LOCUS       sequence-395356-        4774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains SULTR4;1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP67
FEATURES             Location/Qualifiers
     source          1..4774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4360..4379)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4361..4379)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4389..4411)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4605..4624)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4724..4746
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tatgatccat cacacgcctg cctcacatac
     2281 actgatatgt ttttcattta ctttctttgt ttgaccataa ctccgaagtt cctttgttaa
     2341 tctgtttggt aatttatggt aactgcctgt tgacttttgt atgtctactt tttttttttt
     2401 tttgctcata agtgttgtct cttatttaaa ccaaagattg taaagattac aaaagagtgg
     2461 aaacctccgc tttcagtctc cagacaaaaa caaaataaag ataaaagtcc aactaaatta
     2521 aagcccagtt gaagcaagaa acccttttag gaagtaattg atgtagtcca ccgccgattt
     2581 ggttagtcat tgcagggaac tccaaaacca ccaggaacca ccaagacttc aagcttgtcc
     2641 gatggagcaa cacaaaattc agagaatccc atcaatcttc ttcatcttcg aattcgcaaa
     2701 ggaaaatctc ccctgggtag tcatatcatc atcttcaaac gacatcagcg tagaacttaa
     2761 gccaatgaaa cttccatgaa cacctaaaag taaaataaaa acactcgttt ccaaaaacca
     2821 atatgacaat tgactcaatt gcataaaacc caactcaaag gcattagaga aaaccccaat
     2881 ccacaactct aatttgttct tatataattg aaatcgataa gcttgaaaaa caaattgagc
     2941 cctttaaaca aaggaggttc aaggaattga gccctctaag agttagaaca caaaggagca
     3001 aaatcaaact ggatctatag aaaacgagaa cataaaacaa aaccgaaaaa gaaatccggc
     3061 caaaagctag cctaaaccgc cggagacgga cgacggagaa gctagacaag agccaaccac
     3121 cgttggtaga ggaaccaacc gatggaggac ggcgacaccg gacaattctg ctaaaggagc
     3181 cagaagattc cgggaaagta accgaccaac cacatcacaa gaggcatatg gatgtcgggc
     3241 tgaagcctgt caagcggact ggaccggccc gctagactca cacatcacag tgggtttaat
     3301 aatgttttac tgttaatgaa tgggcctctc aagacaatgt gctaatccat tccttgctag
     3361 tttttgctca acttttagca tgtagtgttt aacgttttcc ttgtgtatgc gtatctaaat
     3421 tggaacttat gtgaccatca agacagtttg gaatttcatc aaagcttgtc ttgtgatgcc
     3481 tcttagtggt atggagcgga tatcaattgt ccaaattgtc ctttggcaga ctcgagaaat
     3541 gttgaaagca aatgaaaccg gtgaaaagga aaaggttcct tttttctcaa gcttttaaaa
     3601 tcaattaaaa ttgtaatggc aacaaaaatt aagtagatta tcttctaaag ttaagaaagt
     3661 catctaaaaa aacaattttg gaattaaatc cctagttaaa cattttgcct ttaactgtag
     3721 tgtttcattt ctgtgcatca aaggttttga aatcatcaaa actgttttgt gaattctcat
     3781 catggacaca tacacataca cacaagagcc aagagttgac ccactcatca acatcatgga
     3841 tctagacggc ttaacgtccc tcttcacact tttttcaaca catgttttat tccctcccaa
     3901 tttctggtac ctattaaaat aaagaatagt aaacaaatca tcaaaactag cacgcaagcc
     3961 acttgtattg gattgacccc tcatgaattc tattctatta attagagata cgtgacgaaa
     4021 cttacaagtg acaacaattt atctaattac taattaacaa ctaattttaa aaatgttatg
     4081 tgattagcaa aatttaaatg tagttacgtt atcgtaattc tggatctatt actataaact
     4141 aaattatttt tgacgtatga ttagataatg taatgataat tgtaaagttt agtttgaaaa
     4201 aaaacaaaat tgaccagaca ctagagtaaa gcttatgagc attgatggaa taaatgatac
     4261 aaagctcttt aacatttgag tattccattc tctcattaac ggattctcta aagtgcgcca
     4321 agcgtgcgca agagccatca acatgagacc gaattctcgc cctatagtga gtcgtattac
     4381 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4441 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     4501 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     4561 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     4621 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     4681 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4741 cagaggtttt caccgtcatc accgaaacgc gcga
//
