LOCUS       sequence-393325-        4741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 507 / 572, Emission: 516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4741)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..4741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             18..812
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             18..812
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(72..91)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     682..701
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1000..1019)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1044..1091
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1420..2008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1909..1928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2183..2486
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2487..2690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2641..2661
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2735..3412
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    3651..3772
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3688..3707)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3742..3761
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3779..4234)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3916..3937)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4128..4147
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4261..4365
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4367..4724
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4367..4558
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4390..4410)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4575..4710
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
       61 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
      121 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
      181 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
      241 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
      301 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
      361 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
      421 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
      481 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
      541 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
      601 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
      661 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
      721 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
      781 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
      841 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
      901 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
      961 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     1021 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     1081 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     1141 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     1201 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     1261 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1321 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1381 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1441 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1501 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1561 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1621 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1681 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1741 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1801 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1861 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1921 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1981 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2041 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2101 taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc
     2161 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2221 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2281 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2341 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2401 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2461 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2521 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2581 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2641 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
     2701 accgtcagat ccgctagcgc taccggtcgc caccatgagt gcgattaagc cagacatgaa
     2761 gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg
     2821 tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag agggcggacc
     2881 tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca gggtattcgc
     2941 caaatatcca gacaacatac aagactattt taagcagtcg tttcctaagg ggtattcgtg
     3001 ggaacgaagc ttgactttcg aagacggggg catttgcaac gccagaaacg acataacaat
     3061 ggaaggggac actttctata ataaagttcg attttatggt accaactttc ccgccaatgg
     3121 tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg
     3181 tgatggagtg ctgacgggtg atattgagat ggctttgttg cttgaaggaa atgcccatta
     3241 ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt taccaggcgc
     3301 ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca acaaggttaa
     3361 gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac gatccggact
     3421 cagatctaag ctgaaccctc ctgatgagag tggccccggc tgcatgagct gcaagtgtgt
     3481 gctctcctga gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat
     3541 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     3601 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     3661 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3721 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
     3781 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     3841 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     3901 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     3961 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     4021 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     4081 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     4141 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     4201 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     4261 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4321 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
     4381 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     4441 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4501 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4561 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4621 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     4681 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
     4741 g
//
