LOCUS       sequence-393162-        3077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with
            a CmrR cargo, as well as a primer binding site for deep sequencing 
            purposes at pTarget. Total transposon size = 1,087 bp..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Lampe GD, King RT, Halpin-Healy TS, Klompe SE, Hogan MI, Vo PLH, 
            Tang S, Chavez A, Sternberg SH
  TITLE     Targeted DNA integration in human cells without double-strand breaks
            using CRISPR-associated transposases.
  JOURNAL   Nat Biotechnol. 2023 Mar 29. doi: 10.1038/s41587-023-01748-1.
  PUBMED    36991112
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Mar 29. doi: 10.1038/s41587-023-01748-1."
COMMENT     Sequence Label: pSL3927 (pDonor(plasmid)_Pse)
FEATURES             Location/Qualifiers
     source          1..3077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      440..1028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     929..948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1182..1199
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             1467..2126
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1533..1552)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        2381..2485
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2486..2554,2555..3077,1..269)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2704..2723)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
       61 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      121 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      181 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      241 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
      301 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
      361 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
      421 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      481 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      541 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      601 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      661 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      721 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      781 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      841 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      901 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      961 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1021 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1081 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1141 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1201 aagcggaaga gcgtgttgat cggctttaag gatctagtgt cgctgcaacc atagtttgac
     1261 ataatgcaac cataccttga cataattaaa gtcatactct gacataaagc cccctggaga
     1321 attcgccacc atgatcagca tgctcgagat cggcacgtaa gaggttccaa ctttcaccat
     1381 aatgcaaggg cgaggaggat aaaataagat cactaccggg cgtatttttt gagttatcga
     1441 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     1501 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     1561 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     1621 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     1681 agttccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     1741 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     1801 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     1861 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     1921 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     1981 ctatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     2041 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     2101 gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttctagag
     2161 tatcctcctc gcccttgtcg gctggttttt ttgtttttag cgttataact tttatttcac
     2221 tatgtcactg tatggttgca ttttatgtca gtgaatggtt gcaggttttt tgtaagttat
     2281 tgatatgcct atccagtgta tgtcacgctt tgcttgcagc gacatctagg atcttagcgc
     2341 gcttttatgg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2401 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2461 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2521 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2581 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2641 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2701 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2761 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2821 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2881 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2941 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3001 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3061 aactggcgaa ctactta
//
