LOCUS       sequence-395807-       11296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1103
FEATURES             Location/Qualifiers
     source          1..11296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7153..7741
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7206..7226)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7624..7635)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7813..8046
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8124..8245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8161..8180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8215..8234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8285..8420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8440..8459)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8441..8459)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8469..8486)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8469..8485)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8478..8500)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8627..9082
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8714..8733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8924..8945
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9108..9212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9213..9281,9282..10073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9431..9450)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10244..10832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10733..10752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10986..11003
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11120..11141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11156..11161,11162..11179,11180..11186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11194..11210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11199..11221
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11218..11234
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11218..11234
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11253..11273
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11255..11273
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc ttgctgggta agcgctgtga
     4141 cgtccccacc aacggctgcg gacccgaccg ctggaactcc gcgttcaccc gcaaagacga
     4201 gatcatcacc agcctcgtgt ctgccttaga ctccatgtgc tcagcgctgt ccaaactgaa
     4261 cgccgaggtg gcctgtgtcg ccgtgcacga tgagagcgcc tttgtggtgg gcacagagaa
     4321 ggggagaatg ttcctgaatg cccggaagga gctacagtca gacttcctca ggttctgcct
     4381 ctccgcagct cagcacaggg cagcgacatc ccagctcgaa ggccgggtgg tgagacgggt
     4441 gctcactgtg gcctcgcgtg ctctgtgtcc cacaggaggg cccccgtgga aggatccgga
     4501 ggcagagcac cccaagaagg tgcagcgggg cgagggtgga ggccgtagcc tccctcggtc
     4561 ctccctggaa catggctcag atgtgtacct tctgcggaag atggtagagg aggtgtttga
     4621 tgttctttat agcgaggccc tgggaagggc cagtgtggtg ccactgccct atgagaggct
     4681 gctcagggag ccagggctgc tggccgtgca ggggctgccc gaaggcctgg ccttccgaag
     4741 gccagccgag tatgacccca aggccctcat ggccatcctg gaacacagcc accgcatccg
     4801 cttcaagctc aagaggccac ttgaggatgg cgggcgggac tcgaaggccc tggtggagct
     4861 gaacggtgtc tccctgattc ccaaggggtc acgggactgt ggcctgcatg gccaggcccc
     4921 caaggtgcca ccccaggacc tgcccccaac cgccacctcc tcctccatgg ccagcttcct
     4981 gtacagcacg gcgctcccca accacgccat ccgagagctc aagcaggaag caccttcctg
     5041 cccccttgcc cccagcgacc tgggcctgag tcggcccatg ccagagccca aggccaccgg
     5101 tgcccaagac ttctccgact gttgtggaca gaagcccact gggcctggtg ggcctctcat
     5161 ccagaacgtc catgcctcca agcgcattct cttctccatc gtccatgaca agtcagagaa
     5221 gtgggacgcc ttcataaagg aaaccgagga catcaacacg ctccgggagt gtgtgcagat
     5281 cctgtttaac agcagatatg cggaagccct gggcctggac cacatggtcc ccgtgcccta
     5341 ccggaagatt gcctgtgacc cggaggctgt ggagatcgtg ggcatcccgg acaagatccc
     5401 cttcaagcgc ccctgcactt atggagtccc caagctgaag cggatcctgg aggagcgcca
     5461 tagtatccac ttcatcatta agaggatgtt tgatgagcga attttcacag ggaacaagtt
     5521 taccaaagac accacgaagc tggagccagc cagcccgcca gaggacacct ctgcagaggt
     5581 ctctagggcc accgtccttg accttgctgg gaatgctcgg tcagacaagg gcagcatgtc
     5641 tgaagactgt gggccaggaa cctccgggga gctgggcggg ctgaggccga tcaaaattga
     5701 gccagaggat ctggacatca ttcaggtcac cgtcccagac ccctcgccaa cctctgagga
     5761 aatgacagac tcgatgcctg ggcacctgcc atcggaggat tctggttatg ggatggagat
     5821 gctgacagac aaaggtctga gtgaggacgc gcggcccgag gagaggcccg tggaggacag
     5881 ccacggtgac gtgatccggc ccctgcggaa gcaggtggag ctgctcttca acacacgata
     5941 cgccaaggcc attggcatct cggagcccgt caaggtgccg tactccaagt ttctgatgca
     6001 cccggaggag ctgtttgtgg tgggactgcc tgaaggcatc tccctccgca ggcccaactg
     6061 cttcgggatc gccaagctcc ggaagattct ggaggccagc aacagcatcc agtttgtcat
     6121 caagaggccc gagctgctca ctgagggagt caaagagccc atcatggata gtcaagagag
     6181 ggattccggg gaccctctgg tggacgagag cctgaagaga cagggctttc aagaaaatta
     6241 tgacgcgagg ctctcacgga tcgacatcgc caacacacta agggagcagg tccaggacct
     6301 tttcaataag aaatacgggg aagccttggg catcaagtac ccggtccagg tcccctacaa
     6361 gcggatcaag agtaaccccg gctccgtgat catcgagggg ctgcccccag gaatcccgtt
     6421 ccgaaagccc tgtaccttcg gctcccagaa cctggagagg attcttgctg tggctgacaa
     6481 gatcaagttc acagtcacca ggcctttcca aggactcatc ccaaagcctg atgaagatga
     6541 cgccaacaga ctcggggaga aggtgatcct gcgggagcag gtgaaggaac tcttcaacga
     6601 gaaatacggt gaggccctgg gcctgaaccg gccggtgctg gtcccttata aactaatccg
     6661 ggacagccca gacgccgtgg aggtcacggg tctgcctgat gacatcccct tccggaaccc
     6721 caacacgtac gacatccacc ggctggagaa gatcctgaag gcccgagagc atgtccgcat
     6781 ggtcatcatt aaccagctcc aaccctttgc agaaatctgc aatgatgcca aggtgccagc
     6841 caaagacagc agcattccca agcgcaagag aaagcgggtc tcggaaggaa attccgtctc
     6901 ctcttcctcc tcgtcttcct cttcctcgtc ctctaacccg gattcagtgg catcggccaa
     6961 ccagatctca ctcgtgcaat ggccaatgta catggtggac tatgccggcc tgaacgtgca
     7021 gctcccggga cctcttaatt actagtaatg aactagtccg taacttgaaa gtatttcgat
     7081 ttcttggctt tatatatctt gtggaaagga cgaccgccta gtaggcagag ccttaatacg
     7141 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7201 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7261 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7321 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7381 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7441 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7501 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7561 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7621 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7681 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7741 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7801 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     7861 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7921 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7981 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8041 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     8101 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     8161 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8221 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     8281 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8341 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8401 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     8461 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     8521 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     8581 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     8641 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     8701 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8761 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8821 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     8881 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8941 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     9001 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     9061 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     9121 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     9181 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     9241 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     9301 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     9361 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     9421 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     9481 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     9541 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     9601 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9661 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9721 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9781 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9841 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9901 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9961 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    10021 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    10081 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    10141 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    10201 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    10261 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    10321 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    10381 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10441 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10501 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10561 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10621 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10681 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10741 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10801 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10861 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10921 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    10981 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    11041 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    11101 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    11161 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    11221 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    11281 agctggagct gcaagc
//
