LOCUS       sequence-39734-e         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM
  TITLE     The Brd4 Extraterminal Domain Confers Transcription Activation 
            Independent of pTEFb by Recruiting Multiple Proteins, Including 
            NSD3.
  JOURNAL   Mol Cell Biol. 2011 Jul . 31(13):2641-52.
  PUBMED    21555454
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2011 Jul . 31(13):2641-52."
COMMENT     Sequence Label: p6346 MSCV-CMV-Flag-HA-Brd4-1-444
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..46
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     56..80
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      153..162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             162..185
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             198..224
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    240..264
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcgactctcc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
       61 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
      121 gacaccggga ccgatccagc ctccggggat ctgccaccat ggactacaag gatgacgatg
      181 acaagctcga tggaggatac ccatacgatg ttccagatta cgctgctcga ggttatcaaa
      241 caagtttgta caaaaaagca ggctccgcgg ccgccccctt caccatgtct gcggagagcg
      301 gccctgggac gagattgaga aatctgccag taatggggga tggactagaa acttcccaaa
      361 tgtctacaac acaggcccag gcccaacccc agccagccaa cgcagccagc accaaccccc
      421 cgcccccaga gacctccaac cctaacaagc ccaagaggca gaccaaccaa ctgcaatacc
      481 tgctcagagt ggtgctcaag acactatgga aacaccagtt tgcatggcct ttccagcagc
      541 ctgtggatgc cgtcaagctg aacctccctg attactataa gatcattaaa acgcctatgg
      601 atatgggaac aataaagaag cgcttggaaa acaactatta ctgga
//
