LOCUS       sequence-393240-        5156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Galectin1 , Excitation: 557, Emission: 585.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5156)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mDsRed-Galectin1-9
FEATURES             Location/Qualifiers
     source          1..5156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     522..541
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(840..859)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    884..931
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1260..1848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1749..1768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2023..2326
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2327..2530
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2481..2501
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2569..2578
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2575..3249
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     2973..2992
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3906..4027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3943..3962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3997..4016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4034..4489)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4171..4192)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4383..4402
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4516..4620
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4622..4979
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4622..4813
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4645..4665)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4830..4965
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5014..5156,1..652)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(5014..5156,1..652)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5068..5087)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
       61 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      121 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      181 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      241 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      301 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      361 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
      421 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
      481 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
      541 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
      601 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
      661 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
      721 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
      781 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
      841 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
      901 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
      961 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     1021 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     1081 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     1141 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1201 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1261 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1321 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1381 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1441 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1501 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1561 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1621 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1681 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1741 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1801 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1861 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1921 ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc
     1981 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     2041 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     2101 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     2161 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2221 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2281 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2341 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2401 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2461 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2521 aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggtcgc caccatggac
     2581 aacaccgagg acgtcatcaa ggagttcatg cagttcaagg tgcgcatgga gggctccgtg
     2641 aacggccact acttcgagat cgagggcgag ggcgagggca agccctacga gggcacccag
     2701 accgccaagc tgcaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     2761 ccccagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     2821 atgaagctgt ccttccccga gggcttcacc tgggagcgct ccatgaactt cgaggacggc
     2881 ggcgtggtgg aggtgcagca ggactcctcc ctgcaggacg gcaccttcat ctacaaggtg
     2941 aagttcaagg gcgtgaactt ccccgccgac ggccccgtaa tgcagaagaa gactgccggc
     3001 tgggagccct ccaccgagaa gctgtacccc caggacggcg tgctgaaggg cgagatctcc
     3061 cacgccctga agctgaagga cggcggccac tacacctgcg acttcaagac cgtgtacaag
     3121 gccaagaagc ccgtgcagct gcccggcaac cactacgtgg actccaagct ggacatcacc
     3181 aaccacaacg aggactacac cgtggtggag cagtacgagc acgccgaggc ccgccactcc
     3241 ggctcccagt ccgggaacat cctcctggac tcaatcatgg cttgtggtct ggtcgccagc
     3301 aacctgaatc tcaaacctgg agagtgcctt cgagtgcgag gcgaggtggc tcctgacgct
     3361 aagagcttcg tgctgaacct gggcaaagac agcaacaacc tgtgcctgca cttcaaccct
     3421 cgcttcaacg cccacggcga cgccaacacc atcgtgtgca acagcaagga cggcggggcc
     3481 tgggggaccg agcagcggga ggctgtcttt cccttccagc ctggaagtgt tgcagaggtg
     3541 tgcatcacct tcgaccaggc caacctgacc gtcaagctgc cagatggata cgaattcaag
     3601 ttccccaacc gcctcaacct ggaggccatc aactacatgg cagctgacgg tgacttcaag
     3661 atcaaatgtg tggcctttga ctgaaatcag ccagcccatg gcccccaata aaggcagctg
     3721 cctctgctcc ctctgctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
     3781 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
     3841 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     3901 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3961 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4021 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     4081 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     4141 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     4201 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     4261 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     4321 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     4381 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     4441 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     4501 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4561 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4621 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4681 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4741 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4801 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4861 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4921 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     4981 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5041 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5101 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttt
//
