LOCUS       sequence-397647-       12093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A Cre recombinase that is expressed upon doxycycline when combined 
            with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 
            is a cre recombinase that is activated by violet light..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12093)
  AUTHORS   Vizoso M, E J Pritchard C, Bombardelli L, van den Broek B, 
            Krimpenfort P, Beijersbergen RL, Jalink K, van Rheenen J
  TITLE     A doxycycline- and light-inducible Cre recombinase mouse model for 
            optogenetic genome editing.
  JOURNAL   Nat Commun. 2022 Oct 28;13(1):6442. doi: 10.1038/s41467-022-33863-z.
  PUBMED    36307419
REFERENCE   2  (bases 1 to 12093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-28"; volume: "13(1):6442. doi"; pages: " 
            10.1038/s41467-022-33863-z"
COMMENT     Sequence Label: DiLiCre 2.0
FEATURES             Location/Qualifiers
     source          1..12093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2393..2742
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    2401..2419
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2437..2455
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2473..2491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2509..2527
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2545..2563
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2581..2599
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2617..2635
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2681..2705
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2773..2782
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2779..3807
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             3814..4539
                     /codon_start=1
                     /product="photocleavable protein that separates into two 
                     fragments upon exposure to violet light (Zhang et al., 
                     2017)"
                     /label=PhoCl
                     /translation="VIPDYFKQSFPEGYSWERSMTYEDGGICIATNDITMEGDSFINKI
                     HFKGTNFPPNGPVMQKRTVGWEASTEKMYERDGVLKGDVKMKLLLKGGGHYRCDYRTTY
                     KVKQKPVKLPDYHFVDHRIEILSHDKDYNKVKLYEHAVARNSTDSMDELYKGGSGGMVS
                     KGEETITSVIKPDMKNKLRMEGNVNGHAFVIEGEGSGKPFEGIQTIDLEVKEGAPLPFA
                     YDILTTAFHYGNRVFTKYPR"
     CDS             4555..5490
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEEA
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5524..6459
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5572..6465
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     misc_feature    6548..7136
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6601..6621)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7019..7030)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7139..7155)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7140..7156)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7661..7841
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7867..7884)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7873..8097
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8143..8571
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8230..8249)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8440..8461
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8580..8600)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8585..8914
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8765..8900
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8827..8846
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8962..9009
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9028..9402
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9532..9653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9569..9588)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9623..9642
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9702..9718)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9702..9718)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9715..9737)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9726..9742
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9750..9756,9757..9774,9775..9780))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9795..9816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9933..9950)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10104..10692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10184..10203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10863..11654,11655..11723))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11486..11505
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11724..11828)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11903..11922)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttagctagct aggggccctt tcgtcttcac tcgagtttac
     2401 tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat agagaacgta
     2461 tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc cctatcagtg
     2521 atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc tacagtttac
     2581 tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat agagaacgta
     2641 tgtcgaggta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta gtgaaccgtc
     2701 agatcgcctg gagcaattcc acaacacttt tgtcttatac ttacgcgtct gcaggatatc
     2761 aagttaatta atgccaccat ggccaattta ctgaccgtac accaaaattt gcctgcatta
     2821 ccggtcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc
     2881 caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca
     2941 tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat
     3001 cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag
     3061 ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca
     3121 ctggttatgc ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct
     3181 ctagcgttcg aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc
     3241 tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata
     3301 gccgaaattg ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta
     3361 atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc
     3421 ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg
     3481 aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc
     3541 cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc
     3601 gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga
     3661 gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc
     3721 tggaccaatg taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca
     3781 atggtgcgcc tgctggaaga tggcgattcc ggagtgatcc ctgactactt caagcagagc
     3841 ttccccgagg gctacagctg ggagcgcagc atgacctacg aggacggcgg catctgcatc
     3901 gccaccaacg acatcacaat ggagggggac agcttcatca acaagatcca cttcaagggc
     3961 acgaacttcc cccccaacgg ccccgtgatg cagaagagga ccgtgggctg ggaggccagc
     4021 accgagaaga tgtacgagcg cgacggcgtg ctgaagggcg acgtgaagat gaagctgctg
     4081 ctgaagggcg gcggccacta tcgctgcgac taccgcacca cctacaaggt caagcagaag
     4141 cccgtaaagc tgcccgacta ccacttcgtg gaccaccgca tcgagatcct gagccacgac
     4201 aaggactaca acaaggtgaa gctgtacgag cacgccgtgg cccgcaactc caccgacagt
     4261 atggacgagc tgtacaaggg tggcagcggt ggcatggtga gcaagggcga ggagaccatt
     4321 acaagcgtga tcaagcctga catgaagaac aagctgcgca tggagggcaa cgtgaacggc
     4381 cacgccttcg tgatcgaggg cgagggcagc ggcaagccct tcgagggcat ccagacgatt
     4441 gatttggagg tgaaggaggg cgccccgctg cccttcgcct acgacatcct gaccaccgcc
     4501 ttccactacg gcaaccgcgt gttcaccaag tacccacggg gcagcggcag cggcgctgga
     4561 gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac
     4621 agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc
     4681 cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc
     4741 ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg
     4801 ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta
     4861 gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt
     4921 gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc
     4981 ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag
     5041 tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc
     5101 accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac
     5161 actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg
     5221 gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat
     5281 ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggaggcggcg
     5341 gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga ggaggcggac
     5401 caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc
     5461 acgggggagg cagagggttt ccctgccaca gctggcagcg gcagcggcgg cggcggcgcg
     5521 ccggctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct
     5581 aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat
     5641 gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg
     5701 atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg
     5761 aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct tctagaatgt
     5821 gcctggctag agatcctgat gattggtctc gtctggcgct ccatggagca cccagtgaag
     5881 ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg
     5941 gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag
     6001 ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt
     6061 ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag
     6121 atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac
     6181 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc
     6241 atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg
     6301 gaggcggcgg acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag
     6361 gagacggacc aaagccactt ggccactgcg ggctctactt catcgcattc cttgcaaaag
     6421 tattacatca cgggggaggc agagggtttc cctgccacag cttgatagcg gccgctaacg
     6481 atgtgctgag ggcagtcccc gatctcgtgc ttgcccaaat cccgaattcg atatcaagct
     6541 tatcggtaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6601 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6661 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6721 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6781 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6841 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6901 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     6961 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7021 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7081 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     7141 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     7201 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     7261 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7321 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     7381 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     7441 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     7501 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     7561 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     7621 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     7681 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7741 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7801 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     7861 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     7921 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     7981 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     8041 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     8101 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     8161 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     8221 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8281 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     8341 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     8401 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     8461 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     8521 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     8581 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8641 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8701 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8761 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8821 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     8881 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     8941 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     9001 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     9061 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     9121 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     9181 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     9241 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     9301 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     9361 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     9421 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     9481 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     9541 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9601 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9661 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     9721 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     9781 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     9841 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     9901 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     9961 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    10021 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10081 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10141 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10201 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    10261 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10321 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    10381 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10441 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10501 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    10561 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    10621 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    10681 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    10741 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    10801 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    10861 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    10921 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    10981 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    11041 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    11101 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    11161 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    11221 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    11281 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    11341 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    11401 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    11461 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    11521 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    11581 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    11641 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    11701 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    11761 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    11821 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
    11881 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
    11941 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
    12001 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
    12061 tgcttcgcga tgtacgggcc agatatacgc gtt
//
