LOCUS       sequence-395304-        6062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains CORK1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP09
FEATURES             Location/Qualifiers
     source          1..6062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5648..5667)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5649..5667)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5677..5699)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5893..5912)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6012..6034
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgtacactta ttaacatata tttttaattt
     2281 tgtgaattaa agtagacaag tccgattcaa aagaaaaaaa gtccagtcat aaatatattt
     2341 atttcttggt ttcgtggaaa agaggtcgta gcgctctgca tttgccgtgg acaataggaa
     2401 aagttgtgaa agagatgttg cgtggaaatg tatggtataa ttcataaatt taaatagata
     2461 aatccagtct gaaatgtggt tatatatttc aacggaacat aaaagaatta ggaaaagaga
     2521 ggcgtaaatt gcgggggacg tacttggagt atacgaagca gggacagtac aaaaatatgt
     2581 acaattccgt gactccgaat gtctcgtgca gtgtcaattt gtcactattc ttttctctct
     2641 ttttacagat ttttagaaac catgcatgat taatgttctt ttaaaaagtt gttgcatggt
     2701 tgtttttatt attgcaaacg tagtagaagg aagaacagtg tcaagtctat tagtgcatgc
     2761 ggttatatat aatccaaatg ataaatacgg atatagtact gctttctaaa gatgagttag
     2821 tgaaattaat gaattttggg ggaaataaac gccgtactat ggtccattcg tacagaatga
     2881 cccaccaact gactttatat ttcttccatt tacgtgtcat accaataata ccaacacgac
     2941 ttttcttcta aaattaaatg catgtatctg ttctttgaaa tgatgatttt cttattaggt
     3001 actgcataga gaacaccact atttgcttca tagttcatac aacgttttaa agtgaagttt
     3061 agtttggtaa aagttgcatt tacatactca ataatttaac agacgttgtg ctacttgcga
     3121 gtaatcacta ctggtcaatt aatatataac tcttccggag ttactcaaat agtgtgtata
     3181 gatatatagc aaattagcaa tggtttaaat gcgaaagcgt gggctgcccc aactgtccag
     3241 ccgtacgctc catgctacgg acactttgct ttcaacttcg acatttatgt ttcttattta
     3301 ataccatgag aattttggat atgagtttgt cctcacacat caaataaaaa tcatttagac
     3361 aatcatacaa ccaaatctta tctaactgaa tcaatttgaa tacttgatcc taaaacattg
     3421 ttagctcgaa gtctctagtt aagttccatc attgttccat aaacacaatc ttgaaatcaa
     3481 acatttcacc acggtgatct ctaaaagtag accactagtt tgtgcctcgc tcaaggccag
     3541 tgaaatatct gttagttatg tagtcaaaat ttcagtcgga ctttgataat aatagccaag
     3601 agagattgtc aagccagaat tttataatga ataactaaat aatatgagaa gatttagttg
     3661 ttcgttacag acagctctct gcactaacag ttctatttaa ctgacttgaa caacgaagaa
     3721 gacagattct taatgagttc ttgggcctta attattagta atcgattaaa aaaaaaatag
     3781 aatttctact gtgaaaattt caatttatga gtttagtatc atcgatttca tattggaaca
     3841 ttcatatgtt tgtcgagtga aatttttaaa agaaaataag actccgaaat attgagatca
     3901 ctgatacaag aaattaaatg tcctcatcaa gttaagagaa accaagagaa aaacctagag
     3961 attcaacaaa gttttccaac ctgacactga taggtcatga attgggcttg tgggctttat
     4021 attgtttaaa gcctgttatt cttagtattg ctttctattt aagtttgttg tcttgcttgg
     4081 taaacgttta tcgattaaga actcaagaat gtttatcttc ttcatcatct cctttcttct
     4141 tcctttcctt ctctgatttc ttcagcatct caacctattt cttctattat ctcatctcct
     4201 ttctgttact aaaattgata agcaccatta atttatatga acgtttacat aataattata
     4261 acatatattt atacttaacc ttatggatgt gcacaattat caatgttgta ttcacagctc
     4321 aaaaccctat ttgaccattc tttcgacaaa attggctatg cagaattgtt tttaggtgta
     4381 aggttttagc tttgtttttt ggatgtataa ggaggctaat tttttatttt ttttacggta
     4441 tattataatt tatgacattg tacggtttca tagtttattc acatacataa ttagtaataa
     4501 aaaatgtttt actaaaaatg atattcaaac caaaacatct aaacgtttat tttggaaatc
     4561 ggtcatttta tactcgacct aaatttaagt ggtcaattca tacaatattt ttcttttctt
     4621 atgtgctaat atattcaaat tatatatcca aatttaccaa atcatacaag attatacatt
     4681 ctagttcatt ctactgatgt agatgccaca tttttgatta ttttgatgac gtagttacca
     4741 cgctcttgac gatgtccgtg tcacgtcctc atcaccttat ttttttttgt ttcttcacct
     4801 ttttcttctt atatttttta ttttttgtct tctttatcac tattgctcga ggaagctgta
     4861 aaaatattat gtgtttgatg catgtaaaaa gactagaaaa attattttcg atactaatga
     4921 gtaataacca ttatttaaat gaaagaacaa aaatctaata gagtttatag tcaattcatc
     4981 aagtcattca gataaacatt gtattattgt tttcgttaga aaagtagtaa cggcaagaat
     5041 gtttttaaaa catggtcaaa cctattaact tgtatttgac gtccttgtcc gaatcataac
     5101 tctatcgcga aatggtcgtt tagtattagc aattggattg catcagacgt atgtatcttt
     5161 gcgaatattt tatacaaagt ggaagaaaag aaacacgtat gtattttggt ttcaaaatga
     5221 gacaactggc tgatgtcatt gatatgtcat atacggttcg attaatcaac tctatttaac
     5281 ccggttccac caaatcagca gtttaaaatt gaaattcgga aataacactt gatcacctaa
     5341 ttcattagtt taatactaat gattaacaag taaacaacga ctaatgataa aacggccagg
     5401 gctttccgac acactttttt tgttttattc atttattgga taactaaata caggaaaaag
     5461 cttacccaca tggcgacatt atttatcaaa aaaaagtaat taggctgtta aattctaaag
     5521 ttaaaaccat ataatataca tttatttata cgcataacaa gaacaaaaat ggctagttgg
     5581 tttactctgg atccctcccg actctctcac atctttggtc gtcgacgaac atgagaccga
     5641 attctcgccc tatagtgagt cgtattacct ggccgtcgtt ttacaacgtc gtgactggga
     5701 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5761 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     5821 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     5881 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     5941 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     6001 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     6061 ga
//
