LOCUS       sequence-393408-       10373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock eGFP into human VE-cad locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10373)
  AUTHORS   Bao X, Bhute VJ, Han T, Qian T, Lian X, Palecek SP
  TITLE     Human pluripotent stem cell-derived epicardial progenitors can 
            differentiate to endocardial-like endothelial cells.
  JOURNAL   Bioeng Transl Med. 2017 Jun;2(2):191-201. doi: 10.1002/btm2.10062. 
            Epub 2017 May 22.
  PUBMED    29170757
REFERENCE   2  (bases 1 to 10373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/btm2.10062"; journalName: "Bioeng Transl Med"; date: 
            "2017-06"; volume: "2"; issue: "2"; pages: "191-201"
COMMENT     Sequence Label: VE-cad-eGFP Donor plasmid
FEATURES             Location/Qualifiers
     source          1..10373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(1841..1852)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2093..2149
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(2150..2152,2153..2155,2156..2869)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2150..2152,2153..2155,2156..2866)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2195..2216)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2456..2475)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2803..2824
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    2900..2933
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2994..3493
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3029..3052)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3426..3445
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3533..4132
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3533..3552)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    4689..4722
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6631..6648
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6806..6825)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6807..6825)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6832..6849)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6832..6848)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6841..6863)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6989..7417
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7076..7095)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7286..7307
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             7761..8555
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7815..7834)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8425..8444
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(8573..8641,8642..9433)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8791..8810)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9604..10192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10093..10112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10346..10363
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttttcctg
      301 caggctgcct ccaatctgca atgcagccct tagccagttc atgtgcagga tgcaggatgt
      361 gagctccaac cacctgggtg ctcacggtgg gggatgagcc agggaggagg agggggtggg
      421 actcaggact cctcccccac gcatcaggcc agtatcactt taaaataact tatacataac
      481 agtcactcct tccacttaga tattttcagc atttactgag tgccaggcat tgttctgggc
      541 atgtatttac ataaaaccta tttttgaaaa aggaaaaagg atccagttct aaaatggaaa
      601 gtttaaaaac ttctagttta gattgtctcc acgtgctttc tttcttttta tagaattcct
      661 ccggccttga tacatatttt ttttcttgtt tgggcctcag aaattttagg agcattgtag
      721 gccttagttt ggatttccca gggagcagcc ctgggatgag gattggggtg gaagtggttt
      781 atttgggagg tggtttattt ccctggatac ctcctaaggc agagggggag tgaagctggg
      841 aagggaggag gccagcgaag gtggaatgtt cccatcgtga ggaactggag cctcctccca
      901 tagggttgcc gggaggcagc gtggggccac cccccagcat catcccaccg ggggagcaca
      961 gtgatgtact gtatcctgca tttcactcgc catgagacgc agcaggggct gatcctccag
     1021 aggaagccct caggtgatta gagatcaccc tggcactcac ggcaaggcct ggatacagcc
     1081 acggggcacc tgcaatacag attccaggcc tctcctgcct gtagccctcc tgagagtggc
     1141 ctcggtatgc cccacttctg ggacttctcc agcagagatt tctcctttat taataattaa
     1201 tgatgatgat ggcatcccca ccacaaatag aattgcattt gacctcacaa ccatcctccc
     1261 aaatataaac agagcaggga ggtcatcatt tccattttac agacaaggaa actgaggctg
     1321 gggggcaggg ctgccttcca gggcatccca gctttactta tgtatatagt agtttgttca
     1381 gtaactcaaa gctagaaggc ctgagcctaa ttttagaagc tctctgcact tcaaggtcta
     1441 cccctcctta gggatccaag accctcacat agccacaccc ctcagagatt cccacaggct
     1501 gagcatccgc acctgcccct gggtgtgggg gaacggcagc ggtgggagtg tcgggcgagg
     1561 tggggatggg ggcatggggg gccgcccagg cccccagcct tgtctgactc tgctgctcgg
     1621 ctccctggct gcagtgatca ccctgctcat cttcctgcgg cggcggctcc ggaagcaggc
     1681 ccgcgcgcac ggcaagagcg tgccggagat ccacgagcag ctggtcacct acgacgagga
     1741 gggcggcggc gagatggaca ccaccagcta cgatgtgtcg gtgctcaact cggtgcgccg
     1801 cggcggggcc aagcccccgc ggcccgcgct ggacgcccgg ccttccctct atgcgcaggt
     1861 gcagaagcca ccgaggcacg cgcctggggc acacggaggg cccggggaga tggcagccat
     1921 gatcgaggtg aagaaggacg aggcggacca cgacggcgac ggccccccct acgacacgct
     1981 gcacatctac ggctacgagg gctccgagtc catagccgag tccctcagct ccctgggcac
     2041 cgactcatcc gactctgacg tggattacga cttccttaac gactgggcta gcgccactaa
     2101 cttctccctg ttgaaacaag caggggatgt cgaagagaat cccgggccaa tggtgagcaa
     2161 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     2221 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     2281 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     2341 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     2401 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     2461 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     2521 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     2581 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     2641 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     2701 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     2761 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     2821 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag aattccgatc
     2881 atattcaata acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtct
     2941 gaagaggagt ttacgtccag ccaagcttag gatctcgacc tcgaaattct accgggtagg
     3001 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     3061 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     3121 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     3181 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     3241 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     3301 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     3361 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     3421 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     3481 tccgggcctt tcgacctgca tccatctaga tctcgagcag ctgaagctta ccatgaccga
     3541 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     3601 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     3661 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     3721 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     3781 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     3841 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     3901 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     3961 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     4021 cgcgccccac aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     4081 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     4141 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga tgatatcaga
     4201 tccccgggat gcagaaattg atgatctatt aaacaataaa gatgtccact aaaatggaag
     4261 tttttcctgt catactttgt taagaagggt gagaacagag tacctacatt ttgaatggaa
     4321 ggattggagc tacgggggtg ggggtggggt gggattagat aaatgcctgc tctttactga
     4381 aggctcttta ctattgcttt atgataatgt ttcatagttg gatatcataa tttaaacaag
     4441 caaaaccaaa ttaagggcca gctcattcct cccactcatg atctatagat ctatagatct
     4501 ctcgtgggat cattgttttt ctcttgattc ccactttgtg gttctaagta ctgtggtttc
     4561 caaatgtgtc agtttcatag cctgaagaac gagatcagca gcctctgttc cacatacact
     4621 tcattctcag tattgttttg ccaagttcta attccatcag aagctggtcg agatccggaa
     4681 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgaagaggt
     4741 tcactaggcg cgcccctcca aaaatggcag tgactcccca gcccagcacc ccttcctcgt
     4801 gggtcccaga gacctcatca gccttgggat agcaaactcc aggttcctga aatatccagg
     4861 aatatatgtc agtgatgact attctcaaat gctggcaaat ccaggctggt gttctgtctg
     4921 ggctcagaca tccacataac cctgtcaccc acagaccgcc gtctaactca aagacttcct
     4981 ctggctcccc aaggctgcaa agcaaaacag actgtgttta actgctgcag ggtctttttc
     5041 tagggtccct gaacgccctg gtaaggctgg tgaggtcctg gtgcctatct gcctggaggc
     5101 aaaggcctgg acagcttgac ttgtggggca ggattctctg cagcccattc ccaagggaga
     5161 ctgaccatca tgccctctct cgggagccct agccctgctc caactccata ctccactcca
     5221 agtgccccac cactccccaa cccctctcca ggcctgtcaa gagggaggaa ggggccccat
     5281 ggcagctcct gaccttgggt cctgaagtga cctcactggc ctgccatgcc agtaactgtg
     5341 ctgtactgag cactgaacca cattcaggga aatggcttat taaactttga agcaactgtg
     5401 aattcattct ggaggggcag tggagatcag gagtgacaga tcacagggtg agggccacct
     5461 ccacacccac cccctctgga gaaggcctgg aagagctgag accttgcttt gagactcctc
     5521 agcacccctc cagttttgcc tgagaagggg cagatgttcc cggagcagaa gacgtctccc
     5581 cttctctgcc tcacctggtc gccaatccat gctctctttc ttttctctgt ctactcctta
     5641 tcccttggtt tagaggaacc caagatgtgg cctttagcaa aactggacaa tgtccaaacc
     5701 cactcatgac tgcatgacgg agccgagcca tgtgtcttta cacctcgctg ttgtcacatc
     5761 tcagggaact gaccctcagg cacaccttgc agaaggcaag gccctgccct gcccaacctc
     5821 tgtggtcacc catgcatctt ccactggaac gtttcactgc aaacacacct tggagaagtg
     5881 gcatcagtca acagagaggg gcagggaagg agacaccaag ctcacccttc gtcatggacc
     5941 gaggttccca ctctgggcaa agcacctcac actgcaaggg attgtagata acactgactt
     6001 gtttgtttta accaataact agcttcttat aatgattttt ttactaatga tacttacaag
     6061 tttctagctc tcacagacat atagaataag ggtttttgca taataagcag gttgttattt
     6121 aggttaacaa tattaattca ggttttttag ttggaaaaac aattcctgta accttctatt
     6181 ttctataatt gtagtaattg ctctacagat aatgtctata tattggccaa actggtgcat
     6241 gacaagtact gtattttttt atacctaaat aaagaaaaat ctttagcctg ggcaacatag
     6301 ggagacccta tctctaccaa aaaaaaatta attagccagg catggtggca ggcacctgtg
     6361 gtcccagcta cttgggaggc tgagatagga gaatcacttc agcctgggag atcaaggctg
     6421 cagtgagctc tgaacgcacc actacactcc agcctgggtg acagagtgag atcctgtctc
     6481 aaaaaaagag aaagaaaacc tttgagattc ttccattttt agagctgaga gagcacttgt
     6541 gaaacacaca cacatgcaca aacatataaa catgcataca ggcatgcaca tgcacacaca
     6601 aatacacata cacacacaca agcacacaca caccaccacc accatcatca gaggaactta
     6661 cagaaaaggg gacatttata gattcctagg aatatgccaa agcttttcaa agcctctatg
     6721 gacagcggcc ggccaaaagg gcgaattctg cagatatcca tcacactggc ggccgctcga
     6781 gcatgcatct agagggccca attcgcccta tagtgagtcg tattacaatt cactggccgt
     6841 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6901 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6961 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     7021 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7081 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7141 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7201 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7261 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7321 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7381 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaattca gggcgcaagg
     7441 gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
     7501 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
     7561 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     7621 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
     7681 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga
     7741 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     7801 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     7861 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     7921 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     7981 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     8041 tgggcgaagt gccggggcag gatctcctgt catcccacct tgctcctgcc gagaaagtat
     8101 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     8161 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     8221 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     8281 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     8341 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     8401 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     8461 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     8521 tcgccttcta tcgccttctt gacgagttct tctgaattga aaaaggaaga gtatgagtat
     8581 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8641 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8701 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8761 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8821 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8881 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8941 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9001 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9061 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9121 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9181 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9241 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9301 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9361 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9421 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9481 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9541 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9601 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9661 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9721 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9781 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9841 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9901 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9961 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10021 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    10081 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    10141 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    10201 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    10261 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    10321 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag
//
