LOCUS       sequence-395125-       10090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The template UPEC vector designed for cloning of a single pegRNA and
            subsequent co-expression with Cre..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Ely ZA, Mathey-Andrews N, Naranjo S, Gould SI, Mercer KL, Newby GA, 
            Cabana CM, Rideout WM 3rd, Jaramillo GC, Khirallah JM, Holland K, 
            Randolph PB, Freed-Pastor WA, Davis JR, Kulstad Z, Westcott PMK, Lin
            L, Anzalone AV, Horton BL, Pattada NB, Shanahan SL, Ye Z, Spranger 
            S, Xu Q, Sanchez-Rivera FJ, Liu DR, Jacks T
  TITLE     A prime editor mouse to model a broad spectrum of somatic mutations 
            in vivo.
  JOURNAL   Nat Biotechnol. 2023 May 11. doi: 10.1038/s41587-023-01783-y.
  PUBMED    37169967
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 May 11. doi: 10.1038/s41587-023-01783-y."
COMMENT     Sequence Label: hU6-pegRNA-EF-1-alpha-Cre Template
FEATURES             Location/Qualifiers
     source          1..10090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        46..294
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     46..66
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     217..236
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     terminator      complement(304..335)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(352..1029)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             1036..1047
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    1065..1083
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1090..1108
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        1178..1389
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             1440..1460
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1461..2489
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    2575..3163
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2628..2648)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3046..3057)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3235..3468
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(3494..3511)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3500..3724
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3770..4198
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3857..3876)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4067..4088
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4207..4227)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4212..4541
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4392..4527
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4454..4473
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4589..4636
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4655..5029
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5159..5280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5196..5215)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5250..5269
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5329..5345)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5329..5345)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5342..5364)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5353..5369
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5377..5383,5384..5401,5402..5407))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5422..5443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5560..5577)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5731..6319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5811..5830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6490..7281,7282..7350))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7113..7132
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7351..7455)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7530..7549)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7721..8100
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8102..8300
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8255..8275
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8318..8498
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8545..8670
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9163..9396
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9581..9625
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9923..10040
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gatcagtgtg agggagtgta aagctggttt aaggtcgggc aggaagaggg cctatttccc
       61 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
      121 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
      181 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
      241 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa cacccgagac
      301 ggtagtcaaa agcctccggt cggaggcttt tgacttggct gaggagtgcc attaagcacc
      361 ggtggagtga cgaccttcag cacgttcgta ctgttcaacg atggtgtagt cttcgttgtg
      421 ggaggtgatg tccagtttga tgtcggtttt gtaagcaccc ggcagctgaa ccggtttttt
      481 agccatgtag gtggttttaa cttcagcgtc gtagtgacca ccgtctttca gtttcagacg
      541 cattttgatt tcacctttca gagcaccgtc ttccgggtac atacgttcgg tggaagcttc
      601 ccaacccatg gtttttttct gcataaccgg accgtcggac gggaagttgg taccacgcag
      661 tttaactttg tagatgaact caccgtcttg cagggaggag tcctgggtaa cggtaacaac
      721 accaccgtct tcgaagttca taacacgttc ccatttgaaa ccttccggga aggacagttt
      781 caggtagtcc gggatgtcag ccgggtgttt aacgtaagct ttggaaccgt actggaactg
      841 cggggacagg atgtcccaag cgaacggcag cggaccacct ttggtaactt tcagtttagc
      901 ggtctgggta ccttcgtacg gacgaccttc accttcacct tcgatttcga actcgtgacc
      961 gttaacggaa ccttccatac gaactttgaa acgcatgaac tctttgataa cgtcttcgct
     1021 actcgccata gatcctttct cctctttcag atccgtgctc agtatctcta tcactgatag
     1081 ggatgtcaat ctctatcact gatagggacg tctcgttttt ttaaaccgct gttcctagga
     1141 atcccgaggc cttcgagtgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     1201 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     1261 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     1321 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     1381 agaacacagg tgtcgtgacg cgggacccga cattagcgct acagcttaag cggaccatgc
     1441 ccaagaagaa gaggaaggtg tccaatttac tgaccgtaca ccaaaatttg cctgcattac
     1501 cggtcgatgc aacgagtgat gaggttcgca agaacctgat ggacatgttc agggatcgcc
     1561 aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat
     1621 ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc
     1681 ttctatatct tcaggcgcgc ggtctggcag taaaaactat ccagcaacat ttgggccagc
     1741 taaacatgct tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat gctgtttcac
     1801 tggttatgcg gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc
     1861 tagcgttcga acgcactgat ttcgaccagg ttcgttcact catggaaaat agcgatcgct
     1921 gccaggatat acgtaatctg gcatttctgg ggattgctta taacaccctg ttacgtatag
     1981 ccgaaattgc caggatcagg gttaaagata tctcacgtac tgacggtggg agaatgttaa
     2041 tccatattgg cagaacgaaa acgctggtta gcaccgcagg tgtagagaag gcacttagcc
     2101 tgggggtaac taaactggtc gagcgatgga tttccgtatc tggtgtagct gatgatccga
     2161 ataactacct gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc
     2221 agctatcaac tcgcgccctg gaagggattt ttgaagcaac tcatcgattg atttacggcg
     2281 ctaaggatga ctctggtcag agatacctgg cctggtctgg acacagtgcc cgtgtcggag
     2341 ccgcgcgaga tatggcccgc gctggagttt caataccgga gatcatgcaa gctggtggct
     2401 ggaccaatgt aaatattgtc atgaactata tccgtaacct ggatagtgaa acaggggcaa
     2461 tggtgcgcct gctggaagat ggcgattaga aacgttgttg tttggggttg aattactcta
     2521 catgtttaag ggttccggtt ccactaggta caattcgata tcaagcttat cgataatcaa
     2581 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     2641 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     2701 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     2761 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     2821 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     2881 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     2941 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3001 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3061 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3121 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     3181 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     3241 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     3301 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3361 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3421 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccggcgaa
     3481 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3541 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3601 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3661 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     3721 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     3781 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3841 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3901 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     3961 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4021 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4081 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4141 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     4201 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4261 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4321 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4381 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4441 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     4501 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     4561 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     4621 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     4681 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     4741 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     4801 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     4861 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     4921 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     4981 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     5041 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5101 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     5161 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5221 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5281 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     5341 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5401 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5461 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5521 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5581 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5641 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5701 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5761 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5821 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5881 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5941 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6001 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6061 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6121 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6181 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6241 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6301 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6361 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6421 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6481 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6541 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6601 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6661 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6721 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6781 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6841 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6901 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6961 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7021 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7081 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7141 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7201 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7261 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7321 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7381 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7441 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     7501 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     7561 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     7621 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     7681 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     7741 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     7801 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     7861 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     7921 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     7981 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     8041 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     8101 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     8161 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     8221 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     8281 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
     8341 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8401 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8461 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     8521 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     8581 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     8641 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     8701 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     8761 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     8821 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     8881 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     8941 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9001 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     9061 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     9121 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     9181 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     9241 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     9301 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     9361 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     9421 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     9481 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     9541 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     9601 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     9661 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     9721 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     9781 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     9841 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     9901 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     9961 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    10021 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa
    10081 ttaaggtacc
//
