LOCUS       sequence-396873-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for AVK neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH53 - [AVKp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1891..1907)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1891..1907)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1904..1926)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2079..2667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2159..2178)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2784..2808)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2908..3699,3700..3768))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3531..3550
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3769..3873)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3972..3994
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3986..4003
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3987..4003
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4627..4645,1..15))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 attatacgaa gttatgatat caaaaatgat tacgccaagc tgtaagttta aacatgatct
       61 tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca
      121 acgatggata cgctaatgcc gttttgtaat ttaaatatta ggttgaattg tttgacttta
      181 aactaaaaaa gctttactgc gacttccgat tgttttaatt gaaataaaag aattattcat
      241 atctgtgaga gtgaagaata gaaaagaaca ggtggaaatc aaaatatcga ggatacaaaa
      301 tcagctgcca aaaagcccga gaggccttgt gattttgcca caaaatactt tataacttta
      361 gtctcagttt gttgtcggtg cctttttcct aacttccatc aagtgttttt atatcaacta
      421 aaagccgaaa tgaactaaaa agagaccaaa aatggggccc gtttcaaagg gagaggccgt
      481 tataaaagaa tttatgcgtt tcaaggtcca catggaggga tccatgaacg gacacgagtt
      541 cgagatcgag ggagagggag agggacgtcc atacgaggga acccaaaccg ccaaggtgag
      601 ttttattatc gattttcggg attttctact tgtaattacc ttttgttttt aataagttgc
      661 ttttttccag ctcaaggtca ccaagggagg accactccca ttctcctggg acatcctctc
      721 cccacaattc atgtacggat cccgtgcctt caccaagcac ccagccgaca tcccagacta
      781 ctacaagcaa tccttcccag agggattcaa gtgggagcgt gtcatgaact tcgaggacgg
      841 aggagccgtc accgtcaccc aaggtaggtt ttcagtttca atttttgttg caactacctc
      901 aactctatgt tttcagacac ctccctcgag gacggaaccc tcatctacaa ggtcaagctc
      961 cgtggaacca acttcccacc agacggacca gtcatgcaaa agaagaccat gggatgggag
     1021 gcctccaccg agcgactcta cccagaggac ggagtcctca agggagacat caaggtaaat
     1081 ttttcaagct tctaaattcc aaaataaacc attttaattt cttaattcca gatggccctc
     1141 cggctcaagg acggaggacg ttacctcgcc gacttcaaga ccacctacaa ggccaagaag
     1201 ccagtccaaa tgccaggagc ctacaacgtc gaccgtaagc tcgacatcac ctcccacaac
     1261 gaggactaca ccgtcgtcga gcaatacgag cgttccgagg gacgtcactc caccggagga
     1321 atggacgagc tctacaagcc cgggtgatgg tctctgagct atgcaaaatc ctttcaagca
     1381 ttcccttctt ctctatcact cttctttctt tttgtcaaaa aattctctcg ctaatttatt
     1441 tgctttttta atgttattat tttatgactt tttatagtca ctgaaaagtt tgcatctgag
     1501 tgaagtgaat gctatcaaaa tgtgattctg atatccagga aacggagaat ctgtgcggca
     1561 atgtcttatc atttgaaacg cgccgtatcg atgctgacat catgcggctc ccgtgttagt
     1621 cgagaagatg ggtacgggcc tttattcatc caacactcta cggcttcttc aagagctagt
     1681 agggcaccct gtagttggaa agggattatt tcgtatggcg agcccatacc gtctttcttg
     1741 cggaagctta acacgatagg aagttggata gtttcgaacg atggttccat acggaacgct
     1801 gtctcaagga tgagtctgac ggagtgtaac tcgaaggcta ggtggaggct cagtgatgat
     1861 aagtctgcga tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt gaaattgtta
     1921 tccgctcaga gggcacaatc ctattccgcg ctatccgaca atctccaaga cattaggtgg
     1981 agttcagttc ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa aggccagcaa
     2041 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2101 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2161 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2221 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2281 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2341 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2401 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2461 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2521 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2581 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2641 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2701 gctctattca acaaagccgc cgtcccgtca agtcagcgta aatgggtagg gggcttcaaa
     2761 tcgtcctcgt gataccaatt cggagcctgc ttttttgtac aaacttgttg ataatggcaa
     2821 ttcaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2881 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2941 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3001 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag agccacgctc
     3061 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3121 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3181 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3241 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3301 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3361 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3421 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3481 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3541 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3601 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3661 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3721 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3781 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3841 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccagatac
     3901 ctgaaacaaa acccatcgta cggccaagga agtctccaat aactgtgatc caccacaagc
     3961 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtcatgcat aatccgcacg
     4021 catctggaat aaggaagtgc cattccgcct gacctaagtg cttttcgaga tcgccaaacc
     4081 ggagaacgat caggagaatg atgagtcgcc acaggagtcg gcaagatacg tgcgttatag
     4141 atttgctcca aactttttga aactcaaaac ggtcggagca actgtggaat tcaaagtagg
     4201 agacatccca atccatcatt tccgaatgat cgaacgtcac ttcttcaaag atcgccttct
     4261 gaagtgtttt gactttgaat tcggattctg tattccgaat tcacgaaaca actgtgaaca
     4321 tatctatgag ttccctcaac tctctcaaca actcagtgag tcattattct aaaaagtaca
     4381 attcaaaaga ctaatctctt tttcagtgga cgacatgatc aacaatccaa acgagactcg
     4441 ttctgacagc ttctatttcg tcgataacaa actcgtcatg cacaacaaag ccgactactc
     4501 atatgatgca taaatcttca ttgttgagtt tatcttgttg atttttgaat aaattatcaa
     4561 ctctttactt tttaatgggt tatgaaataa ataaacattg aaaactgata aacaacgttc
     4621 atctctataa cttcgtatag catac
//
