LOCUS       sequence-396872-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for DD neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH52 - [DDp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1909..1925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1909..1925)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1922..1944)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2097..2685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2177..2196)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2802..2826)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2926..3717,3718..3786))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3549..3568
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3787..3891)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3990..4012
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4004..4021
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4005..4021
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4645..4663,1..15))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 attatacgaa gttatgatat caaaaatgat tacgccaagc tgtaagttta aacatgatct
       61 tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca
      121 acgatggata cgctaatgcc ttctccagaa tgtttttggt ttctgatcga taaatgttta
      181 ctggttgttg atgtttgtaa aagtcctgca aattctagaa aattctcaaa ctctttttct
      241 tattttcatg agaaaaccga aactttgaag tacataagca aagagttgcg ttgttatttt
      301 aatcttttga acctattaat aaatcttttt tgttttctcg ggattatgtt aggattacaa
      361 agacatttaa gacaagaagt tgtttctgtt atttcacttt ttttgtttgc gcattcacaa
      421 ttttgttaaa acaaaaaaaa agagaccaaa aatggggccc tctaagggcg aagaattgtt
      481 cacgggagta gtcccaatct tagtcgagct tgacggaggt gagttttatt atcgattttc
      541 gggattttct acttgtaatt accttttgtt tttaataagt tgcttttttc cagatgttaa
      601 tggccacaag ttttccgtgt ctggagaagg agaaggcgac gctacctacg gtaagctgac
      661 cctgaagttc atttgcacta ctggcaagtt accagtacca tggccgaccc tggtgacaac
      721 attttgctac ggggttcaat gcttctccag atacccaggt aggttttcag tttcaatttt
      781 tgttgcaact acctcaactc tatgttttca gatcatatga aaagacacga cttttttaaa
      841 tccgccatgc cagagggtta cgttcaagaa cgtaccattt tttttaagga cgatggtaac
      901 tacaagacac gtgcggaggt taaatttgaa ggcgatactc tcgtgaaccg tattgagctc
      961 aaggtaaatt tttcaagctt ctaaattcca aaataaacca ttttaatttc ttaattccag
     1021 ggaatcgatt tcaaggaaga tggaaacatt ttgggccaca agcttgaata taattataat
     1081 agtcataatg tctacattat ggccgacaaa caaaaaaacg gtattaaggt taacttcaag
     1141 attcgccaca atatcgaaga tggatctgtc caacttgctg accactacca acaaaatact
     1201 ccaattggag atggaccggt tttgcttcca gataaccact acctttccac tcagtctgcc
     1261 ctctctaagg atccgaatga aaaacgtgac catatggttc ttttggagtt cgttaccgca
     1321 gcaggaatta ctcacggaat ggatgaactg tacaagcccg ggtgatggtc tctgagctat
     1381 gcaaaatcct ttcaagcatt cccttcttct ctatcactct tctttctttt tgtcaaaaaa
     1441 ttctctcgct aatttatttg cttttttaat gttattattt tatgactttt tatagtcact
     1501 gaaaagtttg catctgagtg aagtgaatgc tatcaaaatg tgattctgat atccaggaaa
     1561 cggagaatct gtgcggcaat gtcttatcat ttgaaacgcg ccgtatcgat gctgacatca
     1621 tgcggctccc gtgttagtcg agaagatggg tacgggcctt tattcatcca acactctacg
     1681 gcttcttcaa gagctagtag ggcaccctgt agttggaaag ggattatttc gtatggcgag
     1741 cccataccgt ctttcttgcg gaagcttaac acgataggaa gttggatagt ttcgaacgat
     1801 ggttccatac ggaacgctgt ctcaaggatg agtctgacgg agtgtaactc gaaggctagg
     1861 tggaggctca gtgatgataa gtctgcgatg gtggatgcat gtgtcatggt catagctgtt
     1921 tcctgtgtga aattgttatc cgctcagagg gcacaatcct attccgcgct atccgacaat
     1981 ctccaagaca ttaggtggag ttcagttcgg cgtatggcat atgtcgctgg aaagaacatg
     2041 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2101 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2161 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2221 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2281 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2341 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2401 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2461 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2521 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2581 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2641 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2701 ttttctacgg ggtctgacgc tctattcaac aaagccgccg tcccgtcaag tcagcgtaaa
     2761 tgggtagggg gcttcaaatc gtcctcgtga taccaattcg gagcctgctt ttttgtacaa
     2821 acttgttgat aatggcaatt caaggatctt cacctagatc cttttaaatt aaaaatgaag
     2881 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2941 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3001 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3061 accgcgagag ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3121 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3181 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3241 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3301 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3361 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3421 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3481 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3541 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3601 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3661 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3721 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3781 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3841 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3901 ccgaaaagtg ccagatacct gaaacaaaac ccatcgtacg gccaaggaag tctccaataa
     3961 ctgtgatcca ccacaagcgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     4021 tcatgcataa tccgcacgca tctggaataa ggaagtgcca ttccgcctga cctaagtgct
     4081 tttcgagatc gccaaaccgg agaacgatca ggagaatgat gagtcgccac aggagtcggc
     4141 aagatacgtg cgttatagat ttgctccaaa ctttttgaaa ctcaaaacgg tcggagcaac
     4201 tgtggaattc aaagtaggag acatcccaat ccatcatttc cgaatgatcg aacgtcactt
     4261 cttcaaagat cgccttctga agtgttttga ctttgaattc ggattctgta ttccgaattc
     4321 acgaaacaac tgtgaacata tctatgagtt ccctcaactc tctcaacaac tcagtgagtc
     4381 attattctaa aaagtacaat tcaaaagact aatctctttt tcagtggacg acatgatcaa
     4441 caatccaaac gagactcgtt ctgacagctt ctatttcgtc gataacaaac tcgtcatgca
     4501 caacaaagcc gactactcat atgatgcata aatcttcatt gttgagttta tcttgttgat
     4561 ttttgaataa attatcaact ctttactttt taatgggtta tgaaataaat aaacattgaa
     4621 aactgataaa caacgttcat ctctataact tcgtatagca tac
//
