LOCUS       sequence-395341-        4827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains BPS1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP38
FEATURES             Location/Qualifiers
     source          1..4827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4413..4432)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4414..4432)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4442..4464)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4658..4677)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4777..4799
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc taaaaagaga gtcacttggg ttaagtgatt
     2281 tccacacgac cattatattt catttttttt tccggtatat tattgtctca cttacataat
     2341 attttcattc tttggctctt cgagtcttgg ccttacgaaa ttaacattct tcttctttgt
     2401 aaccttttgt caacgatcac attcacatgt cacttattag atattgtaat atgtaatgtt
     2461 tggaccgacg tcgaagcata agcaggtgat tggtcgatgg atcaaactta tcgctttgga
     2521 cgaaatggac cacaatgatt cttttttagc tcacgtgatc gtgtttccaa acaatttttt
     2581 atcataaatc atctcgatta tggatggacc tagttgttgg ttatcatctt gaaaatttcc
     2641 aagttgcaag aatttaataa accagtcgag tattaaaatt ataatcaaac atgggtaact
     2701 tgcttgtctc gttaacacaa gataagtgca cacttgtctc aaccgacgac cggaatctat
     2761 attcacgtgt aggaggaaaa cttagactca aggacaacaa ttttgcctaa ataaaaactg
     2821 tagaaagtaa gattatgaat acatatacac ttgtacaagt cattccgttt tatgatacat
     2881 tgctatatct taaagaaaaa ttaaaccatt ttgtgtacga ggagaaagag taaaatatgt
     2941 gtgtttgtat tgcgtacaca aacgtcaata tgtatacata catgcatctt tttgatgtag
     3001 ttaacaaaat aaattatacc aaggaaaatt tctatcacta acaaaaacaa attgtttgta
     3061 aagcatataa attgttgcac taaataaaat cataaaacgc tagttaatat gtcaacatgc
     3121 ggtttaatcg accatcagag atattttcat tcatttaaga taatatcaca tttttccaat
     3181 ggccacttag ctaaattact ctttgaaaag tcattataga aaatcattaa caaatacgaa
     3241 aaaaaaaaat accactaaaa tggttaatca ttaacaaata cgaaagatct gaaaattagg
     3301 agccgtagga tccgctggct ataattgact ttttcatcag atgtacaagt aggcctgagt
     3361 tgaacaaaca catcgtaacc gtacacgcat tgatctgtca tctaagttgt tccgtcacag
     3421 ctccgtaact gaaacataat ctccacctgt cactatcgtc taccaaatcc acgacaaaac
     3481 ctaaatacca cttattagtt ttctttagtg gtttattaat ctgcagaaat acggcaactt
     3541 ttgtggttag tcatataaga acaaccgcgt tacaaacttc tctcgtcttt cctggaaatt
     3601 tctgtccaca aatattagtc tttaattttc ggtatgaaca ctgacccttc cttgatctat
     3661 tgatctttgt cgcagtattt attaagattt tatctctatc agacggctct tttgattaat
     3721 atttttccga ctcttatttc ttatcttctt tgctcagaaa cttctctcag gcgaattcaa
     3781 gtttcatagt tttggtctag tttttgtcca ccgatctatc tctttaggtg agttttgttt
     3841 atttttttcg gctttatagt ttgtctacgc ttcatgttgt tgttgttatt ataatcttgt
     3901 gtttgaattg aggtctttga tctcgtttct ttctgaattt agatatgtgt tattcggatt
     3961 tcagatcttt taagatctgg gtttggcttt ttatacagaa gaaaatggtt ttgacttaaa
     4021 aaattagaga tagctcttac ctcttacgct tgagtcttgg atctgttgat tttaaagctt
     4081 tatagatctt accacagtta gagaattgtt gttgactttt gtatgatgag ttcaaatttg
     4141 tgaattgaag aacagatctg atgttgtttg tgtttttctt tatatgagag gtttcattac
     4201 ctttgttttt aagatctgtt gagaaaagat ccatacttta gaaaaaaatc caaacatttg
     4261 atcttttgta ttggatatct aatatggatc tagaatataa tcaaagttat aatgtttttg
     4321 tattatggtt tgtgacaatt gatgtgttat ctttcttctt ctttcttgtt tttttcagtt
     4381 gaaacttatc agaacatgag accgaattct cgccctatag tgagtcgtat tacctggccg
     4441 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4501 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4561 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     4621 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     4681 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4741 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4801 tttcaccgtc atcaccgaaa cgcgcga
//
