LOCUS       sequence-397237-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0198
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6319..6907
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6372..6392)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6790..6801)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6979..7212
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7290..7411
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7327..7346)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7381..7400
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7451..7586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7606..7625)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7607..7625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7635..7652)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7635..7651)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7644..7666)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7793..8248
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7880..7899)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8090..8111
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8274..8378
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8379..8447,8448..9239)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8597..8616)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9410..9998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9899..9918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10152..10169
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10286..10307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10322..10327,10328..10345,10346..10352)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10360..10376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10365..10387
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10384..10400
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10384..10400
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10419..10439
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10421..10439
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcag aattccgagg gtggagcgga
     4141 ttcgccagcg tccgtggctc tgcgtccctc ggcggcagcc ccgcctgtgc cagcctcccc
     4201 gcagagggtg ttggtccagg cagccagctc caatcccaaa ggggcccaga tgcagccgat
     4261 ctccctcccc agagttcagc aggtacccca gcaggtgcag ccggtgcagc acgtgtatcc
     4321 tgcccaggtg cagtacgtgg aagggggaga cgccgtctac accaatggag ccatacgaac
     4381 agcctacacc tacaaccccg agcctcagat gtacgccccc agcagcacgg cttcttactt
     4441 cgaggcccca ggcggtgccc aggtgaccgt ggcagcctcg tccccgccag cggtcccctc
     4501 ccacagcatg gtgggcatca ccatggatgt cggggggagc cccatcgtct ccagcgcggg
     4561 agcctatctc atccacgggg ggatggacag caccagacac tccctggccc acacctcccg
     4621 ctcatcgccc gccacgctcc agtggctgtt ggataattat gaaacagcgg aaggtgtgag
     4681 tctccccaga agttctcttt acaaccacta ccttcggcac tgccaggagc acaagctaga
     4741 cccagtgaac gccgcctcct tcgggaaact gatccgttct gtgtttatgg ggctgagaac
     4801 gcggcggctg ggcaccaggg gcaactcgaa gtaccattac tatgggattc gtctgaagcc
     4861 ggactcacca ctgaaccggc tgcaggagga cacgcagtac atggccatgc ggcagcagcc
     4921 catgcaccag aagcccaggt accggccagc ccagaagacg gacagcctcg gggacagcgg
     4981 ctcccacagc ggcctgcaca gcactccgga acagaccatg gccgtgcaga gccagcacca
     5041 ccagcagtac atagatgtct cccacgtctt ccccgagttc ccagcgcccg acctgggcag
     5101 cttcctgctg caggacggcg tcacactgca cgacgtcaag gccctgcagc tggtgtacag
     5161 acggcactgc gaggcaactg tagatgtggt gatgaacctc cagttccact acatcgagaa
     5221 gctgtggctc tccttctgga actctaaggc ctcctccagc gacggcccca cctctcttcc
     5281 tgccagtgac gaagaccccg agggcgccgt cctgcccaag gacaagctta tctccctgtg
     5341 tcagtgcgac cccatcctca ggtggatgag gagctgcgac cacatcctct accaggcgct
     5401 ggtggagatt ctcatccccg acgtgctgag gccggtcccc agtaccttga cacaggccat
     5461 ccgtaacttt gccaagagct tggaaggctg gttgacaaat gccatgagtg acttcccaca
     5521 acaggtcatc cagaccaagg tgggcgtcgt cagtgccttc gcccagacgc tgcggcgcta
     5581 cacgtccctc aaccacctgg cgcaggcggc ccgggcggtg ctgcagaaca cgtcccagat
     5641 caaccagatg ctcagcgacc tcaaccgcgt ggactttgcc aacgtgcagg agcaggcctc
     5701 gtgggtgtgc cagtgcgagg agagtgtggt gcagcggctg gagcaggatt tcaagctgac
     5761 cctgcagcag cagagctccc tggaccagtg ggccagctgg ctggacagtg tggtcaccca
     5821 ggtcctgaag cagcatgccg gcagccccag cttccccaag gccgcccggc agttcttgct
     5881 gaaatggtcc ttttacagct ccatggtgat ccgggacctg accctgcgca gcgctgccag
     5941 cttcggctcc ttccacctca tccgcctgct ctacgacgag tacatgttct acctggtgga
     6001 gcaccgcgtc gcggaggcca ccggagagac gccgatcgct gtgatgggag agttcaacga
     6061 tctcgcctct ctgtcgctga cgctgctcga caaagatgac atgggcgatg agcagcgtgg
     6121 cagcgaggcg ggcccagacg cccgcagcct gggtgagccc ctggtaaagc gggagcgcag
     6181 tgaccccaac cactccctgc agggcatcta gtaatgaact agtccgtaac ttgaaagtat
     6241 ttcgatttct tggctttata tatcttgtgg aaaggacgag ttgcagtatc tgagagactg
     6301 cttacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6361 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6421 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6481 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6541 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6601 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6661 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6721 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6781 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6841 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6901 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6961 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     7021 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7081 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7141 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7201 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     7261 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7321 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7381 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7441 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7501 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7561 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     7621 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7681 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7741 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     7801 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7861 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7921 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7981 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8041 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8101 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8161 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8221 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     8281 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8341 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8401 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8461 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8521 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8581 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8641 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8701 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8761 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8821 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8881 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8941 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9001 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9061 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9121 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9181 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9241 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9301 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9361 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9421 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9481 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9541 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9601 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9661 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9721 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9781 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9841 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9901 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9961 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10021 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10081 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10141 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    10201 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    10261 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    10321 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    10381 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    10441 aacaaaagct ggagctgcaa gc
//
