LOCUS       sequence-395629-        5010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains DORN1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP36
FEATURES             Location/Qualifiers
     source          1..5010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4596..4615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4597..4615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4625..4647)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4841..4860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4960..4982
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgatgtaaaa tttgaagctt gaagatgaac
     2281 gttgttaaca aaaagttgat atggccgagt tggtccaagg cgccagatta aggttctggt
     2341 ccgaaagggc gtgggttcaa atcccactgt caacattctc tctttttttt tttttaaacg
     2401 tttgcaatat tttactaatg tatatattaa tttatgaaca tacccctttg tgtaattaat
     2461 ttataactat acccttgtta attctctgat ttacaatatt gaaagtcttc tgggaatttt
     2521 gggagacaac atactccaat ttcagtttct tctggaagaa aatcttaatt ttgctacaat
     2581 ggtgaaccat ggaaagaagg aagcctggat tatggctctg gacaaggtga atttcctttc
     2641 tctgcttctg ggtattgatc atcaaacatg agttctggaa tttaaaggtt ttgagcgatt
     2701 ctttagctac gaaatgtgtg aagaaggaat aacttcagtt cagaacaacc aaccaggctc
     2761 tatgtttcaa ttagatacag ctcaaaggtt ttcatctaat ctggatgttc tcatgtggtc
     2821 ttctaaagtc aacaagaagc tctgggttta tggctttggc caagcttcct ttctaatgga
     2881 ttgttttagc tgatctatag ttcggggatg caaatgggtt acggtttctt tctaaggaca
     2941 tgtcttctta ttctgcttat cttactggta attatagcgc tcatggttct tggagtagga
     3001 tatctattca gaaaaaagac aagacatatg tagatgtaat agaggctttc atgcaaaaaa
     3061 gaagtagtag aggcatgaga ggaggttctt cctggtggta aagacacgtg tttctaaatg
     3121 gaattgcttt agctcttagg tagttataca ctgaaatatc aaggcttctc atgttttgta
     3181 aaatgctgag ttaaaggcaa agtgagtagg ttttgtgtga ccatgggtcc aacctagata
     3241 ccgttgtctc tgtgcgaact ttaggtcaaa tcgcatggag cttgcaacaa tggctgtttg
     3301 cacagtaact gacatgtatg cctgtatgct tgagcaaact tttggaaagg aaactgtgta
     3361 agctgggatg ctagctgata gactgtttga aaatgttgga aacagataag atctgagttc
     3421 agcttagaaa atagggtcgc tttgctcaaa tcaaatggtg tgccagaact gaaagcctcc
     3481 atggaagaag tggtgcaata tcttgaagtt ggaacctggt cctattctct gctgattgtt
     3541 ggaatcttcc ttcattagca gttatccaga aaatgttcaa agctaccaca aggagcaggt
     3601 atctgaccat taatctctta tctgatttat cactatacag taatggattt tcaccaagtt
     3661 aaaatttcct gctgccacaa aaaactgcct agctacctct gcggaattta ggttgtctaa
     3721 gcaaactaaa ctttcttcta tctaattttg tcccgctggt ggtgaggcta ctatgacata
     3781 accaaacaat gaaagaatat gctctatgcg attccctctg tctattatct tctctccaat
     3841 cttagtttca gtgatttgaa ttgttgttca tgtgacgatg tattctcctg cacctgtaga
     3901 gctgaattag caagtcctaa agggttaggt tagtaactat atatttttgt caattttcat
     3961 attcaccatt taaagaaaat tcgtagtttt catgaatatg acaggctttc ctaggccttt
     4021 tccctttggt cgggctcata attgtgttga tattattaca aaacttggat tttttattca
     4081 ttctacgtct gaagggtcaa gggcatgttt aaaatatgtt taaaataata tttatttgtc
     4141 tgcgtccttc taaagatggc ttcaaaattc aaatagaaga aaaatccaat gcaagtaggt
     4201 gtgctatcta gtcctacaca cattttaagg aatttataaa tgttagtcat acgcattatt
     4261 attgttccag ggtacctcca tgcaacagtt gcgttgtcta tctaataaat tgagatcata
     4321 taagttaaaa tattgtgtca gaatattttg aaaatcacgt tgaagcttta tggttatctt
     4381 tttaatataa tcgtgttagt ggagtacagt ttgtaaagca aaggtgattc tcttgattaa
     4441 ataaacatct attagaaagt ttgataggct tgaatcactg atacagaagc ttacatcctc
     4501 tttttgtcca ttttttctcc agcttgcgag gttatgattc atcatcagat ccagactctc
     4561 tgattcatca tctgcaacat gagaccgaat tctcgcccta tagtgagtcg tattacctgg
     4621 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     4681 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4741 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     4801 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     4861 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4921 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4981 ggttttcacc gtcatcaccg aaacgcgcga
//
