LOCUS       sequence-395430-        9428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible myc tag truncated SOX9 without DNA-binding domain 
            (noHMG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Yang Y, Gomez N, Infarinato N, Adam RC, Sribour M, Baek I, Laurin M,
            Fuchs E
  TITLE     The pioneer factor SOX9 competes for epigenetic factors to switch 
            stem cell fates.
  JOURNAL   Nat Cell Biol. 2023 Jul 24. doi: 10.1038/s41556-023-01184-y.
  PUBMED    37488435
REFERENCE   2  (bases 1 to 9428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2023 Jul 24. doi: 10.1038/s41556-023-01184-y."
COMMENT     Sequence Label: plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR
FEATURES             Location/Qualifiers
     source          1..9428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(111..130)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      924..1512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1413..1432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1666..1683
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1800..1821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1836..1841,1842..1859,1860..1866)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1874..1890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1879..1901
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1898..1914
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1898..1914
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1933..1953
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1935..1953
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1981..2207
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2208..2388
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2435..2560
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3053..3286
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3471..3515
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3813..3930
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4373..4391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4409..4427
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4445..4463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4481..4499
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4517..4535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4553..4571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4589..4607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4652..4676
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4769..4789
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4790..4819
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        6114..6614
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6533..6551
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6622..7224
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFPATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7121..7141
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7241..7829
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7294..7314)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7712..7723)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7927..8160
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8232..8353
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8269..8288)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8323..8342
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8393..8528
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8548..8567)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8549..8567)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8577..8594)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8577..8593)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8586..8608)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8735..9190
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8822..8841)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9032..9053
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9216..9320
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9321..9389,9390..9428,1..753)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
       61 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      121 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      181 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      241 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      301 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      361 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      421 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
      481 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
      541 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
      601 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
      661 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
      721 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
      781 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
      841 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
      901 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      961 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1021 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1081 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1141 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1201 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1261 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1321 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1381 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1441 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1501 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1561 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1621 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1681 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1741 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     1801 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     1861 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     1921 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     1981 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     2041 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     2101 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     2161 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     2221 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2281 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2341 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2401 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     2461 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     2521 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     2581 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     2641 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     2701 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     2761 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     2821 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     2881 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     2941 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     3001 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     3061 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     3121 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     3181 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     3241 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     3301 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     3361 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     3421 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     3481 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     3541 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     3601 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     3661 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     3721 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     3781 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     3841 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     3901 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     3961 gtttggttag taccgggccc ggtgctttgc tctgagccag cccaccagtt tggaatgact
     4021 cctttttatg acttgaattt tcaagtataa agtctagtgc taaatttaat ttgaacaact
     4081 gtatagtttt tgctggttgg gggaaggaaa aaaaatggtg gcagtgtttt tttcagaatt
     4141 agaagtgaaa tgaaaacttg ttgtgtgtga ggatttctaa tgacatgtgg tggttgcata
     4201 ctgagtgaag ccggtgagca ttctgccatg tcaccccctc gtgctcagta atgtacttta
     4261 cagaaatcct aaactcaaaa gattgatata aaccatgctt cttgtgtata tccggtctct
     4321 tctctgggta gtctcactca gcctgcattt ctgccatcga tggtaccttt actccctatc
     4381 agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg tatgcagact
     4441 ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag tgatagagaa
     4501 cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt actccctatc
     4561 agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg tataagcttt
     4621 aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt cagatcgcct
     4681 ggagcaattc cacaacactt ttgtcttata ccaactttcc gtaccacttc ctaccctcgt
     4741 aaagctagca ccggtcgcca ccatgagccc taagaagaaa cggaaagtgg aacaaaaact
     4801 tatttctgaa gaagatctga atctcctgga ccccttcatg aagatgaccg acgagcagga
     4861 gaagggcctg tctggcgccc ccagccccac catgtcggag gactcggctg gttcgccctg
     4921 tccctcgggc tccggctcgg acacggagaa cacccggccc caggagaaca ccttccccaa
     4981 gggcgagccg gatctgaaga aggagagcga ggaagataag ttccccgtgt gcatccgcga
     5041 ggcggtcagc caggtgctga agggctacga ctggacgctg gtgcccatgc ccgtgcgcgt
     5101 caacggctcc agcaagaaca aggtgaagaa cggacaagcg gaggccgaag aggccacgga
     5161 acagactcac atctctccta atgctatctt caaggcgctg caagccgact ccccacattc
     5221 ctcctccggc atgagtgagg tgcactcccc gggcgagcac tctgggcaat ctcagggtcc
     5281 gccgacccca cccaccactc ccaaaaccga cgtgcaagct ggcaaagttg atctgaagcg
     5341 agaggggcgc cctctggcag aggggggcag acagcccccc atcgacttcc gcgacgtgga
     5401 catcggtgaa ctgagcagcg acgtcatctc caacattgag accttcgacg tcaatgagtt
     5461 tgaccaatac ttgccaccca acggccaccc aggggttccg gccacccacg gccaggtcac
     5521 ctacactggc agttacggca tcagcagcac cgcacccacc cctgcgaccg cgggccacgt
     5581 gtggatgtcg aagcagcagg cgccgccccc tcctccgcag cagcctccgc aggccccgca
     5641 agccccacag gcgcctccgc agcagcaagc acccccgcag cagccgcagg caccccagca
     5701 gcagcaggca cacacgctca ccacgctgag cagcgagcca ggccagtccc agcgaacgca
     5761 catcaagacg gagcagctga gccccagcca ctacagcgag cagcagcagc actccccgca
     5821 acagatctcc tacagcccct tcaaccttcc tcactacagc ccctcctacc cgcccatcac
     5881 ccgctcgcaa tacgactacg ctgaccatca gaactccggc tcctactaca gtcacgcagc
     5941 cggccagggc tcagggctct actccacctt cacttacatg aaccccgcgc agcgccccat
     6001 gtacaccccc atcgctgaca cctccggggt cccttccatc ccgcagaccc acagcccgca
     6061 gcactgggaa caaccagtct acacacagct caccagaccc tgaacgcgtg gggttgcgcc
     6121 ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga
     6181 aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc
     6241 ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc
     6301 gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca
     6361 ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg
     6421 ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg
     6481 tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg
     6541 cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg
     6601 acctctctcc ccgggggatc catggccacc gagtacaagc ccacggtgcg cctcgccacc
     6661 cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6721 acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6781 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     6841 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     6901 tcgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     6961 ctggcgccgc accggcccaa ggagcccgcg tggttcccgg ccaccgtcgg cgtctcgccc
     7021 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     7081 cgcgctgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     7141 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     7201 atgacccgca agcccggtgc ctgaggatcc tctagatcct aatcaacctc tggattacaa
     7261 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7321 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7381 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7441 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7501 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7561 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7621 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7681 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7741 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7801 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gcatcggtac
     7861 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7921 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     7981 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8041 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8101 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8161 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     8221 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8281 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8341 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     8401 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8461 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8521 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     8581 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     8641 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     8701 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     8761 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     8821 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8881 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8941 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     9001 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     9061 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     9121 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     9181 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     9241 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9301 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9361 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9421 ctgaagat
//
