LOCUS       sequence-396454-        7121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses P1 S tail fibre and U chaperone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Huan YW, Fa-Arun J, Wang B
  TITLE     The Role of O-antigen in P1 Transduction of Shigella flexneri and 
            Escherichia coli with its Alternative S' Tail Fibre.
  JOURNAL   J Mol Biol. 2022 Sep 15;434(21):167829. doi: 
            10.1016/j.jmb.2022.167829.
  PUBMED    36116540
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2022-09-15"; volume: "434(21):167829. doi"; pages: " 
            10.1016/j.jmb.2022.167829"
COMMENT     Sequence Label: 4A3.SU
FEATURES             Location/Qualifiers
     source          1..7121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3364..3381
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3434..3452)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3448..3495
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    3527..3547
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3548..3605
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(3629..3646)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(3908..4858)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4906..5128
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5620..5714)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(5744..6535,6536..6604))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIQYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6367..6386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6605..6709)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6774..6792
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(6796..6839)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    6842..6863
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gttaattacc tcagtggatg gtgataaaac cgcaggcgcg gcgtcggtgg atattggtga
       61 attttaccag gtaactattc agcaacgtaa ggatatttct cttgcactta gtgcaggcaa
      121 gaaatatgca attgtgctga agggaagata cctccttgga gaagatacct accaggtgaa
      181 taccgcgtca catattcatg cggctgaatt tgttgccaga acctataccg attcatatca
      241 gttaggagat ggggagctgc ttgtttgtac ggtgaatatc cctgctagtg tatctgccat
      301 tacccaggag atgattgata catccgagcg tatcaaccgc tcgatcggca ttgatatttc
      361 agactctgta accagtacca gaagtgatgt tgctgcaagt tcgctggcag ttaaaaaagc
      421 ctacgatctg gcgaaaagca agtatacggc acaggatgca agcacaacgc aaaagggatt
      481 agttcagctc agtagcgcaa ctaacagcga cagcgaaaca atggcggcta cccctaaagc
      541 tgttaagtct ataaaagatc tggctgatac caaagcgcca atagaaagcc cgagtctgac
      601 aggaacgcca accgcgccga cggcagcgca aggtacaaac agcacgcaga tcgcaaatac
      661 agcctttgtt aaggcagcta taactgcact tatcaacggt gcgcctggca cactggatac
      721 gctgaaagaa atagcggctg cgatcaataa cgacccgaat tacagcacaa ctatcaacaa
      781 tgccttggct ctcaaagcgc ctttggcaag ccctgcatta acgggtgtcc ctactgcgcc
      841 tacggctgca cagggcacaa acaatacgca gatcgctacg actgcttacg tacgggctgc
      901 tatctctgca ttggtcggct catcacctga agctcttgat accctgtatg agcttgcagc
      961 agcactgggc aatgacccga actttgcgac aacaatgaca aatgcgctgg cagggaaaca
     1021 gccacttgat gcaactttaa ccgcgcttgc tggtcttgcg acaggcgcaa ataaattgcc
     1081 gtactttacc ggtacagaca ctgtttctca gactgactta acgtcagttg gtcgcgatat
     1141 tctggccaaa acaagcattc ttgctgttat ccaatacctt ggtttaagag aactcggtac
     1201 cagcggtgaa aagatccccc tgttgagcac ggctaacaca tggagtgcac gccagacttt
     1261 caacggcggg atcaccgggg cgctgacagg gaacgccgat accgcaacga aattgaaaac
     1321 agccagaaac attaatggcg tcaggttcga tggttctggt gacattaata tcaatactct
     1381 ggtatcgcgc ggtcgcgtaa cggccctgga ggcgaatgca cagggaacat ccgggattca
     1441 gctgtatgag gcatacaaca atggctaccc ttccccctat ggcaatgtgc ttcaccttaa
     1501 aggtgccacc gctgctggcg aaggtgagtt attcattggc tggagtggca cgagcggtgc
     1561 ccatgcgccc gtacatatcc gttcgcggcg ggatactgat tctgccaact ggtctgaatg
     1621 ggcgcaggtc tatacgtcaa aagattcaat tcccggcgtc aatgccaaag gggatcagga
     1681 tacctctggt aatgcggcta cagcgaccaa gttgcagaca gcatgtacta tcaacggcgt
     1741 ctcgtttgac ggttctaaaa atattgagct aacggcggaa gatttaaatc tacaggaaac
     1801 ggtaaacaag gctgataacg cggttcaaaa gacaggcgat accttgtccg gtggacttac
     1861 ttttgaaaac gactcaatcc ttgcctggat tcggaatact gactgggcga agattggttt
     1921 taaaaatgat gccgacagcg atactgattc atacatgtgg tttgaaacag gcgacaacgg
     1981 caatgaatat ttcaaatgga gaagcaaaca aagtaccaca acaaaagacc tgatgaatct
     2041 taaatgggat gctttgtatg ttcttgtcaa tgccattgta aatggcgaag tcatatcaaa
     2101 atcagcaaac ggcctacgta ttgcttatgg taattacgga ttctttattc gtaatgatgg
     2161 ttcaaataca tacttcatgt tgacaaactc cggtgacaac atggggactt ataacggatt
     2221 aaggccatta tggattaata acgctactgg cgctgtttcg atggggcgtg gccttaatgt
     2281 ttcaggggag acactttcag accgttttgc tattaacagc agtaatggta tgtggattca
     2341 gatgcgcgat aacaacgcta tctttgggaa aaatatagtt aacactgata gcgctcaggc
     2401 gttacttcgc cagaatcacg ccgaccgaaa gttcatgata ggtggactgg ggaacaagca
     2461 atttggcatc tacatgatta ataactcaag gacagccaat ggcaccgatg gtcaggcgta
     2521 catggacaat aacggtaact ggctttgcgg tgcgcaaatt attcccggaa attatggcaa
     2581 ttttgactca cgctatgtga gagatgtccg acttggcaca cgtgttgttc aattgatggc
     2641 gcgtggaggg cgttatgaaa tagccggaca cgcacttacc ggattaagga ttattggtga
     2701 agttgatggc gatgatgaag ctatcttcag gccgatacaa aaatacatca atggcatatg
     2761 gtataacgtc gcacaggtgt aaattatgca gcacttaaaa aatatcaggt caggaaaccc
     2821 aaagacaaaa gagcaatacc aattaacaaa gaattttgac gtaatctggt tgtggtctga
     2881 agacggaaaa aactggtatg aggaagtgaa aaactttcaa ccagacacca taaagattgt
     2941 ttacgatgaa aataatatta ttgtcgccat caccaaagat gcctccacgc ttaaccctga
     3001 aggttttagc gtcgttgagg ttcccgacat aacagccaac cgccgcgctg atgattcagg
     3061 aaagtggatg tttaaggatg gagctgtagt taaacggatt tatacggcag acgaacagca
     3121 acaacaagcc gaatcacaaa aggccgcatt gctttccgaa gctgaatcag tcatccagcc
     3181 gctggaacgc gctgtcaggc tgaatatggc aacagacgag gaacgcacac gactggaagc
     3241 atgggaacgc tacagtgttc tggtcagccg tgtggatacg gcaaatcctg aatggccaca
     3301 aaagcctgaa taaaaaagga tccgaattcg agctccgtcg acaagcttgc ggccgcactc
     3361 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
     3421 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     3481 ttgaggggtt ttttgctgaa aggaggaact atatccggat taataatact agtagcggcc
     3541 gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
     3601 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3661 ggctgcggcg agcggtatca gctcactcaa aggcggtaat ctcgaggtta cattgtcgat
     3721 ctgttcatgg tgaacagctt tgaatgcacc aaaaactcgt aaaagctctg atgtatctat
     3781 cttttttaca ccgttttcat ctgtgcatat ggacagtttt ccctttgata tgtaacggtg
     3841 aacagttgtt ctacttttgt ttgttagtct tgatgcttca ctgatagata caagagccat
     3901 aagaacctca gatccttccg tatttagcca gtatgttctc tagtgtggtt cgttgttttt
     3961 gcgtgagcca tgagaacgaa ccattgagat catacttact ttgcatgtca ctcaaaaatt
     4021 ttgcctcaaa actggtgagc tgaatttttg cagttaaagc atcgtgtagt gtttttctta
     4081 gtccgttatg taggtaggaa tctgatgtaa tggttgttgg tattttgtca ccattcattt
     4141 ttatctggtt gttctcaagt tcggttacga gatccatttg tctatctagt tcaacttgga
     4201 aaatcaacgt atcagtcggg cggcctcgct tatcaaccac caatttcata ttgctgtaag
     4261 tgtttaaatc tttacttatt ggtttcaaaa cccattggtt aagcctttta aactcatggt
     4321 agttattttc aagcattaac atgaacttaa attcatcaag gctaatctct atatttgcct
     4381 tgtgagtttt cttttgtgtt agttctttta ataaccactc ataaatcctc atagagtatt
     4441 tgttttcaaa agacttaaca tgttccagat tatattttat gaattttttt aactggaaaa
     4501 gataaggcaa tatctcttca ctaaaaacta attctaattt ttcgcttgag aacttggcat
     4561 agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg attcctgatt tccacagttc
     4621 tcgtcatcag ctctctggtt gctttagcta atacaccata agcattttcc ctactgatgt
     4681 tcatcatctg agcgtattgg ttataagtga acgataccgt ccgttctttc cttgtagggt
     4741 tttcaatcgt ggggttgagt agtgccacac agcataaaat tagcttggtt tcatgctccg
     4801 ttaagtcata gcgactaatc gctagttcat ttgctttgaa aacaactaat tcagacatac
     4861 atctcaattg gtctaggtga ttttaatcac tataccaatt gagatgggct agtcaatgat
     4921 aattacatgt ccttttcctt tgagttgtgg gtatctgtaa attctgctag acctttgctg
     4981 gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg tgtgtttttt
     5041 ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa aagataaaaa
     5101 gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag tattacaaaa
     5161 ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct aaaggcttaa
     5221 gtagcaccct cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg
     5281 cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc tctggcagtg
     5341 aatgggggta aatggcacta caggcgcctt ttatggattc atgcaaggaa actacccata
     5401 atacaagaaa agcccgtcac gggcttctca gggcgtttta tggcgggtct gctatgtggt
     5461 gctatctgac tttttgctgt tcagcagttc ctgccctctg attttccagt ctgaccactt
     5521 cggattatcc cgtgacaggt cattcagact ggctaatgca cccagtaagg cagcggtatc
     5581 atcaacaggc ttacccgtct tactgtccct agtgcttgga ttctcaccaa taaaaaacgc
     5641 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     5701 tatcaacagg agtccaagcg agctcgtaaa cttggtctga cagttaccaa tgcttaatca
     5761 gtgaggcacc tatctcagct atctgtctat ttcgttcatc catagttgcc tgactccccg
     5821 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5881 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5941 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6001 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6061 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6121 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6181 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6241 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6301 caaccaagtc attctgagaa tattgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6361 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6421 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6481 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6541 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6601 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6661 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttccca
     6721 tggtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     6781 gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct
     6841 ggaattcgcg gccgcttcta gagatcaccc cgtccgcagg gcggggtgac aagttactta
     6901 tcttacaatg aggcttcaca acattgatta gggaaaatca tgaatgacgt tacagttgtt
     6961 acatcggtta cttacccatc atccgagtcg ttggctctgg tggccgatgt gcaataccac
     7021 gaaccatatc tgtcagccgc gctaaaccga aaattcaggg ggattgttga cccgggattt
     7081 tatgccggtt tcttacctaa gcctggcggt gggatgaacc t
//
