LOCUS       sequence-39390-e         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Weinman EJ, Wang Y, Wang F, Greer C, Steplock D, Shenolikar S
  TITLE     A C-terminal PDZ motif in NHE3 binds NHERF-1 and enhances cAMP 
            inhibition of sodium-hydrogen exchange.
  JOURNAL   Biochemistry. 2003 Nov 4. 42(43):12662-8.
  PUBMED    14580213
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2003 Nov 4. 42(43):12662-8."
COMMENT     Sequence Label: pcDNA-N1-PDZ1-GAGA
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             98..120
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             131..148
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             158..175
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             182..226
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             242..256
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 agcagagctc tctggctact agaagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag aaataatttt gtttaacttt aagaaggaga
      121 tatacatatg caccatcatc atcatcattc ttctggtctg gtgccacgcg gttctggtat
      181 gaaagaaacc gctgctgcta aattcgaacg ccagcacatg gacagcccag atctgggtac
      241 cgacgacgac gacaaggcca tggctgatcg cgccgctgag ccgtcgcggg gcgagatgag
      301 cgcggacgcg gcggccgggg cgcccctgcc ccggctctgc tgcctggaga agggccacaa
      361 cggcgccggc gcccacctgc atggggagaa gggcaaggtg ggccagtaca tccggctggt
      421 ggagcccggc tctccggccg agaaggcggg gctgctggcc ggggaccggc tggtggaggt
      481 gaacggcgag aacgtggaga aggagaccca ccagcaggtg gtgagccgca tccgcgccgc
      541 gctcaacgcc gtgcgcctgc tggtggtcga ccccgacacg gacgagcagt tcaggaagct
      601 cggagtccag atccgggggg agctgctacg cgcccaggcg gggccggagc aggccgggcc
      661 gccggccgcc cccggcgagc agggcccggc cggtgagaac gagccgcgcg aggtcgagaa
      721 gagccacccg gagcggcgc
//
