LOCUS       sequence-396307-       10132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 
            stem loops.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   De Pace R, Ghosh S, Ryan VH, Sohn M, Jarnik M, Rezvan Sangsari P, 
            Morgan NY, Dale RK, Ward ME, Bonifacino JS
  TITLE     Messenger RNA transport on lysosomal vesicles maintains axonal 
            mitochondrial homeostasis and prevents axonal degeneration.
  JOURNAL   Nat Neurosci. 2024 Apr 10. doi: 10.1038/s41593-024-01619-1.
  PUBMED    38600167
REFERENCE   2  (bases 1 to 10132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2024 Apr 10. doi: 10.1038/s41593-024-01619-1."
COMMENT     Sequence Label: PB-Ef1a-SunTag-RPS7-PP7-WPRE
FEATURES             Location/Qualifiers
     source          1..10132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     377..399
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     391..408
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     392..408
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   538..600
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        885..1096
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          1097..2035
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1992..2012
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2051..2060
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2138..2194
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2210..2266
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2282..2338
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2354..2410
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2426..2482
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2498..2554
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2570..2626
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2642..2698
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2714..2770
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2786..2842
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2858..2914
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2930..2986
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3002..3058
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3074..3130
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3146..3202
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3218..3274
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3290..3346
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3362..3418
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3434..3490
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3506..3562
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3578..3634
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3650..3706
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3794..3850
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3896..3985
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     misc_feature    6394..6982
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6447..6467)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6865..6876)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6985..7001)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6986..7002)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     repeat_region   7405..7439
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7930..7946)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7930..7946)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7943..7965)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7954..7970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7978..7984,7985..8002,8003..8008))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8023..8044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8161..8178)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8380..8968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8460..8479)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9139..9930,9931..9999))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9762..9781
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10000..10104)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
      301 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
      361 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcctcg
      421 ttcattcacg tttttgaacc cgtggaggac gggcagactc gcggtgcaaa tgtgttttac
      481 agcgtgatgg agcagatgaa gatgctcgac acgctgcaga acacgcagct agattaaccc
      541 tagaaagata atcatattgt gacgtacgtt aaagataatc atgtgtaaaa ttgacgcatg
      601 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
      661 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
      721 ttattaaaaa aaacaaaaac tcaaaatttc ttctataaag taacaaaact tttatgggcc
      781 cgctctagag aacctgaatg cagtctctcg gagggcgaag aatcctgcag gctcgaggcg
      841 gccgcgtgga taaccgtatt accgccatgc atgtgcccgt cagtgggcag agcgcacatc
      901 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
      961 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     1021 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     1081 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     1141 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
     1201 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
     1261 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
     1321 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
     1381 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
     1441 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
     1501 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
     1561 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
     1621 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     1681 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     1741 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     1801 ccgggcgccg tccaggcacc tcgattagtt ctcgcgcttt tggagtacgt cgtctttagg
     1861 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     1921 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     1981 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     2041 gtgagctagc gccaccatgg atatctggcg acacgattcg aagatcgcgg ccgcaatcat
     2101 cggtccgggt ggatctggag gtggaggttc tggaggagaa gaacttttga gcaagaatta
     2161 tcatcttgag aacgaagtgg ctcgtcttaa gaaaggttct ggcagtggag aagaactgct
     2221 ttcaaagaat taccacctgg aaaatgaggt agctagactg aaaaagggga gcggaagtgg
     2281 ggaggagttg ctgagcaaaa attatcattt ggagaacgaa gtagcacgac taaagaaagg
     2341 gtccggatcg ggtgaggagt tactctcgaa aaattatcat ctcgaaaacg aagtggctcg
     2401 gctaaaaaag ggcagtggtt ctggagaaga gctattatct aaaaactacc acctcgaaaa
     2461 tgaggtggca cgcttaaaaa agggaagtgg cagtggtgaa gagctactat ccaagaatta
     2521 tcatcttgag aacgaggtag cgcgtttgaa gaagggttcc ggctcaggag aggaactgct
     2581 ctcgaagaac tatcatcttg aaaatgaggt cgctcgatta aaaaagggat cgggcagtgg
     2641 tgaggaacta ctttcaaaga attaccacct cgaaaacgaa gtagctcgat taaagaaagg
     2701 ttcagggtcg ggtgaagaat tactgagtaa aaattatcat ctggaaaatg aggtagcgag
     2761 actaaaaaag gggagtggtt ctggcgaaga gttgctatcg aaaaattatc atcttgagaa
     2821 cgaagttgct aggctcaaaa agggctcagg ctcaggcgag gagttgctct cgaaaaacta
     2881 ccacttggaa aatgaggtcg cgaggttgaa aaaggggagc gggtcgggcg aggagttatt
     2941 gagcaaaaac tatcatttag agaacgaagt cgcgcgctta aagaaaggct cgggctcggg
     3001 cgaagaactc ttatcgaaga actaccacct cgaaaatgag gtcgccaggt tgaaaaaggg
     3061 cagtggcagc ggggaggaac tcttgagcaa gaactaccac ttggagaatg aggtcgcgag
     3121 attgaagaaa gggtcgggga gcggcgagga attgctcagc aagaattatc atttggagaa
     3181 cgaagtcgcc aggctcaaga aaggctcggg gtcgggggag gaattgttga gtaaaaacta
     3241 ccacttggaa aatgaagtcg ccaggctcaa aaaagggagt gggagcggcg aagagttatt
     3301 gagcaaaaat taccacttgg agaacgaagt ggcaaggctc aagaaaggga gcggcagcgg
     3361 ggaggagctc ttatcgaaga actaccactt agagaatgaa gtcgcccgct tgaagaaagg
     3421 ctcggggagc ggggaagagc tcttgagcaa gaactaccac ttggaaaatg aggtggcgcg
     3481 cttgaagaaa gggagcggga gcggggaaga gttactatct aagaattatc atctcgagaa
     3541 cgaggtggct cgactaaaga agggctccgg cagtggggag gaactcctgt cgaagaacta
     3601 tcatcttgaa aatgaggttg caagacttaa aaaggggtcc ggatcaggtg aggaactact
     3661 cagtaagaat taccacctgg aaaacgaagt tgcacgtttg aagaaaggat caggatcagg
     3721 cgaagaactg ctctcaaaag attatcattt ggaaaatgag gttgcacgtt taaaaaaggg
     3781 aagtggcagt ggtgaggaac ttctgtcgaa aaattatcat ctcgagaatg aagtagcccg
     3841 acttaaaaag ggttctggct cgggtcggtc cgcgtacgat cgatgcgcgg ccgtttaccc
     3901 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta
     3961 tccatatgac gttccagatt acgctccggc cgccatcgat ccgcgggcag tggaggacag
     4021 cacgctgcaa gtagtggtac gggtgcggcc ccccacccct cgggagctgg acagtcagcg
     4081 gcggccagtg gttcaggtgg tggacgagcg ggtgctggtg tttaaccctg aggagcccga
     4141 tggagggttc cctggcctga aatggggtgg cacccatgat ggccccaaga agaagggcaa
     4201 agacctgacg tttgtctttg accgggtcat gttcagttcg agcgccaaga tcgtgaagcc
     4261 caatggcgag aagccggacg agttcgagtc cggcatctcc caggctcttc tggagctgga
     4321 gatgaactcg gacctcaagg ctcagctcag ggagctgaat attacggcag ctaaggaaat
     4381 tgaagttggt ggtggtcgga aagctatcat aatctttgtt cccgttcctc aactgaaatc
     4441 tttccagaaa atccaagtcc ggctagtacg cgaattggag aaaaagttca gtgggaagca
     4501 tgtcgtcttt atcgctcaga ggagaattct gcctaagcca actcgaaaaa gccgtacaaa
     4561 aaataagcaa aagcgtccca ggagccgtac tctgacagct gtgcacgatg ccatccttga
     4621 ggacttggtc ttcccaagcg aaattgtggg caagagaatc cgcgtcaaac tagatggcag
     4681 ccggctcata aaggttcatt tggacaaagc acagcagaac aatgtggaac acaaggttga
     4741 aactttttct ggtgtctata agaagctcac gggcaaggat gttaattttg aattcccaga
     4801 gtttcaattg taaacaaaaa tgactaaata aaaagtatat attcacagta cgttaacccg
     4861 cggtaataat tcgaagatcg catcgctagc ccggttgtac aggtaccgga tcctaaggta
     4921 cctaattgcc tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc gactctagaa
     4981 accagcagag catatgggct cgctggctgc agtattcccg ggttcattag atcctaaggt
     5041 acctaattgc ctagaaagga gcagacgata tggcgtcgct ccctgcaggt cgactctaga
     5101 aaccagcaga gcatatgggc tcgctggctg cagtattccc gggttcatta gatcctaagg
     5161 tacctaattg cctagaaagg agcagacgat atggcgtcgc tccctgcagg tcgactctag
     5221 aaaccagcag agcatatggg ctcgctggct gcagtattcc cgggttcatt agatcctaag
     5281 gtacctaatt gcctagaaag gagcagacga tatggcgtcg ctccctgcag gtcgactcta
     5341 gaaaccagca gagcatatgg gctcgctggc tgcagtattc ccgggttcat tagatcctaa
     5401 ggtacctaat tgcctagaaa ggagcagacg atatggcgtc gctccctgca ggtcgactct
     5461 agaaaccagc agagcatatg ggctcgctgg ctgcagtatt cccgggttca ttagatccta
     5521 aggtacctaa ttgcctagaa aggagcagac gatatggcgt cgctccctgc aggtcgactc
     5581 tagaaaccag cagagcatat gggctcgctg gctgcagtat tcccgggttc attagatcct
     5641 aaggtaccta attgcctaga aaggagcaga cgatatggcg tcgctccctg caggtcgact
     5701 ctagaaacca gcagagcata tgggctcgct ggctgcagta ttcccgggtt cattagatcc
     5761 taaggtacct aattgcctag aaaggagcag acgatatggc gtcgctccct gcaggtcgac
     5821 tctagaaacc agcagagcat atgggctcgc tggctgcagt attcccgggt tcattagatc
     5881 ctaaggtacc taattgccta gaaaggagca gacgatatgg cgtcgctccc tgcaggtcga
     5941 ctctagaaac cagcagagca tatgggctcg ctggctgcag tattcccggg ttcattagat
     6001 cctaaggtac ctaattgcct agaaaggagc agacgatatg gcgtcgctcc ctgcaggtcg
     6061 actctagaaa ccagcagagc atatgggctc gctggctgca gtattcccgg gttcattaga
     6121 tcctaaggta cctaattgcc tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc
     6181 gactctagaa accagcagag catatgggct cgctggctgc agtattcccg ggttcattag
     6241 atcctaaggt acctaattgc ctagaaagga gcagacgata tggcgtcgct ccctgcaggt
     6301 cgactctaga aaccagcaga gcatatgggc tcgctggctg cagtattccc gggttcatta
     6361 gatctcgcga agggcgaatt caagcttatc gataatcaac ctctggatta caaaatttgt
     6421 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6481 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6541 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6601 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6661 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6721 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6781 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     6841 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6901 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6961 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca
     7021 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     7081 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa ggattctata
     7141 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata acaatataat
     7201 tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca
     7261 cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg
     7321 agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa
     7381 agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt
     7441 taatctagct gcatcaggat catatcgtcg ggtctttttt ccggctcagt catcgcccaa
     7501 gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc gaggttgaag
     7561 cggcatggaa agagtttgcc gaggatgact gctgctgcat tgacgttgag cgaaaacgca
     7621 cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg
     7681 ttcaggccac ctgggatacc agttcgtcgc ggcttttccg gacacagttc cggatggtca
     7741 gcccgaagcg catcagcaac ccgaacaata ccggcgacag ccggaactgc cgtgccggtg
     7801 tgcagattaa tgacagcggt gcggcgctgg gatattacgt cagcgaggac gggtatcctg
     7861 gctggatgcc gcagaaatgg acatggatac cccgtgagtt acccggcggg cgcgcttggc
     7921 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7981 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8041 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8101 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8161 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8221 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8281 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt tggatccggc
     8341 cgcgttgctg gcgttggatc cggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8401 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8461 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8521 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8581 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8641 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8701 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8761 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8821 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8881 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8941 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9001 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9061 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9121 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9181 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9241 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9301 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9361 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9421 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9481 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9541 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9601 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9661 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9721 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9781 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9841 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9901 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9961 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10021 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10081 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
