LOCUS       sequence-395360-        5630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains HPCA1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP34
FEATURES             Location/Qualifiers
     source          1..5630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             4497..4517
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     complement(5216..5235)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5217..5235)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5245..5267)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5461..5480)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5580..5602
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc taaacataag agaaaacgca agttgatgag
     2281 ggagcagaag ctcaaatgta tgatattttc attgaccaga tgaaggatgg acaaatggat
     2341 gaaatcaggc taaacataag aaagagagct caggacatcc aaatattatc actagaaata
     2401 atttattatg ttgtaaatgt aaatcatgtt taaatattta tcatgcatgt tgaaggttgt
     2461 tttcgggttt tccttatgtg tgttgggttg tttttcttgt ttttatttta attgaatttc
     2521 ctctgtatat agtggctcat tttcttaaca tgaaataaaa aaaatgtgtt catgaaatta
     2581 gaatattttg tgatccttct tacgctgttt agctaatttt ctttatcctt ttggtttgga
     2641 agtgtcaaat ggtgatgcat ttcgagtttg tgagggtata tttgattgcc gtgtttgcca
     2701 aggtacatca taaggaaaac acggctatgg gatcgacctt ccgaatctct ttgccaaggt
     2761 actcaaatac atcaccattt gaccatggtg gtggacgcca tgggatcgac cttccaaatc
     2821 tctttcattt gttcctctta agtcttaact ctattagctt tccattatgg gtatagttca
     2881 tatttgtatg cttttaagtt tctcaaatct ctttcatttg tcagttttta ctttttagga
     2941 ctggtttatt ttgcacccat tccaatcgtg gggttttaag ttttaacccg taaattattt
     3001 ttttgtttat tttgtatttt tgatacgaaa gcccataaat tgtgaagtaa aaaggcccaa
     3061 taggcccata aaattgggag aagagacgat catcggacaa accgagaaag tgaaacaaca
     3121 aagaagaaac ctgactcttc agaaactatt caggcgagag agagagagag aaacaatttt
     3181 ggacaagaac agaaacgatg tacgttttct gtgatctcaa tattagatat agtattccag
     3241 ttaatcggaa acaaagaagc aacaatttgc aaatcacgga ggttgaacca ctggaggagt
     3301 tggattcagt ctggaaggaa atccaagtta accaacaaca cttttagata atcggaacac
     3361 tatattcaat gactattttg attccagaga ctgttttgca acccaaacag atcctgatct
     3421 caagaatagc taccagagta gctatgaagg cagccgtgta tgatgtatca agttcttcac
     3481 atcttcaggt tcaaagctag taaaccattt gagaatccat ccctggcaac attctttcct
     3541 ttctaagaca ttgaatttga tgagctttat acatcttagg taatgttact gttgcaccag
     3601 aactttgtga ttccggcaat accataattt aggagttgta attaatcttc cttgagatat
     3661 tatgcttgta aaacacatgt ttaataagtt attcaagact tttccacaag tcttgttgaa
     3721 cgtttttgaa gttgttgaga gatatcatag aaaattccaa aaataatgaa tcgctagatc
     3781 aatctatatc caaactgatt attaagaccc agtcaataca caaattgcta ttctagcaaa
     3841 tgtaagtggt gaggtgcacc ttaggcttca aatggccacg gcggactgac aaaaccattt
     3901 gcgggattgg atctcttgtt gtttaccatt cataaactgt agtctgtgga ccgaaaaaaa
     3961 aaaaaatcgc cgtagacaaa ctagctactg attttacata ctgtagaaat aaatttggta
     4021 tggagctgac aacatggttt agtccgctcc gcccgtaaca tccattcaaa gccttaacat
     4081 ataacgtagt taacgcgtag agtatttgag tgtatatatg tatcccctaa acacacgtag
     4141 ttgcacgagt gtagcttgtg gacactaaca gtttcagtcc actatcaagt tgatcgacaa
     4201 aacattatgt tgacatacat caatagcttg atcctaccac tacaacattg ctatgatgtt
     4261 tctccacttc ttgttgttgt tgttgtcctt gctacttgta ctctcttagt ctcccctagc
     4321 ttggtaataa agttcatcaa tagccaaatt taaaggtttg gtccttgtaa gcttgacaca
     4381 acacaaatta gttacacatc ttcttctcat agctcacatt tatttatata tgttttgcat
     4441 acccactcga ttattaaata attagttttg aagttctagt caatatttca tcaaatcatc
     4501 atcatcatca tcatcactgt tgttgtaatt agtattagta tatcaagttg gaaaatggct
     4561 tgtcctcatt tttccaacga ggcaacgacc atgactttat tcaatcttgg ttgttaaaaa
     4621 aaacatatca ctgcatagtt gatgaccaca actatattcc acaagtctct ctttctgaga
     4681 tctaacgatt tgggcaaaca aaatcatatt ttattctttg tttttgaagt ttatagatat
     4741 ttttttttgt aatcaataat gagtgtattt aatacagtag ataaataagg gtcctacttt
     4801 agtttcgcat cacgacaact catatgtata ctttaatgta ttgtttcata aattgttcct
     4861 tcccattact caattgacat ttccatttaa caactcttcc caattgtgaa acctttatat
     4921 cccattatca gctgttttaa gttgaccaag ttttttctta tggtcctctt cctattttct
     4981 tcttttgata tacttttttt tttaactcta tttgttcaaa gtcttcgtct tctttttgtt
     5041 aacattattg tttacatgtt tttgaaagag agattataat ttttattaag agattcgtca
     5101 aacctttgtg tagagcacat gcttttttct tagattattt tgagatagga tcagaaaaca
     5161 gaggaagtga taagaggttc tttttgggtt tgaagaacat gagaccgaat tctcgcccta
     5221 tagtgagtcg tattacctgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     5281 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5341 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     5401 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     5461 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     5521 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     5581 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
//
