LOCUS       sequence-394511-        4695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains PER5 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP48
FEATURES             Location/Qualifiers
     source          1..4695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4281..4300)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4282..4300)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4310..4332)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4526..4545)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4645..4667
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tcagtgcgta gtagtgagtt ttcttcattt
     2281 tttctaaaga attatataaa cgaatatatt aatatttcaa tttgaccggt aatcttaatc
     2341 aatatataaa cacacgtcat ttggtgtgaa tttctaatgg aggacagctt ttagtctaat
     2401 tagatcatgt aagacagttc catacatgca ctgatctagt aacatattcg aacatagttg
     2461 aattgacctt ttcatcaaag gaaaatgaaa cttactaaac acatatatat actcacccat
     2521 atgggcagac gacctagttt gaagaagagc tagatgaaaa tgacacccct aaaataggaa
     2581 agtaaatata tataattttt ttttgacaaa aatgtttgaa aacatatttg gtttagtacg
     2641 tacgaccccg ttaagtatta tggtttggac ttcgaaatct ccattatgtt atgctttggt
     2701 cacgatatca aaaaaaaata ttattattat aataaatcta ggcctgccat catatggatt
     2761 aatagatatt agccaattca tatgcataca tgtatatatt atgtaccatg atactttttc
     2821 ttttttggtt gatatgctac tattatattt ttttcgtagt tgagcggtgt ctacccataa
     2881 acataactgt ttcaaactat ttgtaaattg taatcataaa acttcgaaat tctataggcg
     2941 agatgttaga agattttgtt aaatattata gaagaaattc tcttagctaa tttttccctt
     3001 ttttcgaact gtggaactat ttgaatgctc atctagttgt atatatgcta aaagatgggg
     3061 gttttccaat cgttcgaaca taaatacata atgaatcacc acgtactaaa atcaactaag
     3121 gaaacaaatg aaattacagg tatttttgtg cgtaaatcat ttaagagatg aatgattcga
     3181 atttcgattc ttagggaaaa acacaactac taccgtacta cgtatgagat gcaaaacact
     3241 acaaaaaagg ttaaacacga ctacgcacaa taatgactgt ccatagcgat atgccgatac
     3301 atcgaaattc ctaacaaatt tatcattatt ataaatcaaa ctactgacga atcaaatttg
     3361 aggagtggat taataaataa cttttaatgt tatcggtgtt agcccgcaaa gactggatta
     3421 tgcgattttt ttttaaagga aagaaaagcg acagtacata gctagcttca tatatgatcg
     3481 ccattgacca tgcattgttt tctttcatat atatctctaa gaattgtcat cgtaataggg
     3541 acattgcaag tcctctagtt actaaataat cactcttttt atttttcaca atatatagct
     3601 caattccaat tttatttgct aaaaaatacc acatcgataa aaatttgtcc accaaccaaa
     3661 ttcataacgt gtttcaaacg aatcattcac tcgttctgag acgaatatat ataattagct
     3721 actaaattaa attaaaaact attttttgtt tggttaatgt gttatatttt gactttctaa
     3781 ttatatgttc gtcaactatt cctttcaaac taatcgcaca aaataattgt tcgttatatt
     3841 cttcagatcc gaacttcaac ctgtattatt ataagaataa actatttaag cgcttactaa
     3901 tgatgttctt gtttttcttc tcaaactttt aggttatctg tttttataag ttaacaattt
     3961 gacaattgac caaaatacaa agtcagagcc caacaattaa tttattttaa tccaaatatt
     4021 ctctagctaa catatgatag atctaacata ttttaaatta cagtctccaa ccttgaacag
     4081 cgacaccgcg tgaaaactct catgcacgta tattatataa acccctaggg tttgtaacat
     4141 tcaacatcaa tctcaaataa ttcatcaacg agaaaagatt aattcttact accaaaagaa
     4201 agaagataaa ttactatttt gttttacgta cgaaatatat accaagtgag atctacaaat
     4261 aacatgagac cgaattctcg ccctatagtg agtcgtatta cctggccgtc gttttacaac
     4321 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     4381 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     4441 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     4501 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     4561 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4621 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     4681 caccgaaacg cgcga
//
