 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6541 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6601 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6661 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6721 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6781 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6841 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6901 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6961 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7021 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7081 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7141 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     7201 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     7261 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     7321 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     7381 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     7441 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     7501 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     7561 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     7621 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     7681 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     7741 aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt ttgaacccgt ggaggacggg
     7801 cagactcgcg gtgcaaatgt gttttacagc gtgatggagc agatgaagat gctcgacacg
     7861 ctgcagaaca cgcagctaga ttaaccctag aaagataatc atattgtgac gtacgttaaa
     7921 gataatcatg tgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta
     7981 ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca
     8041 acaaacaatt tatttatgtt tatttattta ttaaaaaaaa caaaaactca aaatttcttc
     8101 tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca gggatgtaat
     8161 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     8221 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     8281 cacgggatcg ctttcctctg aacgcttctc g
//
