LOCUS       sequence-396691-       11518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Karsi A, Lawrence ML
  TITLE     Broad host range fluorescence and bioluminescence expression vectors
            for Gram-negative bacteria.
  JOURNAL   Plasmid. 2007 May;57(3):286-95. doi: 10.1016/j.plasmid.2006.11.002
  PUBMED    17207855
REFERENCE   2  (bases 1 to 11518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2006.11"; journalName: "Plasmid"; date: 
            "2007-05"; volume: "57"; issue: "3"; pages: "286-95"
COMMENT     Sequence Label: pAKgfplux1
FEATURES             Location/Qualifiers
     source          1..11518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             417..1079
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     1773..1795
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1787..1804
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1788..1804
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1814..1833
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1814..1832
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1865..1881
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1865..1881
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(1899..3011)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(3190..4173)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             complement(4188..5270)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(5319..6242)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(6255..7697)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             complement(7758..8471)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(7798..7818)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     8413..8441
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(8525..8545)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8525..8543)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8564..8580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8564..8580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8577..8599)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8588..8604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8612..8618,8619..8636,8637..8642))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8657..8678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8906..8977
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8978..9046,9047..9838)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9196..9215)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11163..11518,1..416)
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 gcctgccccc cgagcctcac ggcggcgagt gcgggggttc caagggggca gcgccacctt
       61 gggcaaggcc gaaggccgcg cagtcgatca acaagccccg gaggggccac tttttgccgg
      121 agggggagcc gcgccgaagg cgtgggggaa ccccgcaggg gtgcccttct ttgggcacca
      181 aagaactaga tatagggcga aatgcgaaag acttaaaaat caacaactta aaaaaggggg
      241 gtacgcaaca gctcattgcg gcaccccccg caatagctca ttgcgtaggt taaagaaaat
      301 ctgtaattga ctgccacttt tacgcaacgc ataattgttg tcgcgctgcc gaaaagttgc
      361 agctgattgc gcatggtgcc gcaaccgtgc ggcaccccta ccgcatggag ataagcatgg
      421 ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa gcccggtcac tgggtgcaaa
      481 cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc gaggaaaccc acggcggcaa
      541 tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa cgccgtggtg gtcagccaga
      601 agacactttc caagctcatc ggacgttctt tgcggacggt ccaatacgca gtcaaggact
      661 tggtggccga gcgctggatc tccgtcgtga agctcaacgg ccccggcacc gtgtcggcct
      721 acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga ccagttgcgc ctgtcggtgt
      781 tcagtgccgc cgtggtggtt gatcacgacg accaggacga atcgctgttg gggcatggcg
      841 acctgcgccg catcccgacc ctgtatccgg gcgagcagca actaccgacc ggccccggcg
      901 aggagccgcc cagccagccc ggcattccgg gcatggaacc agacctgcca gccttgaccg
      961 aaacggagga atgggaacgg cgcgggcagc agcgcctgcc gatgcccgat gagccgtgtt
     1021 ttctggacga tggcgagccg ttggagccgc cgacacgggt cacgctgccg cgccggtagc
     1081 acttgggttg cgcagcaacc cgtaagtgcg ctgttccaga ctatcggctg tagccgcctc
     1141 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
     1201 tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccgttttt atcaggctct
     1261 gggaggcaga ataaatgatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa
     1321 actggcctca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg
     1381 taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt
     1441 cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca
     1501 accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc
     1561 atcgcagtac ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     1621 ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga
     1681 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     1741 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     1801 cagtgagcgc gcgtaatacg actcactata gggcgaattg gagctccacc gcggtggcgg
     1861 ccgctctaga actagtggat cccccgggct gcaggtcatc aactatcaaa cgcttcggtt
     1921 aagcttaaag cacacccttt ctgcgtcctc gtattgacgc gacgtaaaat ttcaacgagc
     1981 acgccgggat acttaccata ttctctgcta attatcccga catcatcggt aacaataaat
     2041 gctggataac tggttgctga cgcatccata taactcatca accccggcgt tccatcaggt
     2101 acaggtttca acgtttcagg atcaagcgct cgcgcatata cccacggcgg aacatgttta
     2161 cgctgcattt catcctcaaa gaaacaagtg ttgagttcaa cttgattaaa tatatctcgg
     2221 atctgactaa tatcactgag attgaaagta tcaaataaaa gatgattgaa atcatcacgt
     2281 ttcagagatt ctttttcgta acttttccag ccgcctccgg ttatgatata aaggctttta
     2341 tctccagaaa atgagatttt tttatctttc atataatggc agagtaaata aataaagtat
     2401 ggcgaaccaa taagacaaag atctttccct tgatttttta ttcgttcaag actattcaat
     2461 gttttaacaa aatctattcg ttcttctgtt acggtaaatg tcgtaggata taacaattcc
     2521 accaaactca taacatattt aaaccaaata ttatgagcat taaatctatc tggtcccaaa
     2581 ttgactaatt ctatttgatg atcaaaccaa ctaccaacat atttcatgcc ataactcaca
     2641 gagcctaaga gtctctcaat acttaatctg tcacgcgcca cctgactttt taaaccattc
     2701 gtgccgctac tggtaaacca actttcaatc tcgttttcct gagaagttaa taagcgagta
     2761 aacttaaaaa ccgatgttgg gaatacaggt atgtcatcaa tttccgtaat attgtcatct
     2821 actttgtgtg cctgacagta gtgacgatat tctcgacaat gtttataatg attacgaaat
     2881 gcatcaagca caagtttctt tctgattttt tcctgctcgt cgtaagacca cactaatgga
     2941 tcgctcgaaa aaatcaaatc atcaatttct gagcttgctg taatttcttg tttatcaaca
     3001 tatgaagtca tacctgtttt actcctcaag ataatattag aaagtatggc agcactgctg
     3061 tcatactctt ttataccctt catctttcaa gctgctgctt tgttggctgc tttcactcac
     3121 cccagtcaca tagttatcta tgctcctggg gattcgttca cttgccgcct cgctgcaact
     3181 cgaaatctat taggtatatt ccatgtggta cttcttaata ttatcatcaa caatattgat
     3241 tacatttttt tggctcatca aatcattcat tggttcaaag gacagcaata cacttttcgc
     3301 accacacttt tcaattgcca acttagccgc agttatacac tccgtataat ttccgacagc
     3361 gttttctgca attatttctt caagtttatt ttcgaaattt tcattagggt gcatttcaag
     3421 aacataatca ctaataaatg cacgcgtctc ttgtttagct ttattactat cttcgttata
     3481 gttaactaat atcattaact gatggtctat ctctgatagg tcaacgtcat atttatccgc
     3541 aacggcttta tatctttcag catattcata tctaacatca ttagaatcat cccacttaaa
     3601 gatgagagga ataccttttt tggccgccca ctcaacaata tgatgactgg ttgctgttac
     3661 atatttccga ggtccgcctg gcgtataagc atggggattt acagatattt tagggaagct
     3721 ataaaaatcg ttatctggat tacaatagcc tgttgttaaa gcatcgttaa tgatttcata
     3781 acactcttca aatagttgct gttgatattc aaccgggcga ttaaaaaaat gcatttcatc
     3841 ttttttttcg caatcactaa accctaaaat aaatctccct tcacttaact gatccaataa
     3901 gcaagcttcc tccgctatgc ggacaggatg atgagttgta atgatgtgat ttaatgaacc
     3961 aattttaatt ttctctgtta aaccgagcag aaaaccagaa acagtcagag gagcgccgac
     4021 aacaccatta tctgaaaaat gattttcata cactaaaatc tgttcaaaat tcaacttatc
     4081 aacatactcc gttatttcct gcatgcgaac tatactttgt tcttgaacag ttgttgaatt
     4141 gatgaagtta aggaagaaca atccaaattt catttctttc tccttagcta atataatagc
     4201 gaacgttgtt tttctttaag aaatggcatg acatcagact ggaagagctt catggaagca
     4261 ataatttcgt ctactgttcc attagcttca aatccacaac aaatatttga tattcctgta
     4321 gcatcaatgt ctttttgaat tatgtcaata cattcctgcg gcgttcccac gggattgatt
     4381 tcgtaactgt aatcaatacg gcgattagta tctttatgtc cttttaatac aaagtcacgc
     4441 cactgccctt tattgaaatc ataacctctt gtttggtctg aatcatcaaa aatagtcgta
     4501 gcattcacat aagaatcata ccaatgcccc agaaatttcc ggcaaatctc tttcgcttta
     4561 attgagtcat gatctacaga tgttatatat gataagcaat ggtcgatatt atgaatatcg
     4621 tgcccatatt cttgagccac ttcattataa agctcaagtt gtgctttctt ttcgttagta
     4681 tttataatcc aacttaatat catcggtagg ccaaattgag cagcccactc agtcgtcgaa
     4741 gctgattcag ccaccacata aaccggtgcg ccacctctgc tatacgccgc ggggtttact
     4801 tttaccttat ggaacttgat atgttcatta tcagcttcca tatatccctc tgtcatgcca
     4861 ttctttatca gcccgtacca gcattccgct aaggcgcgac tgttattcat atctgtgccg
     4921 aatacgcgaa agtccttgtt gtaaagccct cggcaaatac caaaccgaaa tcgtcctttt
     4981 gacatttgat ccaataaatt cacatcttca agttggcgta ctggatgggc tgtgggaaga
     5041 acaatagcgg cagttcctac attcaatttt ttagtcgcgc caagtaaata tgcagcagcg
     5101 acataagggt taccaagcaa accaaactcc gtgaaatgat gctccagtaa ccatacggta
     5161 tcaaaaccac actcctcaga gatgcgacct aatttaacca aacgtttcat tacctctgtt
     5221 tgagaaaatt ggggaggttg gtatgtaagc aaaaagtttc caaatttcat agagagtcct
     5281 tatattgcta tttgagtgat agaatatctc aatagatttt aagacagaga aattgcttga
     5341 ttttcaatct caattctcat tcggcgttca ttgactgtcg caatagttaa atgttcaaat
     5401 gacggttcag taatatcaac atcaatatcc agatgatcat tatccatcgc gatagcggct
     5461 ttcgtaaccg attgataaaa attgcgcagg accactaaat tttcactcaa gtcatgcgaa
     5521 cttcctaaca aagaatatat cttgcatcga ttactacgaa tatttgataa caatgtgata
     5581 acttcatctt gcttgaccca attatcgtta tttgcagtaa aagcaataaa cggtatatca
     5641 agatacatca tgttattaat tgtagaagct aaatcttccc aaccaaaatc aagacaatct
     5701 ctcgcaaaga cttcagcacc caatttatgg ccttcaaaat ctagattatt cggcaattca
     5761 ttaatgggta gactgagata atcaaaccct aaagctcttt caagagaata tcttaagtta
     5821 acaacaccga ctgcggtgat taaaaacgaa gcattgattt cagataggct tgcataagct
     5881 atccgcgcag ataagcttga agccaacata ccgaagttat ttatttttcg tgtagttaac
     5941 caatcaacca ctgctaacaa gctctgcttt cctatagaca ttgtaaattc atcaattgtc
     6001 cctgaactca atccaacgtg gtgaagcgaa tcatagcgga tcacatgaaa tccattccgc
     6061 gataaatatt ccgccagacc agcaaaatga tccatcctgc gggcaaaacc agacgcaata
     6121 ataatggcat tctttctctt tgggctgttt tcttctggca gcgtttccca aacatgaatt
     6181 tttttatttc cttcaacaca aataacgtgg tcgatggttt tatattttga ttcattttcc
     6241 atacttttta cctattatgg gacaaataca aggaacttat cttcttccag gaatcgagtc
     6301 tgttctattt caaccgcaac atccttagcc gtatagttag atggcctttc atgagaaata
     6361 tatgtcacta atcgttgcaa cggtctcatt ccgtcatgag atccaccaac tcgaaatatg
     6421 ttattcattc ctgcttctac aatcctttcc gcacctttta atgctaacgc atctcgatat
     6481 ttaaatgatg actcccaagg aaaaatagat atggtttgcg tcttattttt ttgaacataa
     6541 ggcaatattt gctcaatatt atcgacgtga tgaaggtaca cacatctgcc aagtggttga
     6601 ttaaattcca cacctgcatt tgactcaata atcatccaac gttgatgaat atccacctct
     6661 acttttaatc cagcaaacaa gctttctttt tgaactaaag aataggccgc cttttcatca
     6721 aaatcttttt tggcattcgg taatatatgc gcatatagat taagtttttc tatcaacgct
     6781 aacttaaatt cctcataatg atttcccatg taatatatgt tttgggcaga aaaacaagct
     6841 cgctgatcgt aaaaacaaac atcatgagcc gcacctgtcg ctgcggacgt caaatcaaca
     6901 ggattatcga taatgcaaag actcttttta gaaccaaatt taatcacatc agcataagat
     6961 ggcgcatgct ctaccgccca attaatcgca tctggccctc cccaagcgac aataacatcc
     7021 gcatgttgca taatttcttt tgcgagtgat gtatcacctt ggtggggcca atatataaca
     7081 gataaagagc gcgttatcgg atgattaggg tctacatcaa taaaacttaa cgctaatgca
     7141 ttagcggtaa aaggatcggt tgacgatgtt tttataatac actgattctt agttaaaatt
     7201 gcgcgtaata tagacatgat cccagataat ggaacattac ctgccaacag atgtacagat
     7261 ttacctttcg gaaaagcccg aacataactt tcatcctgag gtagccattc atccatgata
     7321 tggcgagaac caagttcatt ttctacaaca tcataaaggc cgcctttaga acataaaatc
     7381 atagatatcc aattggcctc tagcttagcc atttcttctg aatatcccat atattttttt
     7441 aagtcacgaa tgtatgtcct gcgtcttgag tattcttcat ttttccatct ttgccctacc
     7501 gtatagagaa aattgacaat gttatgcaac cgtaattcgt tatttccatt acaatcaata
     7561 atgtttttta catgagagtc attcaatatt ggcaggtaaa cactattatc accaaaatta
     7621 atggattgca ctaaatcatc accttcggga aagatttcaa cctggccgtt aataatgaat
     7681 gaaatttttt tagtcatacg tatcctccaa gcctgaattc gatatcaagc ttgcatgcct
     7741 gcaggtctgg acatttattt gtatagttca tccatgccat gtgtaatccc agcagctgtt
     7801 acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga aagggcagat
     7861 tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt
     7921 tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct aattttgaag
     7981 ttaactttga ttccattctt ttgtttgtct gccatgatgt atacattgtg tgagttatag
     8041 ttgtattcca atttgtgtcc aagaatgttt ccatcttctt taaaatcaat accttttaac
     8101 tcgattctat taacaagggt atcaccttca aacttgactt cagcacgtgt cttgtagttc
     8161 ccgtcatctt tgaaaaatat agttctttcc tgtacataac cttcgggcat ggcactcttg
     8221 aaaaagtcat gctgtttcat atgatctggg tatctcgcaa agcattgaac accataaccg
     8281 aaagtagtga caagtgttgg ccatggaaca ggtagttttc cagtagtgca aataaattta
     8341 agggtaagtt ttccgtatgt tgcatcacct tcaccctctc cactgacaga aaatttgtgc
     8401 ccattaacat caccatctaa ttcaacaaga attgggacaa ctccagtgaa aagttcttct
     8461 cctttactca tatgtatatc tccttcttaa atctagagga tccccgggta cccagctttt
     8521 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     8581 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8641 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8701 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8761 gaggcggttt gcgtattggg cgcatgcata aaaactgttg taattcatta agcattctgc
     8821 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     8881 tgtcgccttg cgtataatat ttgcccattc aaatatgtat ccgctcatga gacaataacc
     8941 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     9001 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     9061 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     9121 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     9181 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     9241 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     9301 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     9361 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     9421 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     9481 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     9541 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     9601 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     9661 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     9721 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     9781 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     9841 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     9901 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccagg tggcactttt
     9961 cggggaaatg tgcgcgcccg cgttcctgct ggcgctgggc ctgtttctgg cgctggactt
    10021 cccgctgttc cgtcagcagc ttttcgccca cggccttgat gatcgcggcg gccttggcct
    10081 gcatatcccg attcaacggc cccagggcgt ccagaacggg cttcaggcgc tcccgaaggt
    10141 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
    10201 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
    10261 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
    10321 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
    10381 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
    10441 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
    10501 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
    10561 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
    10621 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
    10681 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
    10741 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
    10801 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
    10861 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
    10921 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
    10981 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
    11041 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
    11101 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
    11161 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
    11221 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
    11281 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
    11341 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
    11401 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
    11461 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaagggcgaa gcccgccc
//
