LOCUS       sequence-395619-        5689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains CIPP1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP08
FEATURES             Location/Qualifiers
     source          1..5689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5275..5294)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5276..5294)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5304..5326)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5520..5539)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5639..5661
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tcaaaccaga catacttgca gcttctcact
     2281 ggaattcctg ttcagtagct ttcaaggcgg caaatggcca gatcttccac atctccttaa
     2341 tcaccaagcc catctacatc caactctttc caggatacat gatcaaagca aagtatatag
     2401 gtctgaactc cctggaaaag atttcatact tggagttgat gtccttcata gccttaagaa
     2461 agtatcttgg aatccaaaaa gacttaagca caagaaccaa ctgctaccat ggactaatgt
     2521 ctcccatctc taccccatgg aatatgaaat tctatcaaag acgagattat caaggcttct
     2581 tgtgctactt cacattcaga gttcctcacc aaattttcat cacctttatg aaagaatccg
     2641 gactttttca tatcattacc cttcaagaag aatgaggatg tcatccaaca aaagcaagcc
     2701 ttccaggaat gaatccaaac cactataatc ttgctctcga agaagtcgac caactccaaa
     2761 gagaagacct cattgagaag accacctccc catgggcatg tgaaaccttc tatgttaaca
     2821 aaagagctga acaagtacga ggaaaactga ggttagttat caactaccat ccgctcaacc
     2881 atttccttgc agacaacaag tttcctctac caaagcgaga atttctattc catctaaaaa
     2941 cctctcaaat taattacaaa ctctaacaat cgagaatggg aaaaatgtca aaaaaagcac
     3001 gaactttcaa atttgggaag aaaaaacata aactttcaaa atgccattaa aatcgtaaag
     3061 ttttgtttga cttttgatta aatagtcaag tttcgttgac tttgccattt cgggcatctc
     3121 gttaagtttc cgttaacaaa aaaatcacag aattaacact ccgttaccat tttctgttaa
     3181 aagtgaaacg acgtcgtttt gaaattctaa ataaaagaaa aaagtaaacc cacaaggatt
     3241 cgaacctatg gtcttttgcc tttcaaagaa tcattaaacc actgagccac gagaacatat
     3301 taacaaaatg aaaaacacat atatatataa gatataaata tacaattaat taaaaataac
     3361 caaacaaaga tctatattaa tgcatttaat ttaaattatt ctttcttaaa ttatataaaa
     3421 aaagaaacat atgaaataga gaaaatgaaa tatattgcct aaataaatgg tttcttatat
     3481 tgtttccatc taattatgaa catcacattt aattatttga agatggaatg attatatttt
     3541 atgtttatct tatatatata tatatatata tatattacaa gttaatttat cgaatagata
     3601 tgtttttata attaaaaata tttataaaat taaattgaat aaaattctat gtgcatgtat
     3661 aaattaatcc tatatttatt tgagaaggat tgtataaatt ttagcctatt ttctaatttc
     3721 ataagtttca atttttttca taaaataaaa tgaagaaaaa ataaattttc agataaaaat
     3781 tcaatgcatt tagaagaaat ctctatatgt aaaattaaaa atatgatatt tcgaaatgta
     3841 tatttatggt atgtatattt aatatttgta catatttggt tatttttaat taattgcata
     3901 cgtatatgtt atatagatcc gtttgcaatc tgtactagaa taatatcgtg gccccaatgg
     3961 ttgagtgcat ctgaggaaaa caatacgtat ctggatcgac gtcgttattt tattttagat
     4021 tttcgtggac tcacaatcgt tattttattt tagattttca aacgacgtcg tttcgtctgt
     4081 aacagaacat attaaacgga aaattaacgc tgtatttttt ttgttaacgg aaacttaacg
     4141 acatgtctaa aatggcgaag tcaacgaaac tttactattt tattaaaagt caaacaaaac
     4201 tttacgattt tagtggcatt tttgaagttc atgttttgtt catcccaaat ttgaaagttt
     4261 atgttttttt gacatttttc ccgttctata tttcacaagt tatttttaat tagttttgtg
     4321 tatatatata aatatttcag tttacaataa tatcaaaacg ttatcgtagc atatttggtt
     4381 gagcgcttgt aataattctg gagagtcatg agtttgattc cttcttggca catattgtgt
     4441 aatatatttt cattttgaaa cgacatcgtt tcgcttctaa cggaaaatgt taacgaaaag
     4501 ttaactccgt tgttttgttg ttaacgaaaa cttaacgaca tgtccaaaat agcaaagtca
     4561 acgaaactta attattttat caaaagtcaa acgaaacttt atgattttag tggcatttcg
     4621 aaagttcacg tttttttatc ccaaatttga aagttcatga tttttttgag aatgctaatt
     4681 aatgatttca agagttttct ttaattgaga atgacgtcgt ttagcaaatg aatggataca
     4741 taacatgtct gattactctc ttaaaccagt taacaatatt taaatcatat tgaaaacaag
     4801 aggacaaagc cagagcgact ctgcttttaa gacacggcaa aaaagtcaac caacgaaccc
     4861 aataacaagg aaagagattt gtttttcatt tttaatggaa aaaaaacagc aaaaattgaa
     4921 gtctactcgt cgtagtagtc gtcgtcttcc actaattccg tggttttttt atcgaagact
     4981 tttgaataaa gtacatttgc agagtctgtc aataaccctt tcttcacggt attgctctca
     5041 agccgcgtac tggtaggctc caaaattatc ccttctttat ggtttttgga acctgggttc
     5101 tccgattttg aggtaatcct tatgtgggtt ctttctgatt tcgtgttttg ttttattaaa
     5161 aggacttcta tgtttttttg ggggtttctg aaaagttcgg tttttgcagg ttgttgaaat
     5221 ctttgagtag atttttgtgg aacatctgac tctcaacatg agaccgaatt ctcgccctat
     5281 agtgagtcgt attacctggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5341 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5401 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     5461 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     5521 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     5581 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     5641 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
