LOCUS       sequence-396128-       11083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11083)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1309
FEATURES             Location/Qualifiers
     source          1..11083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6940..7528
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6993..7013)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7411..7422)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7600..7833
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7911..8032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7948..7967)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8002..8021
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8072..8207
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8227..8246)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8228..8246)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8256..8273)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8256..8272)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8265..8287)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8414..8869
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8501..8520)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8711..8732
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8895..8999
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9000..9068,9069..9860)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9218..9237)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10031..10619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10520..10539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10773..10790
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10907..10928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10943..10948,10949..10966,10967..10973)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10981..10997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10986..11008
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11005..11021
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11005..11021
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11040..11060
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11042..11060
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggaa ggagaatcaa gcagatttga
     4141 aatccacact ccagtttctg acaagaaaaa gaaaaagtgt tctatacata aggaaagacc
     4201 tcagaaacat tcccacgaaa ttttcagaga ctcctccctg gtgaatgaac agtctcaaat
     4261 aactaggagg aaaaagagga aaaaagattt ccagcatctc atttcttctc ctttgaaaaa
     4321 atccagaatc tgtgatgaga ctgcaaatgc cacttccaca ctcaaaaaga gaaaaaagag
     4381 aagatatagt gctttggagg tggacgagga agcaggtgtt acagttgtcc ttgtggataa
     4441 agaaaatatt aacaacacac caaagcattt tagaaaggat gttgatgttg tttgtgttga
     4501 tatgagcata gaacagaagt taccaagaaa gcctaaaaca gacaaatttc aggtacttgc
     4561 taagtcacat gcacataaat cagaagccct gcacagtaaa gttagggaga aaaagaataa
     4621 aaagcatcag aggaaagctg catcctggga gagccagcgg gcaagggaca ccctgcctca
     4681 gtcagaatcc caccaggagg agtcctggct ttctgtgggt ccagggggtg aaattacaga
     4741 actaccagca tctgctcata aaaacaagtc taagaaaaaa aagaaaaagt ccagtaaccg
     4801 ggaatatgag acactggcca tgcctgaagg atcgcaagca ggcagagagg ccgggactga
     4861 tatgcaggaa tcccagccta ctgtgggctt ggatgatgaa actccacaac tactaggacc
     4921 tactcacaaa aaaaagtcta agaaaaaaaa gaagaaaaag tccaatcacc aggaatttga
     4981 ggcattggcc atgcctgaag gatcacaagt gggcagtgag gttggggctg atatgcagga
     5041 atcccggcct gctgtgggcc tgcatggtga aactgcagga ataccagcac ctgcttataa
     5101 aaacaagtct aagaaaaaaa agaaaaagtc caatcaccag gaatttgagg cagtggccat
     5161 gcctgagagc ctcgagagtg cataccctga aggatcacag gtgggcagtg aggttgggac
     5221 tgtggaaggc agtacagctc ttaaagggtt caaggaatcc aacagtacaa agaagaagtc
     5281 taagaaaagg aagcttacgt ctgtcaaaag ggcacgagtg tctggtgatg atttttcagt
     5341 gcccagtaag aactctgaga gcacactctt tgattcagta gaaggtgatg gcgccatgat
     5401 ggaagaaggt gtgaaatcta ggccccgaca aaagaaaacc caggcctgtt tggcaagcaa
     5461 gcacgtgcaa gaggcgccaa ggttagaacc tgcaaatgaa gaacacaatg tggaaacagc
     5521 tgaagattcc gaaataagat acttatctgc agattcagga gatgccgatg attcagatgc
     5581 ggatttgggt tctgccgtga aacagcttca ggagttcatt cctaacatca aggacagggc
     5641 caccagcaca atcaagcgga tgtaccggga cgacttggaa cggtttaagg aatttaaagc
     5701 acaaggtgtc gctattaaat ttggcaagtt ttctgtaaag gaaaataagc agttagagaa
     5761 aaatgtggaa gactttctag ccctgacagg cattgagagt gcagacaagc tgctgtacac
     5821 ggacagatat cctgaggaaa aatctgtgat caccaactta aaaaggagat actcgtttag
     5881 attacacatt ggtaggaaca ttgcccggcc ctggaaactt atatactatc gagcaaagaa
     5941 gatgttcgat gtcaacaatt acaaaggcag gtatagcgaa ggagatactg agaagttaaa
     6001 gatgtaccat tctctccttg ggaatgactg gaagacgatt ggtgagatgg tggcccgaag
     6061 tagcctctcc gtggccctca agttctcaca gatcagcagt caaagaaatc gtggtgcttg
     6121 gagtaagtct gaaacccgga aactaatcaa ggctgtcgaa gaagtgattc tgaagaagat
     6181 gtctccccag gagttaaaag aggtggattc caaactccaa gaaaatcctg aaagttgcct
     6241 atcaattgtt cgggaaaaac tctacaaggg catatcttgg gtagaagtag aagctaaagt
     6301 gcaaaccaga aattggatgc agtgtaaaag taagtggaca gaaattctaa ccaagaggat
     6361 gactaatggt cggcgtatct actatggcat gaatgccctg cgggccaagg tcagccttat
     6421 tgaaaggttg tatgaaataa atgtggaaga tactaatgaa atagactggg aagatcttgc
     6481 tagtgccata ggtgatgttc ctccatctta cgttcaaact aaattttcta ggctgaaagc
     6541 tgtctatgtt ccattttggc agaaaaagac ttttccagag atcatcgact acctttatga
     6601 gacgactcta cctttgctga aggaaaagtt agaaaaaatg atggagaaaa aaggcactaa
     6661 aatccagact cctgcagcac ccaagcaagt tttcccattt cgagacatct tttattatga
     6721 agacgatagt gaaggagagg acatagaaaa agaaagcgaa ggccaggcgc catgcatggc
     6781 tcacgcctgt aattccagta ctttgggagg ccaaggccgg tggatcatct agtaatgaac
     6841 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     6901 ggtgatactt acacacatcg ggttacgcgt taagtcgaca atcaacctct ggattacaaa
     6961 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7021 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7081 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7141 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7201 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7261 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7321 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7381 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7441 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7501 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     7561 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7621 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7681 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7741 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7801 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     7861 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     7921 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7981 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8041 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8101 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8161 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     8221 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     8281 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     8341 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     8401 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8461 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8521 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8581 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8641 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8701 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8761 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8821 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     8881 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8941 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9001 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9061 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9121 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9181 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9241 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9301 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9361 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9421 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9481 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9541 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9601 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9661 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9721 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9781 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9841 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9901 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9961 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10021 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10081 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10141 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10201 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10261 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10321 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10381 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10441 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10501 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10561 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10621 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10681 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10741 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10801 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    10861 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    10921 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    10981 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    11041 caattaaccc tcactaaagg gaacaaaagc tggagctgca agc
//
