LOCUS       sequence-394444-       11102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual barcoding cassettes for SSN-seq; Barcode Set 7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Lu X, Lofgren SM, Zhao Y, Mazur PK
  TITLE     Multiplexed transcriptomic profiling of the fate of human CAR T 
            cells in vivo via genetic barcoding with shielded small nucleotides.
  JOURNAL   Nat Biomed Eng. 2023 Aug 31. doi: 10.1038/s41551-023-01085-3.
  PUBMED    37652986
REFERENCE   2  (bases 1 to 11102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng. 2023 Aug 31. doi: 10.1038/s41551-023-01085-3."
COMMENT     Sequence Label: pXL_dSSN7_EF1a-tNGFR-2A-Puro
FEATURES             Location/Qualifiers
     source          1..11102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1990..2238
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1990..2010
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2161..2180
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(2622..2655)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2659..2679
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        2891..4053
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3122..4044
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4001..4021
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4064..4073
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4910..4966
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4967..5563
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5460..5480
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5597..6185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5650..5670)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6068..6079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6257..6490
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6516..6533)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6522..6746
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6792..7220
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6879..6898)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7089..7110
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7229..7249)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7394..7528
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7455..7474
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7590..7637
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7656..8030
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8160..8281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8197..8216)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8251..8270
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8330..8346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8330..8346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8343..8365)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8354..8370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8378..8384,8385..8402,8403..8408))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8423..8444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8561..8578)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8732..9320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8812..8831)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9491..10282,10283..10351))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10114..10133
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10352..10456)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10531..10550)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10722..11101
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2041 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
     2101 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     2161 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     2221 tggaaaggac gaaacaccgt gctcgcttcg gcagcacata tactagtcga ccctgaaggg
     2281 tttaagagct aagctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact
     2341 tggccgcttt aaggccggtc ctagcaaggc caagtggcac cgagtcggtg ctctagagcg
     2401 gacttcggtc cgcttttttt ctcgagtgat ccgacgcgcc atctctaggc ccgcgccggc
     2461 cccctcgcac ggacttgtgg gagaagctcg gctactcccc tgccccggtt aatttgcata
     2521 taatatttcc tagtaactat agaggcttaa tgtgcgataa aagacagata atctgttctt
     2581 tttaatacta gctacatttt acatgatagg cttggatttc tataacttcg tatagcatac
     2641 attatacgaa gttataaaca gcacaaaagg aaactcaccc taactgtaaa gtaattgtgt
     2701 gttttgagac tataagtatc ccttggagaa ccaccttgtt ggtgctcgct tcggcagcac
     2761 atatactagt cgaccctgaa ggttgccatg tgtatcggtc cgatactctg atgatgggct
     2821 ttaaggccgg tcctagcaac catcattcat ggcaatctag agcggacttc ggtccgcttt
     2881 ttttgaattc ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     2941 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     3001 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     3061 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     3121 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     3181 gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc tggagccagg
     3241 ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     3301 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     3361 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     3421 agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg ggccgcgggc
     3481 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     3541 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     3601 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     3661 tgagcggaaa gatggccgct tcccggccct gctccagggg gctcaaaatg gaggacgcgg
     3721 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaggggcctt tccgtcctca
     3781 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     3841 tggagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     3901 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     3961 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     4021 caaagttttt ttcttccatt tcaggtgtcg tgaacacgct agcgccacca tgggggcagg
     4081 tgccactggc cgcgccatgg acgggccgcg cctgctgctg ttgctgcttc tgggggtgtc
     4141 ccttggaggt gccaaggagg catgccccac aggcctgtac acacacagcg gtgagtgctg
     4201 caaagcctgc aacctgggcg agggtgtggc ccagccttgt ggagccaacc agaccgtgtg
     4261 tgagccctgc ctggacagcg tgacgttctc cgacgtggtg agcgcgaccg agccgtgcaa
     4321 gccgtgcacc gagtgcgtgg ggctccagag catgtcggcg ccatgcgtgg aggccgacga
     4381 cgccgtgtgc cgctgcgcct acggctacta ccaggatgag acgactgggc gctgcgaggc
     4441 gtgccgcgtg tgcgaggcgg gctcgggcct cgtgttctcc tgccaggaca agcagaacac
     4501 cgtgtgcgag gagtgccccg acggcacgta ttccgacgag gccaaccacg tggacccgtg
     4561 cctgccctgc accgtgtgcg aggacaccga gcgccagctc cgcgagtgca cacgctgggc
     4621 cgacgccgag tgcgaggaga tccctggccg ttggattaca cggtccacac ccccagaggg
     4681 ctcggacagc acagccccca gcacccagga gcctgaggca cctccagaac aagacctcat
     4741 agccagcacg gtggcaggtg tggtgaccac agtgatgggc agctcccagc ccgtggtgac
     4801 ccgaggcacc accgacaacc tcatccctgt ctattgctcc atcctggctg ctgtggttgt
     4861 gggtcttgtg gcctacatag ccttcaagag gtggaacagc gggtctggcg ccacaaactt
     4921 ctctctgcta aagcaagcag gtgatgttga agaaaacccc gggcctaccg agtacaagcc
     4981 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     5041 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     5101 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     5161 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5221 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     5281 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     5341 caccgtcgga gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     5401 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     5461 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     5521 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgactcgagt aaggatccta
     5581 atctagataa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     5641 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     5701 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     5761 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     5821 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5881 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5941 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     6001 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6061 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6121 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     6181 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6241 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     6301 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6361 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6421 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6481 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     6541 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6601 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6661 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6721 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     6781 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6841 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6901 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6961 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7021 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7081 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7141 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     7201 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     7261 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     7321 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7381 tcagcaacca tagtcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7441 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     7501 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     7561 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
     7621 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
     7681 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
     7741 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
     7801 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
     7861 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
     7921 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
     7981 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
     8041 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     8101 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     8161 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8221 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8281 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     8341 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8401 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8461 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8521 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8581 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8641 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8701 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8761 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8821 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8881 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8941 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9001 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9061 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9121 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     9181 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9241 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9301 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9361 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9421 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9481 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9541 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9601 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9661 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9721 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9781 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9841 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9901 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9961 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10021 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10081 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10141 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10201 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10261 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10321 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10381 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10441 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    10501 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
    10561 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
    10621 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
    10681 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
    10741 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
    10801 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
    10861 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
    10921 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
    10981 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
    11041 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
    11101 gg
//
