LOCUS       sequence-395098-         310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for ectopic integration and expression of Khd4-Ada-Gfp. The 
            4282 bp khd4-ORF is fused C-terminally to the 1200 bp adar catalytic
            domain (Ada) with three repeats of GGGGS linker in between, fol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Sankaranarayanan S, Haag C, Petzsch P, Kohrer K, Matuszynska A, 
            Zarnack K, Feldbrugge M
  TITLE     The mRNA stability factor Khd4 defines a specific mRNA regulon for 
            membrane trafficking in the pathogen Ustilago maydis.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Aug 22;120(34):e2301731120. doi: 
            10.1073/pnas.2301731120. Epub 2023 Aug 17.
  PUBMED    37590419
REFERENCE   2  (bases 1 to 310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-08-22"; volume: "120(34):e2301731120. 
            doi"; pages: " 10.1073/pnas.2301731120. Epub 2023 Aug 17"
COMMENT     Sequence Label: p Pcrg1: Khd4-Ada-Gfp (CbxR)
FEATURES             Location/Qualifiers
     source          1..310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      27..279
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 agcggccggc gcgccggccg cccggcgatc gttcaaacat ttggcaataa agtttcttaa
       61 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
      121 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
      181 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
      241 ataaattatc gcgcgcggtg tcatctatgt tactagatcc gatgataagc tgtcaaacat
      301 gagaattctt
//
