LOCUS       sequence-394988-        1326 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SPAX8-related truncated mutation (E189*) containing the amino acids 
            1 to 188 of human NKX6-2 fused with the Venus fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Ferreira-Peralta P, Franca B, Murtinheira F, Rodrigues MS, Herrera F
  TITLE     Is spastic ataxia 8 a protein misfolding disorder?
  JOURNAL   Biochim Biophys Acta Mol Basis Dis. 2023 Sep 9;1870(1):166882. doi: 
            10.1016/j.bbadis.2023.166882.
  PUBMED    37696463
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta Mol Basis Dis."; date: "2023-09-9"; volume: 
            "1870(1):166882. doi"; pages: " 10.1016/j.bbadis.2023.166882"
COMMENT     Sequence Label: pcDNA3.1 NKX6-2(1-188)-Venus
FEATURES             Location/Qualifiers
     source          1..1326
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(913..932)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggacacaa acagacctgg cgcctttgtg ctgtctagcg ctcctctggc cgctctgcac
       61 aatatggccg agatgaagac cagcctgttt ccatacgctc tgcagggacc tgccggcttt
      121 aaggctcctg ctcttggagg actgggagca caactgcctc tgggaacacc tcacggcatc
      181 agcgatattc tgggcagacc tgttggagct gctggcggag gattgctcgg aggactgcct
      241 agactgaatg gactggcttc ttctgccggc gtgtactttg gacctgctgc tgccgttgcc
      301 agaggctatc ctaaacctct ggctgagctt cccggcagac ctcctatttt ttggcctggt
      361 gttgtgcagg gcgctccttg gagagatcct agacttgctg gaccagctcc tgcaggcgga
      421 gtgctggata aggatggcaa gaagaagcac agcagaccca ccttcagcgg ccagcagatc
      481 tttgccctgg aaaagacctt cgagcagacc aagtatctgg ctggccctga gagagccaga
      541 ctggcctatt ctctgggcat gaccaagctt ggcggaggtg gaagcggcgg aggcggatct
      601 ggtaccatgg tttctaaggg cgaagaactg ttcaccggcg tggtgcctat cctggtggaa
      661 ctggatgggg atgtgaacgg ccacaagttc tctgtgtctg gcgaaggcga aggggatgcc
      721 acatacggca agctgaccct gaagctgatc tgcaccacag gcaaactgcc cgtgccttgg
      781 cctacactgg tcacaacact cggctacggc ctgcagtgct ttgccagata tcccgaccac
      841 atgaagcagc acgatttctt caagagcgcc atgcctgagg gctacgtgca agagcggacc
      901 atcttcttca aggacgacgg caactacaag accagggccg aagtgaagtt cgagggcgac
      961 accctggtca acagaatcga gctgaagggc atcgacttta aagaggacgg caacatcctg
     1021 ggccacaagc tcgagtacaa ctacaacagc cacaacgtgt acatcaccgc cgacaagcag
     1081 aagaacggca tcaaggccaa cttcaagatc cggcacaaca tcgaggatgg cggcgtgcag
     1141 ctggccgatc actatcagca gaacacccct atcggagatg gccccgtcct gctgcctgat
     1201 aaccactacc tgagctacca gtctgccctg agcaaggacc ccaatgagaa gagggatcac
     1261 atggtgctgc tggaattcgt gacagccgcc ggaatcacac tcggcatgga cgagctgtac
     1321 aaatga
//
