LOCUS       sequence-394149-        3558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression plasmid for recombinant His-Myc tag mouse PLD4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Gavin AL, Huang D, Blane TR, Thinnes TC, Murakami Y, Fukui R, Miyake
            K, Nemazee D
  TITLE     Cleavage of DNA and RNA by PLD3 and PLD4 limits autoinflammatory 
            triggering by multiple sensors.
  JOURNAL   Nat Commun. 2021 Oct 7;12(1):5874. doi: 10.1038/s41467-021-26150-w.
  PUBMED    34620855
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-10-7"; volume: "12(1):5874. doi"; pages: " 
            10.1038/s41467-021-26150-w"
COMMENT     Sequence Label: SuExp His-Myc-mPLD4
FEATURES             Location/Qualifiers
     source          1..3558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        47..426
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        427..630
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     581..601
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     627..651
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          740..836
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             852..869
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             897..926
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             927..956
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             963..974
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(1902..1913)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(2264..2275)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    2338..2472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2362..2381)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2416..2435
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(2515..3103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2595..2614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3128..3502)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(join(3503..3558,1..8))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatacataac gcgtcgcgag gccatatggg ttaacccatg gccgttgaca ttgattattg
       61 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
      121 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      181 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      241 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      301 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      361 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      421 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      481 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      541 cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt
      601 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
      661 cgtcgagccg agtggttgtg cctccataga agccaccatg acccgcctga ccgtgctggc
      721 cctgctggcc ggcctgctgg taagtttagt ctttttgtct tttatttcag gtcccggatc
      781 cggtggtggt gcaaatcaaa gaactgctcc tcagtggatg ttgcctttac ttctaggcca
      841 gcagccgcgc ccatcatcat catcatcatg gtccgctagt tgacgtcgcc agcaatgaac
      901 aaaaactcat ctcagaagag gatctggcta gtatgactgg tggacagcaa atgggtcggg
      961 atatcgaagg tcgtgggcta gcatggcaag gggccacttc tttcaccagt catcggatgt
     1021 tccctgagga agtgccctcc tggtcctggg agaccctgaa aggagacgct gagcagcaga
     1081 ataactcctg tcagctcatc cttgtggaaa gcatccccga ggacttgcca tttgcagctg
     1141 gcagccccac tgcccagccc ctggcccagg cttggctgca gcttcttgac actgctcggg
     1201 agagcgtcca cattgcctcg tactactggt ccctcactgg actggacatt ggagtcaatg
     1261 actcgtcttc tcggcaggga gaggcccttc tacagaagtt ccaacagctt cttctcagga
     1321 acatctctgt ggtggtggcc acccacagcc caacattggc caagacatcc actgacctcc
     1381 aggtcttggc tgcccatggt gcccagatac gacaagtgcc catgaaacag cttactgggg
     1441 gtgttctaca ctccaaattc tgggttgtgg atgggcgaca cgtctacgtg ggcagcgcca
     1501 acatggactg gcggtccctg actcaggtga aggaacttgg tgcaatcatc tacaactgca
     1561 gcaacctggc tcaagacctt gagaaaacat tccagaccta ctgggtgcta gggactcccc
     1621 aagctgttct ccctaaaacc tggcctcgga acttctcatc ccacatcaac cgcttccatc
     1681 ccttgcgggg tccctttgat ggggttccca ccacggccta tttctcggcc tcccctccct
     1741 ccctctgccc gcatggccgg acccgggatc tggacgcagt gttgggagtg atggagggtg
     1801 ctcgccagtt catctatgtc tcggtgatgg agtatttccc taccacgcgc ttcacccacc
     1861 atgccaggta ctggcccgtg ctggacaatg cgctacgggc agcggccctc aataagggtg
     1921 tgcatgtgcg cttactggtc agctgctggt tcaacacaga ccccaccatg ttcgcttatc
     1981 tgaggtccct gcaggctttc agtaacccct cggctggcat ctcagtggat gtgaaagtct
     2041 tcatcgtgcc tgtgggaaat cattccaaca tcccgttcag ccgcgtgaac cacagcaagt
     2101 tcatggtcac agacaagaca gcctatgtag gcacctctaa ctggtcagaa gactacttca
     2161 gccacaccgc tggtgtgggc ctgattgtca gccagaagac ccccagagcc cagccaggcg
     2221 caaccaccgt gcaggagcag ctgaggcaac tctttgaacg agactggagt tcccactatg
     2281 ctatggacct agacagacaa gtcccgagcc aggactgtgt ctggtaatct agaagctgat
     2341 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     2401 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     2461 aataaacaag ttggatatcc tcgagactag tgggcccgtt taaacacatg tgtttttcca
     2521 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2581 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2641 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2701 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2761 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2821 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2881 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2941 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3001 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3061 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3121 ttctacgtca gtcctgctcc tcggccacga agtgcacgca gttgccggcc gggtcgcgca
     3181 gggcgaactc ccgcccccac ggctgctcgc cgatctcggt catggccggc ccggaggcgt
     3241 cccggaagtt cgtggacacg acctccgacc actcggcgta cagctcgtcc aggccgcgca
     3301 cccacaccca ggccagggtg ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca
     3361 gggtcacgtc gtcccggacc acaccggcga agtcgtcctc cacgaagtcc cgggagaacc
     3421 cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa
     3481 cggcactggt caacttggcc atactcttcc tttttcaata ttattgaagc atttatcagg
     3541 gttattgtct catgagcg
//
