LOCUS       sequence-397424-       12391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0594
FEATURES             Location/Qualifiers
     source          1..12391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8248..8836
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8301..8321)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8719..8730)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8908..9141
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9219..9340
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9256..9275)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9310..9329
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9380..9515
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9535..9554)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9536..9554)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9564..9581)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9564..9580)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9573..9595)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9722..10177
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9809..9828)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10019..10040
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10203..10307
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10308..10376,10377..11168)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10526..10545)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11339..11927
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11828..11847
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12081..12098
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12215..12236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12251..12256,12257..12274,12275..12281)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12289..12305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12294..12316
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12313..12329
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12313..12329
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12348..12368
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12350..12368
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaca aagaggaaga agaccatcaa
     4141 ccttaatata caagacgccc agaagaggac tgctctacac tgggcctgtg tcaatggcca
     4201 tgaggaagta gtaacatttc tggtagacag aaagtgccag cttgacgtcc ttgatggcga
     4261 acacaggaca cctctgatga aggctctaca atgccatcag gaggcttgtg caaatattct
     4321 gatagattct ggtgccgata taaatctcgt agatgtgtat ggcaacacgg ctctccatta
     4381 tgctgtttat agtgagattt tgtcagtggt ggcaaaactg ctgtcccatg gtgcagtcat
     4441 cgaagtgcac aacaaggcta gcctcacacc acttttacta tccataacga aaagaagtga
     4501 gcaaattgtg gaatttttgc tgataaaaaa tgcaaatgcg aatgcagtta ataagtataa
     4561 atgcacagcc ctcatgcttg ctgtatgtca tggatcatca gagatagttg gcatgcttct
     4621 tcagcaaaat gttgacgtct ttgctgcaga tatatgtgga gtaactgcag aacattatgc
     4681 tgttacttgt ggatttcatc acattcatga acaaattatg gaatatatac gaaaattatc
     4741 taaaaatcat caaaatacca atccagaagg aacatctgca ggaacacctg atgaggctgc
     4801 acccttggcg gaaagaacac ctgacacagc tgaaagcttg gtggaaaaaa cacctgatga
     4861 ggctgcaccc ttggtggaaa gaacacctga cacggctgaa agcttggtgg aaaaaacacc
     4921 tgatgaggct gcatccttgg tggagggaac atctgacaaa attcaatgtt tggagaaagc
     4981 gacatctgga aagttcgaac agtcagcaga agaaacacct agggaaatta cgagtcctgc
     5041 aaaagaaaca tctgagaaat ttacgtggcc agcaaaagga agacctagga agatcgcatg
     5101 ggagaaaaaa gaagacacac ctagggaaat tatgagtccc gcaaaagaaa catctgagaa
     5161 atttacgtgg gcagcaaaag gaagacctag gaagatcgca tgggagaaaa aagaaacacc
     5221 tgtaaagact ggatgcgtgg caagagtaac atctaataaa actaaagttt tggaaaaagg
     5281 aagatctaag atgattgcat gtcctacaaa agaatcatct acaaaagcaa gtgccaatga
     5341 tcagaggttc ccatcagaat ccaaacaaga ggaagatgaa gaatattctt gtgattctcg
     5401 gagtctcttt gagagttctg caaagattca agtgtgtata cctgagtcta tatatcaaaa
     5461 agtaatggag ataaatagag aagtagaaga gcctcctaag aagccatctg ccttcaagcc
     5521 tgccattgaa atgcaaaact ctgttccaaa taaagccttt gaattgaaga atgaacaaac
     5581 attgagagca gatccgatgt tcccaccaga atccaaacaa aaggactatg aagaaaattc
     5641 ttgggattct gagagtctct gtgagactgt ttcacagaag gatgtgtgtt tacccaaggc
     5701 tacacatcaa aaagaaatag ataaaataaa tggaaaatta gaagagtctc ctaataaaga
     5761 tggtcttctg aaggctacct gcggaatgaa agtttctatt ccaactaaag ccttagaatt
     5821 gaaggacatg caaactttca aagcagagcc tccggggaag ccatctgcct tcgagcctgc
     5881 cactgaaatg caaaagtctg tcccaaataa agccttggaa ttgaaaaatg aacaaacatt
     5941 gagagcagat gagatactcc catcagaatc caaacaaaag gactatgaag aaaattcttg
     6001 ggatactgag agtctctgtg agactgtttc acagaaggat gtgtgtttac ccaaggctgc
     6061 gcatcaaaaa gaaatagata aaataaatgg aaaattagaa gggtctcctg ttaaagatgg
     6121 tcttctgaag gctaactgcg gaatgaaagt ttctattcca actaaagcct tagaattgat
     6181 ggacatgcaa actttcaaag cagagcctcc cgagaagcca tctgccttcg agcctgccat
     6241 tgaaatgcaa aagtctgttc caaataaagc cttggaattg aagaatgaac aaacattgag
     6301 agcagatgag atactcccat cagaatccaa acaaaaggac tatgaagaaa gttcttggga
     6361 ttctgagagt ctctgtgaga ctgtttcaca gaaggatgtg tgtttaccca aggctacgca
     6421 tcaaaaagaa atagataaaa taaatggaaa attagaagag tctcctgata atgatggttt
     6481 tctgaaggct ccctgcagaa tgaaagtttc tattccaact aaagccttag aattgatgga
     6541 catgcaaact ttcaaagcag agcctcccga gaagccatct gccttcgagc ctgccattga
     6601 aatgcaaaag tctgttccaa ataaagcctt ggaattgaag aatgaacaaa cattgagagc
     6661 agatcagatg ttcccttcag aatcaaaaca aaagaaggtt gaagaaaatt cttgggattc
     6721 tgagagtctc cgtgagactg tttcacagaa ggatgtgtgt gtacccaagg ctacacatca
     6781 aaaagaaatg gataaaataa gtggaaaatt agaagattca actagcctat caaaaatctt
     6841 ggatacagtt cattcttgtg aaagagcaag ggaacttcaa aaagatcact gtgaacaacg
     6901 tacaggaaaa atggaacaaa tgaaaaagaa gttttgtgta ctgaaaaaga aactgtcaga
     6961 agcaaaagaa ataaaatcac agttagagaa ccaaaaagtt aaatgggaac aagagctctg
     7021 cagtgtgaga ttgactttaa accaagaaga agagaagaga agaaatgccg atatattaaa
     7081 tgaaaaaatt agggaagaat taggaagaat cgaagagcag cataggaaag agttagaagt
     7141 gaaacaacaa cttgaacagg ctctcagaat acaagatata gaattgaaga gtgtagaaag
     7201 taatttgaat caggtttctc acactcatga aaatgaaaat tatctcttac atgaaaattg
     7261 catgttgaaa aaggaaattg ccatgctaaa actggaaata gccacactga aacaccaata
     7321 ccaggaaaag gaaaataaat actttgagga cattaagatt ttaaaagaaa agaatgctga
     7381 acttcagatg accctaaaac tgaaagagga atcattaact aaaagggcat ctcaatatag
     7441 tgggcagctt aaagttctga tagctgagaa cacaatgctc acttctaaat tgaaggaaaa
     7501 acaagacaaa gaaatactag aggcagaaat tgaatcacac catcctagac tggcttctgc
     7561 tgtacaagac catgatcaaa ttgtgacatc aagaaaaagt caagaacctg ctttccacat
     7621 tgcaggagat gcttgtttgc aaagaaaaat gaatgttgat gtgagtagta cgatatataa
     7681 caatgaggtg ctccatcaac cactttctga agctcaaagg aaatccaaaa gcctaaaaat
     7741 taatctcaat tatgcaggag atgctctaag agaaaataca ttggtttcag aacatgcaca
     7801 aagagaccaa cgtgaaacac agtgtcaaat gaaggaagct gaacacatgt atcaaaacga
     7861 acaagataat gtgaacaaac acactgaaca gcaggagtct ctagatcaga aattatttca
     7921 actacaaagc aaaaatatgt ggcttcaaca gcaattagtt catgcacata agaaagctga
     7981 caacaaaagc aagataacaa ttgatattca ttttcttgag aggaaaatgc aacatcatct
     8041 cctaaaagag aaaaatgagg agatatttaa ttacaataac catttaaaaa accgtatata
     8101 tcaatatgaa aaagagaaag cagaaacaga aaactcatag taatgaacta gtccgtaact
     8161 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaag atatccggat
     8221 gtgcagaagc ggacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     8281 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8341 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8401 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8461 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8521 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8581 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8641 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8701 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8761 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8821 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     8881 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     8941 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9001 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9061 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9121 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     9181 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     9241 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9301 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     9361 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9421 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9481 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     9541 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     9601 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     9661 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     9721 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9781 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9841 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9901 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9961 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
    10021 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    10081 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    10141 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
    10201 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    10261 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    10321 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10381 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    10441 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    10501 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    10561 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    10621 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    10681 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    10741 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    10801 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    10861 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    10921 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    10981 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    11041 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    11101 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    11161 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    11221 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    11281 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11341 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11401 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11461 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    11521 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11581 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11641 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    11701 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    11761 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    11821 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    11881 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    11941 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    12001 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    12061 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    12121 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    12181 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    12241 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    12301 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    12361 actaaaggga acaaaagctg gagctgcaag c
//
