LOCUS       sequence-396313-        7460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The act-3 promoter drives expression of TurboRFP in the pharynx, 
            spermatheca, and body wall of C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Welker JM, Serobyan V, Zaker Esfahani E, Stainier DYR
  TITLE     Partial sequence identity in a 25-nucleotide long element is 
            sufficient for transcriptional adaptation in the Caenorhabditis 
            elegans act-5/act-3 model.
  JOURNAL   PLoS Genet. 2023 Jun 29;19(6):e1010806. doi: 
            10.1371/journal.pgen.1010806. eCollection 2023 Jun.
  PUBMED    37384903
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2023-06-29"; volume: "19(6):e1010806. doi"; pages: "
            10.1371/journal.pgen.1010806. eCollection 2023 Jun"
COMMENT     Sequence Label: act-3p(long):rfp
FEATURES             Location/Qualifiers
     source          1..7460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4047..4742
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     primer_bind     complement(4817..4834)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4817..4833)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4826..4848)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5042..5061)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5161..5183
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5221..5239)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5307..5411
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5412..5480,5481..6272)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5630..5649)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6443..7031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6932..6951
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7185..7202
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7319..7340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7355..7360,7361..7378,7379..7385)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7393..7409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7398..7420
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7417..7433
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7417..7433
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaatctgcag gaatccacga gacttcttac aactccatca tgaagtgcga cattgatatc
       61 cgtaaggact tgtacgccaa cactgttctt tccggaggaa ccaccatgta cccaggaatt
      121 gctgatcgta tgcagaagga aatcaccgct cttgccccat cgtaagtttt ctatttttgt
      181 tttagttaac aaattaattt gatttttttt cagaaccatg aagatcaaga tcatcgcccc
      241 accagagcgc aagtactccg tctggatcgg aggatctatc ctcgcctccc tctccacctt
      301 ccaacagatg tggatctcca agcaagaata cgacgagtct ggaccatcaa ttgttcatcg
      361 caaatgcttc taaacgtttt aacaatttat gtaatattca cctgaacttt tggaaatgtc
      421 cagataacgt catctgcctt acggattttc acaccccgat tttaaattat aaatctgctt
      481 cgtgtaagca tcgttttaaa atttgtcggt ttattttaat aatatttcta aacgtgaaat
      541 cgtatatata cttttagttt agtggtccaa tgtcatcatt ggtaaacttc atcgtttaac
      601 gtgacctcca agtccagatc gcggttgttg ataagcattt ctggagtcaa cttcttatca
      661 gttcggtgtc gattgcgttc actgttcctg ttgattgaaa tttcccgcgt tacgataatc
      721 tcgtctctcc ctcttcgcca gttgctcttt cgggccgaaa ttttctttat ctaactgttt
      781 ctctataaga tctatctcac attgtcaggt ttttttcttt ctgcgcctcc accagttaga
      841 attgagaacc taatattttg tgtatttatc gtcatttcac tggattttga catggtttct
      901 taaaggtgca cagacttgtt ccgatgggtc ttactgcgca aaaagttcgt ggtactttaa
      961 aaaatgtata ctttgaaaat gtttttaaac aatttttatt attcataata aatccgtgcg
     1021 tctttaaaaa ggtatgtcaa aatctagtga aattgcgatg aaattccaga cttataaaaa
     1081 acgtcgtgaa ttttttgatc tattggcgcg aaacatctag taaatttcca tgactacaca
     1141 tttattgtcc aaagtaaatt ttctgtagaa ttcattgttt attatttgcc gggacctttt
     1201 atttttcgcg tcgactcggt ttcgctatgg atagacattg gtgtgcgcac tccttctgga
     1261 tgttttttcg aacagaacat tttcatgtct ctgttctctt gattttttgc actgtttctc
     1321 acgccatcgt cattgtcgcc gcgtacattt tttccgtccg gccgtccgtc aatggtgact
     1381 ggaggtaccc gccgttactc gataaggcct tttgtggatg gatgactggc ctctcttctt
     1441 ctctcgccat tctcccctct catctttttt ctgtccattg agagggcgca cgcgcctcgc
     1501 ccttcttctc ctctactgga agattggggg acacttcgtg gtgaactggc tgaccatgga
     1561 ataagagaaa cgcgccgcgc atctgcagta tttgaaaaga gcgcccactc cgcgtcatca
     1621 aatcgccact gtcattgggc acagtcgaag ttgtttccga aatcaattca aaactcagac
     1681 tgactctgac tctttgttca tgtactcaat aactttggtg tcatcgatcg ttagtgctat
     1741 ctttctacga tgaaagagta attgtgttga gccgttcccc tacgacaatt tgtcaatttg
     1801 gtttcatgga aaaggaagat cacgtcccta tttctcacac atgagtcacc attccttata
     1861 tattctgtac aaaaacaata tttcttttgt ttttattgcg aaacatcgga aattggaatc
     1921 ggacgttttt gtttcattgc ccgaaacatt gctgagtcat catttctaga aaaaaacaat
     1981 aataatcgtc atttttgaaa atttcagttt cctctcgtct cgaacaaaaa accacttcag
     2041 cattatgtat agtttttgtt cttttcaata aatatacatt cttagccagt ttcgtagttt
     2101 taataattta ttagctgtat agttacttct ccagttaccc aattcaataa cttggttgca
     2161 aaccacttga ttccatgaac ctttctagtt catgaaacgg attgcactaa tatgacataa
     2221 atatacgatt tataaaagtt tcaaactaga aactttatct tcgctaattc ataatatatg
     2281 ttaaggagtt ttaatctaag tcaaaatagt ttaatccaaa tacataggtt ctaaatagta
     2341 ctttcgatat tgatttagga atcatctgta atttctgctc attgaaacct atgtttctct
     2401 atttttttat ttctttcact agaacgttga aaatcattgt cgcttatacc atcattttct
     2461 ctgatcgtca gcatctctct gatttaccat tcgtgtttgg ttctcttgaa atatataatt
     2521 ctcgatgtgc ctctcattgt gccaaaccaa tagctgcggt atatttcttt gccatctctt
     2581 ttctaacgtt ttcatcggca tgctacgcga tttggtgtaa tcttcgcaaa gcaaagctgc
     2641 tgctactgct ctcgatccca ctccatccac cccctcgatt cacagaactg cacaggcgaa
     2701 gtagagtgta aactgtcggg tgctcgaggt gggagaggct ggagatcccg cctcgtactg
     2761 atgggaagat cctcagtcat tcgaagcaaa cgatccaatc gtcttcacat cttgccacag
     2821 ttgccttatt atctcgttca ttgaatttat attaacaggt aatcgtagta ttcatgaata
     2881 atattaatat tagagttcac attaagtttc acagaatttt ggaaaataaa aactgaaaca
     2941 ttaattaacc gttccaaaca ttttcaaata atgaaaattc aaattatttt cgcaaaaatt
     3001 cttgtgcgct ttcttttttt cgtgtctgct tttaaatgcg actgtttgcg atgcactcaa
     3061 ttgattagat actcgttgcc acttgcgaaa atttatttta aaacaaactt aaaaaagcag
     3121 agcactaatc catagttttg ctacgacctc ctcctgtttc ataatttgtt atcgcattgt
     3181 gtgcctgcgc atgtaacact gctcgaattc ttagaaatgc ccgctgaacc gactgtgtgt
     3241 caacgctctc gccccctaaa gcactattga ctttagcgta tttctcagaa tttcaaagaa
     3301 aagactatat ttttagacac ctcttttaga tgttcttttt ttgctaccat gttcatgttt
     3361 gtctccatgt gaatcagtaa tcacagttta ggtttgtcat agagcaagtg ctggtcaggg
     3421 tttagcgcca tcttgcggtc cttcatttca tctatgagtc aacatttgca tatgtacaca
     3481 gaaaaagagc accgcaaaag cgcattttct caaaacttgt gaaaggaccc ggctcgatag
     3541 tcgaacacct tgtttcattt caattttctg tcaacttccg ctgcccatcc tctcgtttga
     3601 gagaaaacgt atcgagaaag caaggtagtg agtcttctga gaattaccac tagtaagctc
     3661 tgccatctcc atctctcgtt ctatcacaga ttttcttcaa aaacaacggg agcaatgtat
     3721 gttgtctctc gccccggtct ctgctcccgc aatctttgta tcccttttgt tctcttagaa
     3781 tgatggcaac cccgcccaca ttttcctctc tcgtttgtct tcagaaagag agacatcctc
     3841 ccgctccctt cccacaccca cttgctcttt tctattgacc acacattatg aagataacca
     3901 tgttactaat caaattcgtg ttcttttcca atttcttttt ctttcataag ctccttttat
     3961 atcttaaatc taaacctttc tcatttttaa aatataactt attttcaggt acattaaaaa
     4021 ctaacccggg ggattttttt tttcagatga gcgagctgat caaggagaac atgcacatga
     4081 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag
     4141 gcaagcccta cgagggcacc cagaccatga agatcaaggt ggtcgagggc ggccctctcc
     4201 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaagcc ttcatcaacc
     4261 acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga
     4321 gaatcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcttccaga
     4381 acggctgcat catctacaac gtcaagatca acggggtgaa cttcccatcc aacggccctg
     4441 tgatgcagaa gaaaacacgc ggctgggagg ccaacaccga gatgctgtac cccgctgacg
     4501 gcggcctgag aggccacagc cagatggccc tgaagctcgt gggcgggggc tacctgcact
     4561 gctccttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggct
     4621 tccacttcgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg
     4681 agcagcacga gatggctgtg gccaagtact gcgacctccc tagcaaactg gggcacagat
     4741 gatccgagct cgattgttat tgttgattgt tacgcgtgat gcaataaaat gtgttatgcg
     4801 ttttaaaccg gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     4861 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     4921 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     4981 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     5041 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     5101 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     5161 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     5221 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     5281 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5341 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5401 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5461 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5521 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5581 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5641 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5701 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5761 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5821 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5881 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5941 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6001 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6061 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6121 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6181 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6241 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6301 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6361 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6421 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6481 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6541 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6601 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6661 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6721 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6781 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6841 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6901 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6961 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7021 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7081 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7141 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7201 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7261 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7321 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7381 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7441 acgccacctt cttgggtatg
//
