LOCUS       sequence-395329-       11731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs 
            targeting miRNA302/367. Includes PGK-puro selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Sokka J, Yoshihara M, Kvist J, Laiho L, Warren A, Stadelmann C, 
            Jouhilahti EM, Kilpinen H, Balboa D, Katayama S, Kyttala A, Kere J, 
            Otonkoski T, Weltner J, Trokovic R
  TITLE     CRISPR activation enables high-fidelity reprogramming into human 
            pluripotent stem cells.
  JOURNAL   Stem Cell Reports. 2022 Feb 8;17(2):413-426. doi: 
            10.1016/j.stemcr.2021.12.017. Epub 2022 Jan 20.
  PUBMED    35063129
REFERENCE   2  (bases 1 to 11731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2021.12.017"; journalName: "Stem Cell Reports"; 
            date: "2022-02-8- 8"; volume: "17"; issue: "2"; pages: "413-426"
COMMENT     Sequence Label: GG-EBNA-MIR302-7guides-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..11731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     134..153
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        134..152
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     219..235
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        236..484
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        506..581
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     613..629
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..878
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        900..975
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(987..1003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1007..1023
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1024..1272
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1294..1369
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1401..1417
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1418..1666
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1688..1763
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1795..1811
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1812..2060
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2082..2157
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     2189..2205
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2206..2454
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2476..2551
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     2583..2599
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2600..2848
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2870..2945
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    3103..3136
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3197..3696
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3232..3255)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3629..3648
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3736..4335
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3736..3755)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             4784..6709
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGXGGXGXGGGXGXGGGXGGXGGXGXGGGXGXGGGXGGXGXGGGX
                     GGXGGXGXGGGAGAGGGAGGXGXGGGXGGXGGAGAGGGAGAGGXGGXGGXGXGGXGXGG
                     GXGGXGGAGAGGAGAGGAGAGGAGXGGAGGXGXGGXGGXGXGGXGGXGXGGGXGGXGXG
                     GGXGGXGXGGXGGXGXGGXGGXGXGGAGGXGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    6877..6910
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      7097..8886
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(9455..9474)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9574..9596
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9634..9652)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9720..9824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9825..9893,9894..10685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10043..10062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10856..11444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11345..11364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag ctctaatacg actcactata gggagacaag cttgcatgcc tgcaggtcga
      181 ctctagagga tctcgagctc aagcttcgaa ttccccctgt aaaacgacgg ccagtgaggg
      241 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
      301 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
      361 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
      421 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
      481 caccgaagaa tagtataaat agaaggtttt agagctagaa atagcaagtt aaaataaggc
      541 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttca tggtcatagc
      601 tgtttcctca tggtaaaacg acggccagtg agggcctatt tcccatgatt ccttcatatt
      661 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
      721 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
      781 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
      841 tcttggcttt atatatcttg tggaaaggac gaaacaccga tctcagagaa tcattacaag
      901 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      961 gcaccgagtc ggtgcttttt ttcatggtca tagctgtttc ctggacgtaa aacgacggcc
     1021 agtgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     1081 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     1141 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     1201 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     1261 ggacgaaaca ccgagggaat gtatgatcct ggggttttag agctagaaat agcaagttaa
     1321 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttcatg
     1381 gtcatagctg tttcctccag gtaaaacgac ggccagtgag ggcctatttc ccatgattcc
     1441 ttcatatttg catatacgat acaaggctgt tagagagata attggaatta atttgactgt
     1501 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
     1561 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
     1621 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgaaa aggatccaga
     1681 cccacccgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     1741 aaaaagtggc accgagtcgg tgcttttttt catggtcata gctgtttcct tgttgtaaaa
     1801 cgacggccag tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     1861 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
     1921 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     1981 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2041 tgtggaaagg acgaaacacc gtttaagagg aagatatctt ggttttagag ctagaaatag
     2101 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     2161 ttttcatggt catagctgtt tccttgcagt aaaacgacgg ccagtgaggg cctatttccc
     2221 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
     2281 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     2341 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     2401 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgatgcc
     2461 atcaaacaag cagatgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     2521 tcaacttgaa aaagtggcac cgagtcggtg ctttttttca tggtcatagc tgtttcctcg
     2581 gtgtaaaacg acggccagtg agggcctatt tcccatgatt ccttcatatt tgcatatacg
     2641 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
     2701 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     2761 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     2821 atatatcttg tggaaaggac gaaacaccgc aatgcctttc tcggctcagg ttttagagct
     2881 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     2941 ggtgcttttt ttcatggtca tagctgtttc ctaacgagcg gccgcaggaa cccctagtga
     3001 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     3061 tcgcccgacg cccaattccg atcatattca ataaccctta atataacttc gtataatgta
     3121 tgctatacga agttattagg tctgaagagg agtttacgtc cagccaagct taggatctcg
     3181 acctcgaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     3241 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     3301 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     3361 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     3421 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     3481 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     3541 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     3601 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     3661 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatccatct agatctcgag
     3721 cagctgaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     3781 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     3841 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     3901 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     3961 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     4021 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     4081 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     4141 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     4201 gtgcccgcct tcctggagac ctccgcgccc cacaacctcc ccttctacga gcggctcggc
     4261 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     4321 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     4381 ccccatgcat cgataagctg atcctcacag gccgcaccca gcttttcttc cgttgcccca
     4441 gtagcatctc tgtctggtga ccttgaagag gaagaggagg ggtcccgaga atccccatcc
     4501 ctaccgtcca gcaaaaaggg ggacgaggaa tttgaggcct ggcttgaggc tcaggacgca
     4561 aatcttgagg atgttcagcg ggagttttcc gggctgcgag taattggtga tgaggacgag
     4621 gatggttcgg aggatgggga attttcagac ctggatctgt ctgacagcga ccatgaaggg
     4681 gatgagggtg ggggggctgt tggagggggc aggagtctgc actccctgta ttcactgagc
     4741 gtcgtctaat aaagatgtct attgatctct tttagtgtga atcatgtctg acgaggggcc
     4801 aggtacagga cctggaaatg gcctaggaga gaagggagac acatctggac cagaaggctc
     4861 cggcggcagt ggacctcaaa gaagaggggg tgataaccat ggacgaggac ggggaagagg
     4921 acgaggacga ggaggcggaa gaccaggagc cccgggcggc tcaggatcag ggccaagaca
     4981 tagagatggt gtccggagac cccaaaaacg tccaagttgc attggctgca aagggaccca
     5041 cggtggaaca ggagcaggag caggagcggg aggggcagga gcaggagggg caggagcagg
     5101 aggaggggca ggagcaggag gaggggcagg aggggcagga ggggsaggag gggsaggags
     5161 aggaggaggg gsaggagsag gaggaggggs aggaggrgsa ggaggrgsag gagsaggagg
     5221 aggggsagga gsaggaggag gggsaggagg ggsaggagsa ggaggagggg saggaggrgs
     5281 aggaggggsa ggagsaggag gaggggcagg agcaggagga ggggcaggag gggsaggags
     5341 aggaggaggg gsaggaggrg saggaggggc aggagcagga ggaggggcag gagcaggagg
     5401 ggsaggaggg gsaggagggg saggagsagg aggggsagga gsaggaggag gggsaggagg
     5461 ggsaggaggg gcaggagcag gaggggcagg agcaggaggg gcaggagcag gaggggcagg
     5521 agsaggaggg gcaggagggg saggagsagg aggggsagga ggggsaggag saggaggggs
     5581 aggaggggsa ggagsaggag gaggggsagg aggggsagga gsaggaggag gggsaggagg
     5641 ggsaggagsa ggaggggsag gaggggsagg agsaggaggg gsaggagggg saggagsagg
     5701 aggggcagga ggggsaggag caggaggagg ggcaggagca ggaggggcag gagcaggagg
     5761 tggaggccgg ggtcgaggag gcagtggagg ccggggtcga ggaggtagtg gaggccgggg
     5821 tcgaggaggt agtggaggcc gccggggtag aggacgtgaa agagccaggg ggggaagtcg
     5881 tgaaagagcc agggggagag gtcgtggacg tggagaaaag aggcccagga gtcccagtag
     5941 tcagtcatca tcatccgggt ctccaccgcg caggccccct ccaggtagaa ggccattttt
     6001 ccaccctgta ggggaagccg attattttga ataccaccaa gaaggtggcc cagatggtga
     6061 gcctgacgtg cccccgggag cgatagagca gggccccgca gatgacccag gagaaggccc
     6121 aagcactgga ccccggggtc agggtgatgg aggcaggcgc aaaaaaggag ggtggtttgg
     6181 aaagcatcgt ggtcaaggag gttccaaccc gaaatttgag aacattgcag aaggtttaag
     6241 agctctcctg gctaggagtc acgtagaaag gactaccgac gaaggaactt gggtcgccgg
     6301 tgtgttcgta tatggaggta gtaagacctc cctttacaac ctaaggcgag gaactgccct
     6361 tgctattcca caatgtcgtc ttacaccatt gagtcgtctc ccctttggaa tggcccctgg
     6421 acccggccca caacctggcc cgctaaggga gtccattgtc tgttatttca tggtcttttt
     6481 acaaactcat atatttgctg aggttttgaa ggatgcgatt aaggaccttg ttatgacaaa
     6541 gcccgctcct acctgcaata tcagggtgac tgtgtgcagc tttgacgatg gagtagattt
     6601 gcctccctgg tttccaccta tggtggaagg ggctgccgcg gagggtgatg acggagatga
     6661 cggagatgaa ggaggtgatg gagatgaggg tgaggaaggg caggagtgat gtaacttgtt
     6721 aggagacgcc ctcaatcgta ttaaaagccg tgtattcccc cgcactaaag aataaatccc
     6781 cagtagacat catgcgtgct gttggtgtat ttctggccat ctgtcttgtc accattttcg
     6841 tcctcccaac atggggcaat tgccggaacc cttaatataa cttcgtataa tgtatgctat
     6901 acgaagttat taggtccctc gaagaggttc actagcggat ctcaattggg catacccatg
     6961 ttgtcacgtc actcagctcc gcgctcaaca ccttctcgcg ttggaaaaca ttagcgacat
     7021 ttacctggtg agcaatcaga catgcgacgg ctttagcctg gcctccttaa attcacctaa
     7081 gaatgggagc aaccagcagg aaaaggacaa gcagcgaaaa ttcacgcccc cttgggaggt
     7141 ggcggcatat gcaaaggata gcactcccac tctactactg ggtatcatat gctgactgta
     7201 tatgcatgag gatagcatat gctacccgga tacagattag gatagcatat actacccaga
     7261 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
     7321 tataaattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
     7381 tatagattag gatagcctat gctacccaga tatagattag gatagcatat gctacccaga
     7441 tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta cccagatata
     7501 aattaggata gcatatacta ccctaatctc tattaggata gcatatgcta cccggataca
     7561 gattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
     7621 gattaggata gcctatgcta cccagatata aattaggata gcatatacta cccagatata
     7681 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
     7741 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca tggcaacatt
     7801 agcccaccgt gctctcagcg acctcgtgaa tatgaggacc aacaaccctg tgcttggcgc
     7861 tcaggcgcaa gtgtgtgtaa tttgtcctcc agatcgcagc aatcgcgccc ctatcttggc
     7921 ccgcccacct acttatgcag gtattccccg gggtgccatt agtggttttg tgggcaagtg
     7981 gtttgaccgc agtggttagc ggggttacaa tcagccaagt tattacaccc ttattttaca
     8041 gtccaaaacc gcagggcggc gtgtgggggc tgacgcgtgc ccccactcca caatttcaaa
     8101 aaaaagagtg gccacttgtc tttgtttatg ggccccattg gcgtggagcc ccgtttaatt
     8161 ttcgggggtg ttagagacaa ccagtggagt ccgctgctgt cggcgtccac tctctttccc
     8221 cttgttacaa atagagtgta acaacatggt tcacctgtct tggtccctgc ctgggacaca
     8281 tcttaataac cccagtatca tattgcacta ggattatgtg ttgcccatag ccataaattc
     8341 gtgtgagatg gacatccagt ctttacggct tgtccccacc ccatggattt ctattgttaa
     8401 agatattcag aatgtttcat tcctacacta gtatttattg cccaaggggt ttgtgagggt
     8461 tatattggtg tcatagcaca atgccaccac tgaacccccc gtccaaattt tattctgggg
     8521 gcgtcacctg aaaccttgtt ttcgagcacc tcacatacac cttactgttc acaactcagc
     8581 agttattcta ttagctaaac gaaggagaat gaagaagcag gcgaagattc aggagagttc
     8641 actgcccgct ccttgatctt cagccactgc ccttgtgact aaaatggttc actaccctcg
     8701 tggaatcctg accccatgta aataaaaccg tgacagctca tggggtggga gatatcgctg
     8761 ttccttagga cccttttact aaccctaatt cgatagcata tgcttcccgt tgggtaacat
     8821 atgctattga attagggtta gtctggatag tatatactac tacccgggaa gcatatgcta
     8881 cccgtttagg gttaacaagg gggccttata aacactattg ctaatgccct cttgagggtc
     8941 cgcttatcgg tagctacaca ggcccctctg attgacgttg gtgtagcctc ccgtagtctt
     9001 cctgggcccc tgggaggtac atgtccccca gcattggtgt aagagcttca gccaagagtt
     9061 acacataaag gcaatgttgt gttgcagtcc acagactgca aagtctgctc caggatgaaa
     9121 gccactcagt gttggcaaat gtgcacatcc atttataagg atgtcaacta cagtcagaga
     9181 acccctttgt gtttggtccc cccccgtgtc acatgtggaa cagggcccag ttggcaagtt
     9241 gtaccaacca actgaaggga ttacatgcac tgccccgcga agaaggggca gagatgtcgt
     9301 agtcaggttt agttcgtccg gggcggggat cgatcctcta gtaagggatt ttgccgattt
     9361 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     9421 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     9481 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     9541 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     9601 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     9661 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     9721 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9781 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9841 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9901 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9961 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    10021 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    10081 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10141 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10201 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10261 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10321 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10381 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10441 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10501 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    10561 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    10621 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10681 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10741 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10801 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    10861 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    10921 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    10981 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    11041 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    11101 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    11161 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    11221 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    11281 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    11341 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    11401 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    11461 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    11521 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    11581 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    11641 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    11701 actggaaagc gggcagtgag cgcaacgcaa t
//
