LOCUS       sequence-396820-        9686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of human MET receptor tyrosine kinase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-MET-V5/HIS
FEATURES             Location/Qualifiers
     source          1..9686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..59)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        231..610
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        611..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     765..785
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     859..878
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        859..877
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5180..5221
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5231..5248
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5271..5288)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5277..5501
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5547..5975
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5634..5653)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5844..5865
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5984..6004)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5989..6318
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6169..6304
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6231..6250
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6385..7179
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6439..6458)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7049..7068
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7355..7476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7392..7411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7446..7465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7525..7541)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7525..7541)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7538..7560)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7549..7565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7573..7579,7580..7597,7598..7603))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7618..7639
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7756..7773)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7927..8515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8007..8026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8686..9477,9478..9546))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9309..9328
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9547..9651)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
       61 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
      121 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
      181 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      241 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      301 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      361 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      421 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      481 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      541 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      601 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      661 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      721 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      781 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
      841 ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg gctagttaag
      901 cttggtaccg agctcggatc cagtaccctt caccatgaag gcccccgctg tgcttgcacc
      961 tggcatcctc gtgctcctgt ttaccttggt gcagaggagc aatggggagt gtaaagaggc
     1021 actagcaaag tccgagatga atgtgaatat gaagtatcag cttcccaact tcaccgcgga
     1081 aacacccatc cagaatgtca ttctacatga gcatcacatt ttccttggtg ccactaacta
     1141 catttatgtt ttaaatgagg aagaccttca gaaggttgct gagtacaaga ctgggcctgt
     1201 gctggaacac ccagattgtt tcccatgtca ggactgcagc agcaaagcca atttatcagg
     1261 aggtgtttgg aaagataaca tcaacatggc tctagttgtc gacacctact atgatgatca
     1321 actcattagc tgtggcagcg tcaacagagg gacctgccag cgacatgtct ttccccacaa
     1381 tcatactgct gacatacagt cggaggttca ctgcatattc tccccacaga tagaagagcc
     1441 cagccagtgt cctgactgtg tggtgagcgc cctgggagcc aaagtccttt catctgtaaa
     1501 ggaccggttc atcaacttct ttgtaggcaa taccataaat tcttcttatt tcccagatca
     1561 tccattgcat tcgatatcag tgagaaggct aaaggaaacg aaagatggtt ttatgttttt
     1621 gacggaccag tcctacattg atgttttacc tgagttcaga gattcttacc ccattaagta
     1681 tgtccatgcc tttgaaagca acaattttat ttacttcttg acggtccaaa gggaaactct
     1741 agatgctcag acttttcaca caagaataat caggttctgt tccataaact ctggattgca
     1801 ttcctacatg gaaatgcctc tggagtgtat tctcacagaa aagagaaaaa agagatccac
     1861 aaagaaggaa gtgtttaata tacttcaggc tgcgtatgtc agcaagcctg gggcccagct
     1921 tgctagacaa ataggagcca gcctgaatga tgacattctt ttcggggtgt tcgcacaaag
     1981 caagccagat tcggccgaac caatggatcg atctgccatg tgtgcattcc ctatcaaata
     2041 tgtcaacgac ttcttcaaca agatcgtcaa caaaaacaat gtgagatgtc tccagcattt
     2101 ttacggaccc aatcatgagc actgctttaa taggacactt ctgagaaatt catcaggctg
     2161 tgaagcgcgc cgtgatgaat atcgaacaga gtttaccaca gctttgcagc gcgttgactt
     2221 attcatgggt caattcagcg aagtcctctt aacatctata tccaccttca ttaaaggaga
     2281 cctcaccata gctaatcttg ggacatcaga gggtcgcttc atgcaggttg tggtttctcg
     2341 atcaggacca tcaacccctc atgtgaattt tctcctggac tcccatccag tgtctccaga
     2401 agtgattgtg gagcatacat taaaccaaaa tggctacaca ctggttatca ctgggaagaa
     2461 gatcacgaag atcccattga atggcttggg ctgcagacat ttccagtcct gcagtcaatg
     2521 cctctctgcc ccaccctttg ttcagtgtgg ctggtgccac gacaaatgtg tgcgatcgga
     2581 ggaatgcctg agcgggacat ggactcaaca gatctgtctg cctgcaatct acaaggtttt
     2641 cccaaatagt gcaccccttg aaggagggac aaggctgacc atatgtggct gggactttgg
     2701 atttcggagg aataataaat ttgatttaaa gaaaactaga gttctccttg gaaatgagag
     2761 ctgcaccttg actttaagtg agagcacgat gaatacattg aaatgcacag ttggtcctgc
     2821 catgaataag catttcaata tgtccataat tatttcaaat ggccacggga caacacaata
     2881 cagtacattc tcctatgtgg atcctgtaat aacaagtatt tcgccgaaat acggtcctat
     2941 ggctggtggc actttactta ctttaactgg aaattaccta aacagtggga attctagaca
     3001 catttcaatt ggtggaaaaa catgtacttt aaaaagtgtg tcaaacagta ttcttgaatg
     3061 ttatacccca gcccaaacca tttcaactga gtttgctgtt aaattgaaaa ttgacttagc
     3121 caaccgagag acaagcatct tcagttaccg tgaagatccc attgtctatg aaattcatcc
     3181 aaccaaatct tttattagtg gtgggagcac aataacaggt gttgggaaaa acctgaattc
     3241 agttagtgtc ccgagaatgg tcataaatgt gcatgaagca ggaaggaact ttacagtggc
     3301 atgtcaacat cgctctaatt cagagataat ctgttgtacc actccttccc tgcaacagct
     3361 gaatctgcaa ctccccctga aaaccaaagc ctttttcatg ttagatggga tcctttccaa
     3421 atactttgat ctcatttatg tacataatcc tgtgtttaag ccttttgaaa agccagtgat
     3481 gatctcaatg ggcaatgaaa atgtactgga aattaaggga aatgatattg accctgaagc
     3541 agttaaaggt gaagtgttaa aagttggaaa taagagctgt gagaatatac acttacattc
     3601 tgaagccgtt ttatgcacgg tccccaatga cctgctgaaa ttgaacagcg agctaaatat
     3661 agagtggaag caagcaattt cttcaaccgt ccttggaaaa gtaatagttc aaccagatca
     3721 gaatttcaca ggattgattg ctggtgttgt ctcaatatca acagcactgt tattactact
     3781 tgggtttttc ctgtggctga aaaagagaaa gcaaattaaa gatctgggca gtgaattagt
     3841 tcgctacgat gcaagagtac acactcctca tttggatagg cttgtaagtg cccgaagtgt
     3901 aagcccaact acagaaatgg tttcaaatga atctgtagac taccgagcta cttttccaga
     3961 agatcagttt cctaattcat ctcagaacgg ttcatgccga caagtgcagt atcctctgac
     4021 agacatgtcc cccatcctaa ctagtgggga ctctgatata tccagtccat tactgcaaaa
     4081 tactgtccac attgacctca gtgctctaaa tccagagctg gtccaggcag tgcagcatgt
     4141 agtgattggg cccagtagcc tgattgtgca tttcaatgaa gtcataggaa gagggcattt
     4201 tggttgtgta tatcatggga ctttgttgga caatgatggc aagaaaattc actgtgctgt
     4261 gaaatccttg aacagaatca ctgacatagg agaagtttcc caatttctga ccgagggaat
     4321 catcatgaaa gattttagtc atcccaatgt cctctcgctc ctgggaatct gcctgcgaag
     4381 tgaagggtct ccgctggtgg tcctaccata catgaaacat ggagatcttc gaaatttcat
     4441 tcgaaatgag actcataatc caactgtaaa agatcttatt ggctttggtc ttcaagtagc
     4501 caaaggcatg aaatatcttg caagcaaaaa gtttgtccac agagacttgg ctgcaagaaa
     4561 ctgtatgctg gatgaaaaat tcacagtcaa ggttgctgat tttggtcttg ccagagacat
     4621 gtatgataaa gaatactata gtgtacacaa caaaacaggt gcaaagctgc cagtgaagtg
     4681 gatggctttg gaaagtctgc aaactcaaaa gtttaccacc aagtcagatg tgtggtcctt
     4741 tggcgtgctc ctctgggagc tgatgacaag aggagcccca ccttatcctg acgtaaacac
     4801 ctttgatata actgtttact tgttgcaagg gagaagactc ctacaacccg aatactgccc
     4861 agacccctta tatgaagtaa tgctaaaatg ctggcaccct aaagccgaaa tgcgcccatc
     4921 cttttctgaa ctggtgtccc ggatatcagc gatcttctct actttcattg gggagcacta
     4981 tgtccatgtg aacgctactt atgtgaacgt aaaatgtgtc gctccgtatc cttctctgtt
     5041 gtcatcagaa gataacgctg atgatgaggt ggacacacga ccagcctcct tctgggagac
     5101 atcagcaaag ggtcaagaca attctgcaga tatccagcac agtggcggcc gctcgagtct
     5161 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     5221 gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
     5281 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5341 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5401 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5461 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     5521 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     5581 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5641 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5701 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5761 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5821 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5881 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5941 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     6001 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6061 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6121 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     6181 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6241 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6301 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     6361 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     6421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     6601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6661 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6901 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     7021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     7081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     7141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgcga
     7201 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     7261 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7321 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7381 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7441 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7501 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7561 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7621 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7681 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7741 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7801 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7861 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7921 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7981 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8041 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8101 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8161 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8221 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8281 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8341 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8401 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8461 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8521 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8581 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8641 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8701 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8761 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8821 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8881 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8941 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9001 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9061 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9121 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9181 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9241 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9301 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9361 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9421 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9481 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9541 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9601 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9661 ccgaaaagtg ccacctgacg tcgacg
//
