LOCUS       sequence-393012-        9088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in
            insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9088)
  TITLE     Bacterial expression plasmids for production of recombinant 
            Na,K-ATPases of modern and ancestral birds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Grosbeak
FEATURES             Location/Qualifiers
     source          1..9088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(5216..5325)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        5349..5440
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5365..5382
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    8678..8812
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8715..8734)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8769..8788
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  8841..9006
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtacctt aggaggactt gatatcgaac ttgatgtcga agcggccctg
     4321 gaaacggtcc ttgtcagagt agatgatgtt ctggccgtaa gcacggcact cgacacgcac
     4381 ttccacgtcg taggtcaggt tggtgaactg cacagccacc agaggttgca ggtaacgggg
     4441 ctgcagcagc tttccgtagt aggggtagta ctgcaggccg aaaccagggt aaccgcccat
     4501 gccgtagtac tcgacggtgc cgatcttgtc agcgtcctct tcacgcttgg ccacgcagtg
     4561 cacggggatc aagttggggt tgtacttagc catcacttct ggaggcaggg actcgttgat
     4621 gggagcctcg ggcttgaaac cgatgatacg gttcagcttg accaggatgc aaggcttgcc
     4681 gtccttgtaa ccgtaggtgg ggtcgttgat accggagcag ttctcgagcc actcgcgctt
     4741 gaacttgcag actttcttct ggccctgagc gtcgttgtag ggaccacgac ctttgtactc
     4801 ggtaggggtg tcgccgcagt cctcgaacac gatgttctcg atctgctgct cgtcggagta
     4861 gtcgcgcagg aagttgtcca ggttacgcac gtagggctcg taggaacgag ggtcagaagc
     4921 ggtgaaagcg atctcggtct tttgcacctg aggcacttgg gtcagtccag gaggagccac
     4981 acggtcttgg tacttgggct cgaactcgga cacggtcagc agcatcactt ggatggtgcc
     5041 gatgaagata ccagccaggc aaccgtagaa gatcacgtag aacagcagga tcttgaacca
     5101 ggaaccaccg gtacgaccca gcagctcttt cttctcggag ttccagatga acttcttcca
     5161 gttgccgtcg ccgtctttag ccttgccacg agccatgatc aagtcttcgt cgagtgattg
     5221 taaataaaat gtaatttaca gtatagtatt ttaattaata tacaaatgat ttgataataa
     5281 ttcttattta actataatat attgtgttgg gttgaattaa aggtccgtat actccggaat
     5341 attaatagat catggagata attaaaatga taaccatctc gcaaataaat aagtatttta
     5401 ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt attcataccg
     5461 tcccaccatc gggcgcggat ccatgggcaa aggtgctggt cgtgataagt acgagcccac
     5521 cgctacctct gagcacggtg gaaagaagaa aggcaagaaa gaacgcgaca tggacgagct
     5581 gaagaaagag gtggtcatgg acgaccacaa gctgtccctg gacgaactgc accgtaaata
     5641 cggcaccgac ctgaaccgtg gactgacctc tgctcgtgct gctgagatct tggctcgtga
     5701 cggtcccaac gctctgaccc ctcctcctac tactcccgaa tggatcaagt tctgccgtca
     5761 gctgttcggt ggtttctccc tgctgctgtg gatcggtgct atcctgtgct tcctggctta
     5821 cgctatcgag actctggtca acggcgaggg caacaacgac aacctgtacc tgggtatcgt
     5881 gctggctgct gtcgtgatcg tgaccggttg cttctcttac taccaagagg ctaagtcctc
     5941 caagatcatg gaatccttca agaacctggt gcctcagcag gctctggtcg tgcgtaacgg
     6001 cgagaagatg tccatcaacg ctgagggtgt cgtcgtgggc gacctggttg aagtgaaagg
     6061 tggcgaccgc atccccgctg acctgcgtat catttccgct cacggttgca aggtggacaa
     6121 ctcctctctg accggcgaat ccgagcctca gactcgttct cctgacttct cccacgagaa
     6181 ccctctggaa acccgtaaca ttgctttctt ctccaccaac tgcgtcgagg gtactgctca
     6241 cggtatcgtg atctccaccg gcgaccgtac tgtgatgggt cgtatcgctt ccctggcttc
     6301 cggactggaa ggtggcaaga cccctatcgc tatggaaatc gagcacttca tccacatcat
     6361 taccggtgtc gctgtgttcc tgggcgtgtc cttcttcatc ctgtctctga tcctcgagta
     6421 cacctggctc gaggctgtga tcttcctgat cggtatcatc gtggctaacg tgcccgaggg
     6481 actgctggct actgtgactg tgtgcctgac tctgaccgct aagcgtatgg ctcgcaagaa
     6541 ctgcctggtc aagaacttgg aggctgtgga aaccctgggt tccacctcca ccatctgctc
     6601 cgacaagacc ggtactctga cccagaaccg tatgaccgtg gctcacatgt ggttcgacaa
     6661 ccagatccac gaggctgaca ccaccgagaa ccagtccggt gcttccttcg acaagtcctc
     6721 cgctacctgg accgctctgt cacgtatcgc tggactgtgc aacagggctg ttttccaggc
     6781 tggccaagag aacgtgccca tcctgaaaag agctgtggct ggcgacgctt ccgagtccgc
     6841 tttgctgaag tgcatcgagc tgtgctgcgg ttccgtgaag cagatgcgtg aacgttaccc
     6901 caaggtggtc gagatcccct tcaactctac caacaagtac cagctgtcca tccacaagaa
     6961 cgctaacccc tccgagtctc gttacctgct ggtcatgaag ggtgctcccg agcgtatcct
     7021 ggaccgttgc tctaccatcc tgatccacgg caaagagcag cctctggacg aggaaatgaa
     7081 ggacgctttc cagaacgctt acctggaact cggtggcctg ggagaacgtg tgctgggatt
     7141 ctgctacctg gctctgcccg acgatcagtt ccctgaggga ttccagttcg acaccgacga
     7201 cctcaacttc cccgtggaca agctgtgctt cgtgggcctg atgtccatga tcgaccctcc
     7261 aagagctgct gtgccagacg ctgttggaaa gtgccgttcc gctggtatca aagtgatcat
     7321 ggtcaccggc gatcacccca tcaccgctaa ggctatcgct aaaggcgtgg gtatcatctc
     7381 cgagggaaac gagactgtcg aggatatcgc tgctcgtctg aacatccccg tgtctcaagt
     7441 gaaccctcgt gacgctaagg cctgcgtggt gcacggttct gacctgaagg acatgacctc
     7501 cgagcagctg gacgacatcc tgctgcacca caccgagatc gtgttcgctc gtacatcccc
     7561 tcagcaaaag ctgatcatcg tcgagggctg ccagcgtcag ggtgctatcg ttgctgttac
     7621 tggcgacggt gtcaacgact cccctgctct gaaaaaggct gacatcggag tggctatggg
     7681 aatcgctggt tccgacgtgt ccaagcaggc tgctgacatg atcctgctcg acgacaactt
     7741 cgcttccatc gtgactggtg tcgaggaagg tcgtctgatc ttcgacaact tgaagaagtc
     7801 gatcgcttac accctgacca gcaacatccc tgagatcacc cctttcttga tcttcatcat
     7861 tgcgaacatc cctctgcctc tgggcaccgt gaccatcctg tgcatcgacc tgggtactga
     7921 catggtgccc gctatctccc tcgcttacga gcaggctgag tccgacatca tgaagcgcca
     7981 gcctcgtaac cccaagaccg acaagctggt taacgagagg ctgatctcca tggcttacgg
     8041 ccagatcggc atgatccagg ctctcggtgg cttcttcacc tacttcgtca tcatggctga
     8101 gaacggtttc tggccctctg gcctgttggg actgagagtg caatgggacg accgttggat
     8161 caacgacgtc gaggactcct acggacagca gtggacttac gaacagcgca agatcgtcga
     8221 gttcacttgc cacaccgctt tcttcgtgtc catcgtggtg gtgcagtggg ctgacctgat
     8281 catctgcaag acccgtcgta actccgtgtt ccagcagggc atgaagaaca agatcctcat
     8341 cttcggcctg ttcgaggaaa ccgctctggc tgctttcctg tcttactgcc ccggcatgga
     8401 tgtggctctg cgtatgtacc ctctgaagct gacctggtgg ttctgcgctt tcccctactc
     8461 tctgttgatc ttcgtgtacg atgaagtccg caagctcatc atccgtcgca accctggtgg
     8521 ctgggtcgag aaagagactt actactaaaa gcttgtcgag aagtactaga ggatcataat
     8581 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     8641 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     8701 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8761 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact
     8821 gcttgagcct aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat
     8881 ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt
     8941 ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg
     9001 cccacagcgg ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat
     9061 gtgacaaacc gtcatcttcg gctacttt
//
