LOCUS       sequence-393220-        1287 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses split-intein fused MSP1E3D1 for circularization during 
            expression (external His-Tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Daniilidis M, Brandl MJ, Hagn F
  TITLE     The Advanced Properties of Circularized MSP Nanodiscs Facilitate 
            High-resolution NMR Studies of Membrane Proteins.
  JOURNAL   J Mol Biol. 2022 Dec 30;434(24):167861. doi: 
            10.1016/j.jmb.2022.167861. Epub 2022 Oct 20.
  PUBMED    36273602
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2022-12-30"; volume: "434(24):167861. doi"; pages: " 
            10.1016/j.jmb.2022.167861. Epub 2022 Oct 20"
COMMENT     Sequence Label: pET28a-cMSP1E3D1
FEATURES             Location/Qualifiers
     source          1..1287
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..51
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1267..1284
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcat gaaaacctgt attttcaggg cattaaaatc
       61 gcgacccgta aatatctggg caaacagaat gtctacgata ttggtgtcga acgtgaccac
      121 aactttgccc tgaaaaatgg ctttatcgca tccaactgct ttaacggttc taccttcagt
      181 aaacttcgcg aacaactggg ccccgtgacg caggaattct gggacaacct ggaaaaagaa
      241 accgagggac tgcgtcagga aatgtccaaa gatttagaag aggtgaaggc caaggttcag
      301 ccatatctcg atgactttca gaaaaaatgg caggaagaga tggaattata tcgtcaaaag
      361 gtggaaccgc tgcgtgcgga actgcaagag ggggcacgcc aaaaactcca tgagctccaa
      421 gagaagctca gcccattagg cgaagaaatg cgcgatcgcg cccgtgcaca tgttgatgca
      481 ctccggactc atttggcgcc atatctcgat gactttcaga aaaaatggca ggaagagatg
      541 gaattatatc gtcaaaaggt ggaaccgctg cgtgcggaac tgcaagaggg ggcacgccaa
      601 aaactccatg agctccaaga gaagctcagc ccattaggcg aagaaatgcg cgatcgcgcc
      661 cgtgcacatg ttgatgcact ccggactcat ttggcgccgt attcggatga acttcgccag
      721 cgtttggccg cacgtctcga ggcgctgaaa gaaaacgggg gtgcccgctt ggctgagtac
      781 cacgcgaaag cgacagaaca cctgagcacc ttgagcgaaa aagcgaaacc ggcgctggaa
      841 gatctacgcc agggcttatt gcctgttctt gagagcttta aagtcagttt tctgtcagct
      901 ctggaagaat atactaaaaa gctgaatacc cagggttgtc tgtcctatga aacggaaatc
      961 ctgacggtgg aatacggtct gctgccgatt ggtaaaattg tggaaaaacg cattgaatgc
     1021 accgtgtata gcgttgacaa caatggcaac atttacacgc agccggtcgc gcaatggcat
     1081 gaccgtggcg aacaggaagt gtttgaatat tgtctggaag atggttctct gatccgcgcc
     1141 accaaagatc acaaatttat gacggtggac ggtcaaatgc tgccgattga tgaaatcttc
     1201 gaacgtgaac tggatctgat gcgcgttgac aacctgccga atgacaagct tgcggccgca
     1261 ctcgagcacc accaccacca ccactga
//
