LOCUS       sequence-396317-        8699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible lentiviral expression of Cdx2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Xie G, Lee JE, Senft AD, Park YK, Jang Y, Chakraborty S, Thompson 
            JJ, McKernan K, Liu C, Macfarlan TS, Rocha PP, Peng W, Ge K
  TITLE     MLL3/MLL4 methyltransferase activities control early embryonic 
            development and embryonic stem cell differentiation in a 
            lineage-selective manner.
  JOURNAL   Nat Genet. 2023 Apr 3. doi: 10.1038/s41588-023-01356-4.
  PUBMED    37012455
REFERENCE   2  (bases 1 to 8699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2023 Apr 3. doi: 10.1038/s41588-023-01356-4."
COMMENT     Sequence Label: pCW57.1-Cdx2-3xT7
FEATURES             Location/Qualifiers
     source          1..8699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     421..441
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        423..441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        469..695
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             696..876
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    923..1048
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1541..1774
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1959..2003
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2216..2333
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2387..2701
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2395..2413
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2431..2449
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2466..2484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2502..2520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2538..2556
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2573..2591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2609..2627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2674..2698
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3816..4326
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4245..4263
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4336..4932
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4336..4355)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4832..4852
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4933..4986
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4987..5604,5605..5610,5611..5646,5647..5649,5650..5685,
                     5686..5688,5689..5724,5725..5733)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5773..5793)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6191..6202)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6407..6640
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6712..6833
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6749..6768)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6803..6822
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6873..7008
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7008..7079
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7080..7148,7149..7940)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7298..7317)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8111..8699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8600..8619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggttttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      421 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      481 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      541 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      601 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      661 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      721 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      781 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      841 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      901 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      961 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1021 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1081 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1141 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1201 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1261 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1321 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1381 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1441 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1501 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1561 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1621 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1681 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1741 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1801 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1861 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1921 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1981 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     2041 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2101 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2161 caaccccgag gggacaattc tcgacctcga gacaaatggc agtattcatc cacaatttta
     2221 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2281 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2341 attacaggga cagcagagat ccactttggc cgcgaatcga tatgtcgagt ttactcccta
     2401 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag
     2461 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     2521 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc
     2581 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg
     2641 taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2701 ctggagaatt ggaattccgc gaacgcgtgc caccatgtac gtgagctacc ttctggacaa
     2761 ggacgtgagc atgtatccta gctccgtgcg ccactccggc ggcctgaacc tggctccgca
     2821 gaactttgtc agtcctccgc agtacccgga ctacggtggt taccacgtgg cggccgcggc
     2881 ggctgctacg gcgaacttgg acagcgctca gtccccaggg ccatcctggc ccaccgcgta
     2941 cggcgcccct ctccgcgagg actggaatgg ctacgcaccc gggggcgctg cggcagccaa
     3001 cgcggtagcc cacggtctca atggtggctc cccggccgcc gctatgggct acagcagccc
     3061 cgccgaatac cacgcgcacc atcacccgca tcatcacccg caccatccgg ccgcctcgcc
     3121 gtcctgcgcc tccggcttgc tgcagacgct caacctcggc cccccggggc ccgcagccac
     3181 cgccgccgcc gaacagctgt cccccagcgg ccagcggcga aacctgtgcg agtggatgcg
     3241 gaagcccgcg cagcagtccc taggaagcca agtgaaaacc aggacaaaag acaaataccg
     3301 ggtggtgtac acagaccatc agcggctgga gctggagaag gagtttcact ttagtcgata
     3361 catcaccatc aggaggaaaa gtgagctggc tgccacactt gggctctccg agaggcaggt
     3421 taaaatttgg tttcagaacc gcagagccaa ggagaggaaa atcaagaaga agcagcagca
     3481 gcaacagcag cagcagcaac aacagcctcc acagccgccg ccacaacctt cccagcctca
     3541 gccgggtgcc ctgcggagcg tgcccgagcc cttgagtcct gtgacctcct tgcaaggctc
     3601 agtgcctggt tctgtccctg gggttctggg gccagctgga ggggttttaa actccactgt
     3661 cacccaggga tccggtggaa gtatggctag catgactggc gggcagcaaa tgggcgggtc
     3721 tagcgcaatg gcatctatga ccggaggtca acagatggga ggttcctcag ccatggcgag
     3781 tatgacaggt ggccaacaga tgggttgagt taaccggggt tggggttgcg ccttttccaa
     3841 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
     3901 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
     3961 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
     4021 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
     4081 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
     4141 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     4201 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     4261 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     4321 ccccagcaat tcaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4381 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     4441 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     4501 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     4561 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     4621 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     4681 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     4741 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     4801 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     4861 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     4921 aagcccggtg ccgaaggtag aggttctctc ctcacttgtg gtgatgttga agaaaaccct
     4981 ggtccaatgt ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat
     5041 ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag
     5101 cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag
     5161 atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt
     5221 ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa
     5281 gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg
     5341 ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc
     5401 cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga
     5461 gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac
     5521 cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag
     5581 aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac
     5641 atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt
     5701 gacgattttg accttgacat gctccccggg taactaagta aggatcgatc caagatatcg
     5761 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     5821 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     5881 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     5941 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6001 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6061 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     6121 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6181 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6241 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6301 ctccccgcct gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc aatgacttac
     6361 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     6421 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     6481 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6541 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6601 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     6661 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     6721 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6781 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6841 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6901 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6961 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggcctt tcaaatatgt
     7021 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7081 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7141 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7201 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7261 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7321 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7381 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7441 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     7501 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7561 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7621 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7681 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7741 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7801 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7861 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7921 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7981 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8041 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8101 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8161 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8221 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8281 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8341 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8401 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8461 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8521 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8581 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8641 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaa
//
