LOCUS       sequence-39527-e        4245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA, Guglielmi KM, 
            Holm GH, Johnson EM, Maginnis MS, Naik S, Skelton WB, Wetzel JD, 
            Wilson GJ, Chappell JD, Dermody TS
  TITLE     A plasmid-based reverse genetics system for animal double-stranded 
            RNA viruses.
  JOURNAL   Cell Host Microbe. 2007 Apr 19;1(2):147-57.
  PUBMED    18005692
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe."; date: "2007-04-19"; volume: "1(2)"; pages: "147-57"
COMMENT     Sequence Label: pT7-S4T1L
FEATURES             Location/Qualifiers
     source          1..4245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1361..1379)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1375..1422
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1490..1508)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1491..1508)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1799..1818)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2029..2048)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2148..2170
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2208..2226)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2294..2398
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2399..2467,2468..3259)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2617..2636)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3430..4018
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3919..3938
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4172..4189
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttaatacgac tcactatagc tatttttgcc tcttcccaaa cgttgtcgca atggaggtgt
       61 gcttgcccaa cggtcatcag atcgtggact tgattaacaa cgcttttgaa ggtcgtgtat
      121 caatctacag cgcgcaagag ggatgggaca aaacaatctc agcacagcca gatatgatgg
      181 tatgtggtgg tgccatcgtt tgcatgcatt gtctaggtgt tgttggatct ctgcaacgca
      241 agctgaagca tttgcctcac catagatgta atcaacagat tcgtcatcag gattacgtcg
      301 atgtacagtt cgcagatcgt gttactgctc actggaagcg gggtatgctg tcctttgttg
      361 cgcagatgca cgcgatgatg aatgacgtat caccagagga tctagaccgt gtgcgtactg
      421 agggaggttc actagtggag ttaaactggc ttcaggttga tccaaattca atgtttagat
      481 caatacactc aagttggaca gatcctctgc aggtagtgga tgatcttgac actaagctgg
      541 atcaatactg gacggccctg aatctgatga ttgattcatc cgacttggtg cccaacttca
      601 tgatgagaga cccatcacat gcattcaatg gtgtgagact ggagggagac gcccgccaaa
      661 ctcaattctc taggactttc gattcgagat cgagtttaga atggggtgtg atggtttacg
      721 attactctga gttagagcat gatccatcga agggccgtgc ttacaggaag gaattggtga
      781 cgccagcacg agacttcggt cattttggat tatcccacta ctctagggcg actaccccaa
      841 tccttggaaa gatgccagct gtattctcgg gaatgttgac tgggaattgt aaaatgtatc
      901 cattcatcaa aggaacggct aagttaaaga cagtgcgcaa gctagtggat tcagtcaatc
      961 atgcgtgggg cgtcgagaag atcagatatg cgcttggacc aggtggcatg acgggatggt
     1021 acaacaggac tatgcagcag gcccccattg tactaactcc cgctgctctc acaatgttct
     1081 cagacaccac caagttcggg gatttggatt atccggtgat gattggcgat ccgatgattc
     1141 ttggctaaac acccccatct tcacagcgcc gggcctgacc aacctggtgt gacgtgggac
     1201 aggctccatt catcgggtcg gcatggcatc tccacctcct cgcggtccga cctgggcatc
     1261 cgaaggagga cgtcgtccac tcggatggct aagggagagc tcaaaaaaaa ggatccggct
     1321 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     1381 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     1441 tccggatcga gatcctctag agtcgacctg caggcatgca agcttgtatt ctatagtgtc
     1501 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg
     1561 acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc
     1621 tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg
     1681 ccgttccgca ccccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     1741 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     1801 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     1861 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     1921 ttgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     1981 taatgcagga gtcgcataag ggagagcgtc gatatggtgc actctcagta caatctgctc
     2041 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     2101 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     2161 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     2221 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2281 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2341 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2401 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2461 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2521 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2581 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2641 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2701 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2761 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2821 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2881 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2941 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3001 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3061 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3121 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3181 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3241 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3301 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3361 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3421 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3481 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3541 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     3601 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3661 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3721 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3781 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3841 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3901 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3961 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4021 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4081 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4141 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4201 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attca
//
