LOCUS       sequence-393577-       17683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x 
            Csy4-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd 
            and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17683)
  AUTHORS   Miller SS, Dornbusch MR, Farmer AD, Huertas R, Gutierrez-Gonzalez 
            JJ, Young ND, Samac DA, Curtin SJ
  TITLE     Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate 
            phosphate.
  JOURNAL   G3 (Bethesda). 2022 May 30;12(6). pii: 6574359. doi: 
            10.1093/g3journal/jkac096.
  PUBMED    35471600
REFERENCE   2  (bases 1 to 17683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2022-05-30"; volume: "12(6). pii: 6574359. 
            doi"; pages: " 10.1093/g3journal/jkac096"
COMMENT     Sequence Label: pTRANS-Pho2-1/2-2-Csy4
FEATURES             Location/Qualifiers
     source          1..17683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1843..2640)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1954..1973)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2564..2583
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2703..3379)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    3570..3591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3606..3611,3612..3629,3630..3636)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3644..3660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3649..3671
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3668..3684
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3668..3684
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4214..4559
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5151..5207
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5208..9311
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5208..9311
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9324..9344
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      9357..9605
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     protein_bind    10105..10124
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        10145..10220
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    10221..10240
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        10261..10336
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    10337..10356
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        10377..10452
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    10453..10472
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        10493..10568
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    10569..10588
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        10609..10684
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    10685..10704
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        10725..10800
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    10801..10820
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     polyA_signal    10840..11014
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     11061..11077
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12694..12711)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12694..12710)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12703..12725)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    12913..12937
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14238..14867
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             15296..16369
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      16435..16629
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(16865..16887)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    16973..17113
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     17028..17047
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(17128..17145)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(17299..17683,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17379..17398)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
     1861 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     1921 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     1981 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     2041 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
     2101 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     2161 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     2221 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     2281 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     2341 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     2401 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
     2461 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     2521 cagccgaaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     2581 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
     2641 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     2701 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     2761 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     2821 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     2881 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
     2941 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
     3001 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
     3061 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
     3121 accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     3181 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     3241 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     3301 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     3361 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     3421 ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa
     3481 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3541 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3601 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3661 tttcacacag gaaacagcta tgacatgatt acgaattcga gctcggtacc cggggatcgg
     3721 cgcgccagat ttgccttttc aatttcagaa agaatgctaa cccacagatg gttagagagg
     3781 cttacgcagc aggtatcatc aagacgatct acccgagcaa taatctccag gaaatcaaat
     3841 accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca
     3901 cagagaaaga tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct
     3961 tgcttcacaa accaaggcaa gtaatagaga ttggagtctc taaaaaggta gttcccactg
     4021 aatcaaaggc catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga
     4081 ctggcgaaca gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca
     4141 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
     4201 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
     4261 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
     4321 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc
     4381 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     4441 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     4501 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac
     4561 gggggactcc tgcaggaaaa atggatcatt atcttgatat tagacttaga cctgatccag
     4621 aatttccacc agctcaactt atgtctgttc tttttggaaa acttcatcaa gctcttgttg
     4681 ctcaaggagg agatagaatt ggagtttctt ttcctgatct tgatgaatca agatcaagac
     4741 ttggagaaag acttagaatt catgcttctg ctgatgatct tagagctttg cttgctagac
     4801 cttggcttga aggacttaga gatcatcttc aatttggaga accagctgtt gttccacatc
     4861 caactcctta tagacaagtt tcaagagttc aagctaaatc taatccagaa agacttagaa
     4921 gaagacttat gagaagacat gatctttctg aagaagaagc tagaaaaaga attcctgata
     4981 ctgttgctag agctttggat ttgccttttg ttacacttag atcacaatct actggacaac
     5041 attttagact ttttattaga catggaccac ttcaagttac tgctgaagaa ggaggattta
     5101 cttgttatgg actttctaag ggaggttttg ttccttggtt tggatctgga gctactaatt
     5161 tttctcttct taagcaagct ggagatgttg aagaaaatcc tggacccatg gataagaagt
     5221 actctatcgg actcgatatc ggaactaact ctgtgggatg ggctgtgatc accgatgagt
     5281 acaaggtgcc atctaagaag ttcaaggttc tcggaaacac cgataggcac tctatcaaga
     5341 aaaaccttat cggtgctctc ctcttcgatt ctggtgaaac tgctgaggct accagactca
     5401 agagaaccgc tagaagaagg tacaccagaa gaaagaacag gatctgctac ctccaagaga
     5461 tcttctctaa cgagatggct aaagtggatg attcattctt ccacaggctc gaagagtcat
     5521 tcctcgtgga agaagataag aagcacgaga ggcaccctat cttcggaaac atcgttgatg
     5581 aggtggcata ccacgagaag taccctacta tctaccacct cagaaagaag ctcgttgatt
     5641 ctactgataa ggctgatctc aggctcatct acctcgctct cgctcacatg atcaagttca
     5701 gaggacactt cctcatcgag ggtgatctca accctgataa ctctgatgtg gataagttgt
     5761 tcatccagct cgtgcagacc tacaaccagc ttttcgaaga gaaccctatc aacgcttcag
     5821 gtgtggatgc taaggctatc ctctctgcta ggctctctaa gtcaagaagg cttgagaacc
     5881 tcattgctca gctccctggt gagaagaaga acggactttt cggaaacttg atcgctctct
     5941 ctctcggact cacccctaac ttcaagtcta acttcgatct cgctgaggat gcaaagctcc
     6001 agctctcaaa ggatacctac gatgatgatc tcgataacct cctcgctcag atcggagatc
     6061 agtacgctga tttgttcctc gctgctaaga acctctctga tgctatcctc ctcagtgata
     6121 tcctcagagt gaacaccgag atcaccaagg ctccactctc agcttctatg atcaagagat
     6181 acgatgagca ccaccaggat ctcacacttc tcaaggctct tgttagacag cagctcccag
     6241 agaagtacaa agagattttc ttcgatcagt ctaagaacgg atacgctggt tacatcgatg
     6301 gtggtgcatc tcaagaagag ttctacaagt tcatcaagcc tatcctcgag aagatggatg
     6361 gaaccgagga actcctcgtg aagctcaata gagaggatct tctcagaaag cagaggacct
     6421 tcgataacgg atctatccct catcagatcc acctcggaga gttgcacgct atccttagaa
     6481 ggcaagagga tttctaccca ttcctcaagg ataacaggga aaagattgag aagattctca
     6541 ccttcagaat cccttactac gtgggacctc tcgctagagg aaactcaaga ttcgcttgga
     6601 tgaccagaaa gtctgaggaa accatcaccc cttggaactt cgaagaggtg gtggataagg
     6661 gtgctagtgc tcagtctttc atcgagagga tgaccaactt cgataagaac cttccaaacg
     6721 agaaggtgct ccctaagcac tctttgctct acgagtactt caccgtgtac aacgagttga
     6781 ccaaggttaa gtacgtgacc gagggaatga ggaagcctgc ttttttgtca ggtgagcaaa
     6841 agaaggctat cgttgatctc ttgttcaaga ccaacagaaa ggtgaccgtg aagcagctca
     6901 aagaggatta cttcaagaaa atcgagtgct tcgattcagt tgagatttct ggtgttgagg
     6961 ataggttcaa cgcatctctc ggaacctacc acgatctcct caagatcatt aaggataagg
     7021 atttcttgga taacgaggaa aacgaggata tcttggagga tatcgttctt accctcaccc
     7081 tctttgaaga tagagagatg attgaagaaa ggctcaagac ctacgctcat ctcttcgatg
     7141 ataaggtgat gaagcagttg aagagaagaa gatacactgg ttggggaagg ctctcaagaa
     7201 agctcattaa cggaatcagg gataagcagt ctggaaagac aatccttgat ttcctcaagt
     7261 ctgatggatt cgctaacaga aacttcatgc agctcatcca cgatgattct ctcaccttta
     7321 aagaggatat ccagaaggct caggtttcag gacagggtga tagtctccat gagcatatcg
     7381 ctaacctcgc tggatctcct gcaatcaaga agggaatcct ccagactgtg aaggttgtgg
     7441 atgagttggt gaaggtgatg ggaaggcata agcctgagaa catcgtgatc gaaatggcta
     7501 gagagaacca gaccactcag aagggacaga agaactctag ggaaaggatg aagaggatcg
     7561 aggaaggtat caaagagctt ggatctcaga tcctcaaaga gcaccctgtt gagaacactc
     7621 agctccagaa tgagaagctc tacctctact acctccagaa cggaagggat atgtatgtgg
     7681 atcaagagtt ggatatcaac aggctctctg attacgatgt tgatcatatc gtgccacagt
     7741 cattcttgaa ggatgattct atcgataaca aggtgctcac caggtctgat aagaacaggg
     7801 gtaagagtga taacgtgcca agtgaagagg ttgtgaagaa aatgaagaac tattggaggc
     7861 agctcctcaa cgctaagctc atcactcaga gaaagttcga taacttgact aaggctgaga
     7921 ggggaggact ctctgaattg gataaggcag gattcatcaa gaggcagctt gtggaaacca
     7981 ggcagatcac taagcacgtt gcacagatcc tcgattctag gatgaacacc aagtacgatg
     8041 agaacgataa gttgatcagg gaagtgaagg ttatcaccct caagtcaaag ctcgtgtctg
     8101 atttcagaaa ggatttccaa ttctacaagg tgagggaaat caacaactac caccacgctc
     8161 acgatgctta ccttaacgct gttgttggaa ccgctctcat caagaagtat cctaagctcg
     8221 agtcagagtt cgtgtacggt gattacaagg tgtacgatgt gaggaagatg atcgctaagt
     8281 ctgagcaaga gatcggaaag gctaccgcta agtatttctt ctactctaac atcatgaatt
     8341 tcttcaagac cgagattacc ctcgctaacg gtgagatcag aaagaggcca ctcatcgaga
     8401 caaacggtga aacaggtgag atcgtgtggg ataagggaag ggatttcgct accgttagaa
     8461 aggtgctctc tatgccacag gtgaacatcg ttaagaaaac cgaggtgcag accggtggat
     8521 tctctaaaga gtctatcctc cctaagagga actctgataa gctcattgct aggaagaagg
     8581 attgggaccc taagaaatac ggtggtttcg attctcctac cgtggcttac tctgttctcg
     8641 ttgtggctaa ggttgagaag ggaaagagta agaagctcaa gtctgttaag gaacttctcg
     8701 gaatcactat catggaaagg tcatctttcg agaagaaccc aatcgatttc ctcgaggcta
     8761 agggatacaa agaggttaag aaggatctca tcatcaagct cccaaagtac tcactcttcg
     8821 aactcgagaa cggtagaaag aggatgctcg cttctgctgg tgagcttcaa aagggaaacg
     8881 agcttgctct cccatctaag tacgttaact ttctttacct cgcttctcac tacgagaagt
     8941 tgaagggatc tccagaagat aacgagcaga agcaactttt cgttgagcag cacaagcact
     9001 acttggatga gatcatcgag cagatctctg agttctctaa aagggtgatc ctcgctgatg
     9061 caaacctcga taaggtgttg tctgcttaca acaagcacag agataagcct atcagggaac
     9121 aggcagagaa catcatccat ctcttcaccc ttaccaacct cggtgctcct gctgctttca
     9181 agtacttcga tacaaccatc gataggaaga gatacacctc taccaaagaa gtgctcgatg
     9241 ctaccctcat ccatcagtct atcactggac tctacgagac taggatcgat ctctcacagc
     9301 tcggtggtga ttcaagggct gatcctaaga agaagaggaa ggtttgacgt cgacgatatg
     9361 aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt
     9421 tttttcttgg cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag
     9481 atcacattat aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc
     9541 tcttctgcag catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag
     9601 ataagccaga gctctggtga cggacggcgc gctggcagac atactgtccc acaaatgaag
     9661 atggaatctg taaaagaaaa cgcgtgaaat aatgcgtctg acaaaggtta ggtcggctgc
     9721 ctttaatcaa taccaaagtg gtccctacca cgatggaaaa actgtgcagt cggtttggct
     9781 ttttctgacg aacaaataag attcgtggcc gacaggtggg ggtccaccat gtgaaggcat
     9841 cttcagactc caataatgga gcaatgacgt aagggcttac gaaataagta agggtagttt
     9901 gggaaatgtc cactcacccg tcagtctata aatacttagc ccctccctca ttgttaaggg
     9961 agcaaaatct cagagagata gtcctagaga gagaaagaga gcaagtagcc tagaagtagt
    10021 caaggcggcg aagtattcag gcacgtggcc aggaagaaga aaagccaaga cgacgaaaac
    10081 aggtaagagc taagcttcct gcaggttcac tgccgtatag gcagattaga acctaaagga
    10141 accggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
    10201 aagtggcacc gagtcggtgc gttcactgcc gtataggcag aattgggaag ataaagacca
    10261 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
    10321 ggcaccgagt cggtgcgttc actgccgtat aggcaggttt gagtctccta aatacggttt
    10381 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
    10441 ccgagtcggt gcgttcactg ccgtataggc agattatgta actaaggggc cagttttaga
    10501 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
    10561 gtcggtgcgt tcactgccgt ataggcagtc acacgcaaac tggcagctgt tttagagcta
    10621 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
    10681 gtgcgttcac tgccgtatag gcaggttcaa aggccgctcg aggagtttta gagctagaaa
    10741 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
    10801 gttcactgcc gtataggcag gtcgatcgac aagctcgagt ttctccataa taatgtgtga
    10861 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
    10921 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
    10981 cctaaaacca aaatccagta ctaaaatcca gatcccccga attagagctc taccggcgta
    11041 atatggcgcg ccgaagagcg cgaggtcgac ggtatcgata agcttgatat cgaattcctg
    11101 cagcccgggg gatccgcccg ctctagactg ccttgtaggt gcgaattttc tctgctcaaa
    11161 ttgttgaggt tagcggattt gtaaacgcgt ttatatgggc tgcttggagg gtacttttgg
    11221 attaattttt ttctgccagc gcattctgac gcggcaccgc tttggaaagt gcgctgtggg
    11281 tccgcgtttt ctacaataat gtgccgatcc ggtcagaaag tatatggatg agttgtgcca
    11341 gcctcaccaa cgtgctgcag gcccatcatg actacttcaa tgttaatggg ggtaatgaat
    11401 aaataggcga aattgggttc acggtgggcc cagggaatat aatattgccg cagaggtagt
    11461 cggatgccaa ggcccgcaac taatagttca cgaacaaatt cattgtagtg ggcggccaac
    11521 tccaaaacca attgccagtt attgtattgc aatacatata tggcttcctc ccttgcctgc
    11581 aggatgtctg agccacctcg ggctgagacc tttgtattcc tggacctaga agccactggg
    11641 ctcccaaaca tggaccctga gattgcagag atatcccttt ttgctgttca ccgctcttcc
    11701 ctggagaacc cagaacggga tgattctggt tccttggtgc tgccccgtgt tctggacaag
    11761 ctcacactgt gcatgtgccc ggagcgcccc tttactgcca aggccagtga gattactggt
    11821 ttgagcagcg aaagcctgat gcactgcggg aaggctggtt tcaatggcgc tgtggtaagg
    11881 acactgcagg gcttcctaag ccgccaggag ggccccatct gccttgtggc ccacaatggc
    11941 ttcgattatg acttcccact gctgtgcacg gagctacaac gtctgggtgc ccatctgccc
    12001 caagacactg tctgcctgga cacactgcct gcattgcggg gcctggaccg tgctcacagc
    12061 cacggcacca gggctcaagg ccgcaaaagc tacagcctgg ccagtctctt ccaccgctac
    12121 ttccaggctg aacccagtgc tgcccattca gcagaaggtg atgtgcacac cctgcttctg
    12181 atcttcctgc atcgtgctcc tgagctgctc gcctgggcag atgagcaggc ccgcagctgg
    12241 gctcatattg agcccatgta cgtgccacct gatggtccaa gcctcgaagc ctgataactc
    12301 gaggttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt
    12361 aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag
    12421 aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt
    12481 ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat tttgttttga
    12541 gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacact
    12601 tgtagttgtg agctcgcgcg tagtcctcgg taatatcgca gaacaaaagt acctgatatc
    12661 gagtgtactt caagtcacac cggcgagtgt ggcactggcc gtcgttttac aacgtcgtga
    12721 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
    12781 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
    12841 tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa
    12901 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
    12961 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
    13021 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
    13081 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
    13141 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
    13201 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
    13261 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
    13321 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
    13381 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
    13441 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
    13501 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
    13561 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
    13621 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
    13681 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
    13741 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
    13801 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
    13861 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
    13921 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
    13981 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
    14041 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
    14101 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
    14161 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
    14221 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
    14281 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
    14341 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
    14401 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
    14461 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
    14521 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
    14581 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
    14641 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
    14701 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
    14761 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
    14821 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
    14881 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
    14941 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
    15001 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
    15061 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
    15121 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
    15181 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
    15241 gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
    15301 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
    15361 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
    15421 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
    15481 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
    15541 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
    15601 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
    15661 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
    15721 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
    15781 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
    15841 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
    15901 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
    15961 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
    16021 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
    16081 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
    16141 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
    16201 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
    16261 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
    16321 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
    16381 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
    16441 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
    16501 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
    16561 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
    16621 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
    16681 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
    16741 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
    16801 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
    16861 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    16921 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
    16981 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
    17041 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    17101 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    17161 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    17221 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    17281 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    17341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    17401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    17461 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    17521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    17581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    17641 actggcagca gccactggta acaggattag cagagcgagg tat
//
