LOCUS       sequence-397588-        4386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector for mosTI(unc-119, wrmScarlet). MCS or Golden Gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4386)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH45 - MosTI [unc-119(partial) | MCS | wrmSc...
FEATURES             Location/Qualifiers
     source          1..4386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1633..1649)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1633..1649)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1646..1668)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1821..2409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1901..1920)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2526..2550)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2650..3441,3442..3510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3273..3292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3511..3615)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3714..3736
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3728..3745
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3729..3745
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4369..4386,1..16))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cattatacga agttatgata tcaaaaatga ttacgccaag ctgtaagttt aaacatgatc
       61 ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga aatcactcac
      121 aacgatggat acgctaatgc cagagacgag tgcacagcta gcaataccga gctcactgca
      181 gacgtctcta aaaatggggc ccgtttcaaa gggagaggcc gttataaaag aatttatgcg
      241 tttcaaggtc cacatggagg gatccatgaa cggacacgag ttcgagatcg agggagaggg
      301 agagggacgt ccatacgagg gaacccaaac cgccaaggtg agttttatta tcgattttcg
      361 ggattttcta cttgtaatta ccttttgttt ttaataagtt gcttttttcc agctcaaggt
      421 caccaaggga ggaccactcc cattctcctg ggacatcctc tccccacaat tcatgtacgg
      481 atcccgtgcc ttcaccaagc acccagccga catcccagac tactacaagc aatccttccc
      541 agagggattc aagtgggagc gtgtcatgaa cttcgaggac ggaggagccg tcaccgtcac
      601 ccaaggtagg ttttcagttt caatttttgt tgcaactacc tcaactctat gttttcagac
      661 acctccctcg aggacggaac cctcatctac aaggtcaagc tccgtggaac caacttccca
      721 ccagacggac cagtcatgca aaagaagacc atgggatggg aggcctccac cgagcgactc
      781 tacccagagg acggagtcct caagggagac atcaaggtaa atttttcaag cttctaaatt
      841 ccaaaataaa ccattttaat ttcttaattc cagatggccc tccggctcaa ggacggagga
      901 cgttacctcg ccgacttcaa gaccacctac aaggccaaga agccagtcca aatgccagga
      961 gcctacaacg tcgaccgtaa gctcgacatc acctcccaca acgaggacta caccgtcgtc
     1021 gagcaatacg agcgttccga gggacgtcac tccaccggag gaatggacga gctctacaag
     1081 cccgggtgat ggtctctgag ctatgcaaaa tcctttcaag cattcccttc ttctctatca
     1141 ctcttctttc tttttgtcaa aaaattctct cgctaattta tttgcttttt taatgttatt
     1201 attttatgac tttttatagt cactgaaaag tttgcatctg agtgaagtga atgctatcaa
     1261 aatgtgattc tgatatccag gaaacggaga atctgtgcgg caatgtctta tcatttgaaa
     1321 cgcgccgtat cgatgctgac atcatgcggc tcccgtgtta gtcgagaaga tgggtacggg
     1381 cctttattca tccaacactc tacggcttct tcaagagcta gtagggcacc ctgtagttgg
     1441 aaagggatta tttcgtatgg cgagcccata ccgtctttct tgcggaagct taacacgata
     1501 ggaagttgga tagtttcgaa cgatggttcc atacggaacg ctgtctcaag gatgagtctg
     1561 acggagtgta actcgaaggc taggtggagg ctcagtgatg ataagtctgc gatggtggat
     1621 gcatgtgtca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca gagggcacaa
     1681 tcctattccg cgctatccga caatctccaa gacattaggt ggagttcagt tcggcgtatg
     1741 gcatatgtcg ctggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1801 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1861 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1921 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1981 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2041 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2101 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2161 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2221 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2281 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2341 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2401 ggatctcaag aagatccttt gatcttttct acggggtctg acgctctatt caacaaagcc
     2461 gccgtcccgt caagtcagcg taaatgggta gggggcttca aatcgtcctc gtgataccaa
     2521 ttcggagcct gcttttttgt acaaacttgt tgataatggc aattcaagga tcttcaccta
     2581 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2641 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2701 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2761 atctggcccc agtgctgcaa tgataccgcg agagccacgc tcaccggctc cagatttatc
     2821 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2881 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2941 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3001 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3061 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3361 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3601 aataggggtt ccgcgcacat ttccccgaaa agtgccagat acctgaaaca aaacccatcg
     3661 tacggccaag gaagtctcca ataactgtga tccaccacaa gcgccagggt tttcccagtc
     3721 acgacgttgt aaaacgacgg ccagtcatgc ataatccgca cgcatctgga ataaggaagt
     3781 gccattccgc ctgacctaag tgcttttcga gatcgccaaa ccggagaacg atcaggagaa
     3841 tgatgagtcg ccacaggagt cggcaagata cgtgcgttat agatttgctc caaacttttt
     3901 gaaactcaaa acggtcggag caactgtgga attcaaagta ggagacatcc caatccatca
     3961 tttccgaatg atcgaacgtc acttcttcaa agatcgcctt ctgaagtgtt ttgactttga
     4021 attcggattc tgtattccga attcacgaaa caactgtgaa catatctatg agttccctca
     4081 actctctcaa caactcagtg agtcattatt ctaaaaagta caattcaaaa gactaatctc
     4141 tttttcagtg gacgacatga tcaacaatcc aaacgagact cgttctgaca gcttctattt
     4201 cgtcgataac aaactcgtca tgcacaacaa agccgactac tcatatgatg cataaatctt
     4261 cattgttgag tttatcttgt tgatttttga ataaattatc aactctttac tttttaatgg
     4321 gttatgaaat aaataaacat tgaaaactga taaacaacgt tcatctctat aacttcgtat
     4381 agcata
//
