LOCUS       sequence-395096-        1200 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for ectopic integration and expression of Khd4-Ada-Gfp. The 
            4282 bp khd4-ORF is fused C-terminally to the 1200 bp adar catalytic
            domain (Ada) with three repeats of GGGGS linker in between, fol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Sankaranarayanan S, Haag C, Petzsch P, Kohrer K, Matuszynska A, 
            Zarnack K, Feldbrugge M
  TITLE     The mRNA stability factor Khd4 defines a specific mRNA regulon for 
            membrane trafficking in the pathogen Ustilago maydis.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Aug 22;120(34):e2301731120. doi: 
            10.1073/pnas.2301731120. Epub 2023 Aug 17.
  PUBMED    37590419
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-08-22"; volume: "120(34):e2301731120. 
            doi"; pages: " 10.1073/pnas.2301731120. Epub 2023 Aug 17"
COMMENT     Sequence Label: p Pcrg1: Khd4-Ada-Gfp (CbxR)
FEATURES             Location/Qualifiers
     source          1..1200
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggtggttc ctcaaaagaa tgtgccactg cctatcgatg ataagtcgtc ttctatggaa
       61 ctgcctcaga tccacgcaga taccattggc cgactcgtcc tcgaaaagtt catggaagtg
      121 attaagggac aggaagcgta ttcccgacgc aaggtgctgg ctggtatcgt catgaccgag
      181 aacatgaatt tttgtgaggc taaggtgatc tctgtctcga ccggcaccaa atgtgtctcc
      241 ggagagcaca tgtcggtcaa tggagcagtg ttgaatgatt cccatgcaga aattgtttcc
      301 cgtcgttgtt tgctgaaata tctctacgct caactggacc tgcaatgcaa tcaggcaaca
      361 gcttaccaat cgatcttcgt tcgtaacaca gatggacagt atccatacaa gctcaagagc
      421 ggtgtgcatt tccatctgta catcaacaca gcgccgtgcg gcgacgcccg aattttttcg
      481 ccccacgaaa atgataccgg cgttgataag catccaaatc gtaaggcgcg aggccaattg
      541 cgaacaaaga tcgaatcggg acaaggcaca atcccggtga agagcagcga tggcattcag
      601 acctgggacg gagttctcca aggacagcgt ctgcttacca tgtcgtgctc tgataaaatt
      661 gctcgctgga atattgtcgg catccaggga tcgctgctgt cgtccatcat cgagccggtg
      721 tacctccatt ctatcgttct gggatcgttg ctgcaccctg agcacatgta ccgtgccgtg
      781 tgtggccgta tcgagaagtc gatccaggga ctccctccac cttaccacct gaataaacca
      841 cgacttgctc tcgtcacctc ggccgaaccg cgcaatcagg cgaaagcgcc taactttggc
      901 attaactgga ccattggaga caccgaactt gaagtggtga actctctgac tggtcgcacc
      961 attggcggcc aggtttcgcg catcacgaag caagcctttt ttgtcaaata tggcttcctg
     1021 atggcgaacc tgcccggtat cctcgtccgt aaggttacga cagactatgg ccagacgaaa
     1081 gcgaacgtga aagattatca aattgccaag ctggaactgt tttcggcctt taaacgcgag
     1141 gatttgggat cgtggctcaa aaagccgatc gaacaagatg agttcggact ggcagaatcc
//
