LOCUS       sequence-397661-       14110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0125
FEATURES             Location/Qualifiers
     source          1..14110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    9967..10555
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10020..10040)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10438..10449)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10627..10860
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10938..11059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10975..10994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11029..11048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11099..11234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11254..11273)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11255..11273)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11283..11300)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11283..11299)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11292..11314)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11441..11896
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11528..11547)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11738..11759
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11922..12026
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12027..12095,12096..12887)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12245..12264)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13058..13646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13547..13566
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13800..13817
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13934..13955
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13970..13975,13976..13993,13994..14000)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14008..14024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14013..14035
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14032..14048
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14032..14048
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14067..14087
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14069..14087
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gacggaaagg gagacgccgc
     4141 cgctgtcgcc ggggctgggg ctgaggctcc ggcggtagcg ggagccggag atggagtcga
     4201 gactgagtcc atggttcggg gtcatcgccc cgtatctcca gcgccgggag cctcgggact
     4261 gcggccgtgt ctgtggcagc tggagacaga gctgagggag caagaggtgt cggaggtctc
     4321 atctttgaac tactgccgga gcttctgcca gaccttattg caatatgcaa gcaacaagaa
     4381 tgcatcagaa catattgtgt atcttctgga ggtatatcga cttgccatcc aaagctttgc
     4441 cagtgcacgt ccatacttaa ctactgaatg tgaagatgtc ctcttagtgc ttggcagatt
     4501 agtactgagt tgtttcgaat tactgctttc agtgtctgaa agtgaactgc catgtgaagt
     4561 ctggctacca ttccttcagt ctctacagga gtcacatgat gcattattgg aatttgggaa
     4621 taataaccta caaatattgg ttcatgttac caaggaaggg gtgtggaaaa acccagttct
     4681 tcttaaaatt ctgtctcaac agccagtaga aacggaggaa gtcaataaat tgattgcaca
     4741 agaaggacct tcctttctgc aaatgcgaat aaaacatttg ttgaaatcta actgcatccc
     4801 ccaggctact gctttatcaa aactatgtgc agaatctaaa gaaatttcaa atgtgtcatc
     4861 ttttcagcaa gcctatatca catgtttatg ttctatgctc cctaatgaag atgctattaa
     4921 ggagattgca aaggtcgact gcaaggaagt actagacatc atttgtaatc tggaatctga
     4981 ggggcaggat aacacagcat ttgttctttg tacgacttac cttacccagc agctccaaac
     5041 tgcaagtgta tattgttctt gggaactgac tcttttttgg agtaaactgc aaagaagaat
     5101 tgacccttct ttagatactt ttttggagcg ctgtcgtcag tttggtgtca tagctaaaac
     5161 gcagcagcat ttattttgcc tcattagagt tatacaaact gaagcacaag atgctggtct
     5221 tggggtgtca attttactgt gtgtcagagc tcttcaactc agatcaagtg aagatgagga
     5281 aatgaaggca tcagtttgta aaacaattgc ctgtctttta ccagaagatt tagaagttag
     5341 acgagcctgt cagcttacag aattcttaat tgaacccagt ttggatggat ttaatatgtt
     5401 agaagaacta tatttgcaac cagatcaaaa atttgatgaa gaaaatgcac cggttccaaa
     5461 ttctcttcga tgtgagctct tactagcttt aaaagcccac tggccttttg atcctgagtt
     5521 ttgggactgg aaaactttaa aacgacactg ccaccaactt ttaggacaag aagcctcaga
     5581 ttctgatgat gatttaagtg gctatgaaat gtccattaat gacacagatg ttttagagtc
     5641 atttctcagt gactatgatg agggtaaaga agataaacaa tatagaagaa gagatttgac
     5701 agatcagcat aaggagaaaa gagacaaaaa acctattggc tcttctgaaa gatatcagag
     5761 gtggcttcag tacaagtttt tctgtttgtt atgtaagcgg gaatgtatag aggctagaat
     5821 tcttcatcat tctaagatgc atatggaaga tggaatttac acctgtccag tttgtattaa
     5881 aaaatttaag agaaaagaaa tgtttgttcc tcatgtgatg gagcatgtta aaatgccacc
     5941 aagcagaagg gaccgctcta aaaagaaatt actgttaaaa ggctctcaaa agggtatttg
     6001 tcctaagagc ccctctgcaa tcccagagca aaaccattca ttgaatgacc aagccaaagg
     6061 agagtctcat gaatatgtca cattcagcaa attagaagat tgccacctgc aagacagaga
     6121 tttgtatcca tgtcccggta cagactgttc ccgtgtgttt aagcaattta aatacttaag
     6181 tgtgcatctt aaagctgaac accaaaataa tgatgaaaat gccaagcact acttggatat
     6241 gaaaaataga agagagaagt gtacttactg tcgacgacat tttatgtctg cttttcacct
     6301 tcgagagcac gaacaagtgc attgtgggcc tcagccttat atgtgtgtat ctatagattg
     6361 ctatgctagg tttggatcag taaatgaact acttaaccat aaacaaaagc atgacgatct
     6421 gcgttacaaa tgtgaattaa atggctgtaa tattgttttc agtgacttgg gacagcttta
     6481 ccaccatgaa gcacaacact ttagggatgc atcttacaca tgcaacttcc ttggctgtaa
     6541 aaagttctat tactccaaaa ttgaatacca gaatcacctc tcaatgcata atgttgaaaa
     6601 ttcaaatgga gacataaaga aatcagtgaa acttgaggag tctgcaacag gtgaaaagca
     6661 agattgtatt aatcagcccc atctacttaa ccaaactgat aaatcacatt tacctgaaga
     6721 tcttttctgt gcagaatcag ctaattctca aatagataca gaaactgcag aaaacctgaa
     6781 agaaaacagt gacagtaatt ctagtgatca gttaagtcat agctcttcag cttcaatgaa
     6841 tgaagagcta attgacacac tagatcactc tgaaactatg caggatgtat tgttatctaa
     6901 tgagaaagtc tttgggccct ccagtttaaa agaaaaatgt tccagtatgg cagtttgttt
     6961 tgacgggact aagtttacct gtggttttga tggctgtggt tccacataca aaaatgcaag
     7021 aggaatgcag aaacatttac ggaaggttca tccataccat ttcaagccca aaaagataaa
     7081 gacgaaagat ctgtttccct ctttgggtaa tgaacataat cagacaactg aaaagttgga
     7141 tgcagaacct aaaccctgct cagatacaaa cagtgactcc ccagatgaag gtctagatca
     7201 caatattcac attaaatgta aacgagaaca tcaaggttat tcctcagaat cctccatttg
     7261 tgcttctaaa aggccctgta cagaggatac catgttggaa cttctgttac gcttgaaaca
     7321 tttaagcttg aaaaactcaa taacacatgg atctttctca gggtcattgc aggggtaccc
     7381 atccagtggt gctaagtctc ttcagtcagt ttcatctatc tcagacctta attttcagaa
     7441 tcaagatgaa aacatgccaa gtcagtacct tgcacagttg gcggctaagc cgtttttctg
     7501 tgagcttcaa ggatgcaaat atgaatttgt gaccagagag gctctgttaa tgcattatct
     7561 taaaaagcat aattattcaa aagaaaaagt ccttcagtta accatgttcc aacatcggta
     7621 ttccccattt cagtgtcata tttgccaaag gtcatttaca agaaaaacac accttaggat
     7681 tcattataaa aataaacatc aaattggcag tgacagagca actcacaaac tattagataa
     7741 tgaaaagtgt gatcatgaag gcccatgttc agtagatagg ttgaaaggtg attgttctgc
     7801 agaacttgga ggtgatccca gtagtaactc tgagaaacca cactgtcatc ctaaaaagga
     7861 tgaatgtagt tctgaaacag atttggaatc atcttgtgaa gaaacagaaa gtaaaacatc
     7921 tgacatttca tcaccaatag gcagccatag agaagaacaa gaaggaagag agggcagagg
     7981 tagcaggcga actgttgcta aaggaaatct gtgttatatt ttgaataaat accacaaacc
     8041 attccattgt attcataaaa cttgcaactc ctcattcacc aatctaaaag gcttaattcg
     8101 ccattacaga actgtacatc agtacaacaa agaacagtta tgtttggaga aagacaaagc
     8161 aagaaccaaa agggaacttg tcaaatgtaa aaagatattt gcttgcaaat ataaggaatg
     8221 taataaacgc ttcctgtgtt ccaaagctct tgctaagcac tgtagtgatt ctcataacct
     8281 agaccatatt gaagagccta aagtactttc cgaagctgga tctgcagcaa ggttttcttg
     8341 taaccagcct cagtgccctg ctgtttttta tacattcaac aagttgaagc accacttgat
     8401 ggaacagcat aatattgaag gggaaataca ttcagattat gaaattcatt gtgatcttaa
     8461 tggctgtggc cagattttca cccatcgcag taattactca caacatgtat attaccgaca
     8521 taaagactat tatgatgatt tgtttagaag ccagaaagta gcaaatgaga gactactaag
     8581 gagtgaaaag gtatgtcaaa cagctgatac tcaggggcat gaacatcaga ccaccaggag
     8641 atcatttaat gctaagtcta aaaaatgtgg cttaatcaaa gaaaagaaag ccccaataag
     8701 ttttaaaacc agagctgagg ccctccatat gtgtgtggag cactctgagc acacacagta
     8761 cccctgcatg gttcaaggat gcttatctgt ggtgaagttg gagagcagca ttgtgaggca
     8821 ttacaaacgc actcatcaga tgagtagtgc ctatttagag caacagatgg agaatcttgt
     8881 tgtttgcgtt aagtacggta ccaaaattaa ggaggaaccc ccttctgaag cagatccctg
     8941 tataaagaaa gaagaaaata gaagctgtga atcagagcgc acagaacaca gccattcccc
     9001 gggtgacagt agtgcaccca tccagaacac tgattgctgt cattcaagtg aaagggatgg
     9061 aggtcagaaa gggtgcatag aaagcagctc agtatttgat gcagatactc tgctctacag
     9121 gggaactttg aaatgtaatc atagttccaa aaccacttcc ctagaacagt gtaatatagt
     9181 tcagcctcct cctccttgta aaatagaaaa ttccatacct aatcccaatg ggactgaaag
     9241 tgggacttat ttcacaagtt tccagctgcc tttaccaagg atcaaagaat cagaaactag
     9301 gcagcatagt tcagggcaag aaaacactgt aaaaaatcca acccatgtcc caaaagagaa
     9361 ttttaggaaa cattcacagc cccggtcatt tgatttgaag acttacaaac ctatgggatt
     9421 tgaatcttca tttctgaaat ttattcagga aagtgaagag aaagaagatg attttgatga
     9481 ttgggagcct tcagagcact taacattaag taattcttca cagtccagta atgatttaac
     9541 agggaatgtt gtggcaaata atatggtgaa tgacagtgaa cctgaagttg acatacctca
     9601 ttcttccagt gactctacaa ttcatgagaa cctgactgca atcccacctt taatagtagc
     9661 tgaaacaaca acagttcctt ccttggaaaa cctgagggtt gtattggaca aagcattaac
     9721 agactgtgga gagcttgcct taaaacagct tcattatctt cggccagtgg tggttcttga
     9781 aagatctaag ttttccacac caattttaga cttatttcca acaaaaaaga cagatgagct
     9841 ttgtgtagga agttcatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     9901 gctttatata tcttgtggaa aggacgagca cgggccgttt aaaggatacc aacgcgttaa
     9961 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
    10021 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
    10081 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
    10141 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
    10201 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
    10261 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
    10321 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
    10381 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    10441 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    10501 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
    10561 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
    10621 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
    10681 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    10741 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    10801 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    10861 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
    10921 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    10981 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    11041 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
    11101 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    11161 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    11221 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
    11281 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    11341 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    11401 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
    11461 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    11521 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    11581 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    11641 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    11701 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    11761 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    11821 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    11881 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    11941 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    12001 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    12061 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    12121 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    12181 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    12241 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    12301 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    12361 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    12421 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    12481 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    12541 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    12601 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    12661 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    12721 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    12781 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    12841 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    12901 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    12961 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    13021 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    13081 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    13141 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    13201 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    13261 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    13321 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    13381 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    13441 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    13501 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    13561 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    13621 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    13681 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    13741 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    13801 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    13861 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    13921 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    13981 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    14041 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    14101 agctgcaagc
//
