LOCUS       sequence-397907-       10105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1186
FEATURES             Location/Qualifiers
     source          1..10105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             complement(4933..4944)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    5962..6550
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6015..6035)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6433..6444)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6622..6855
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6933..7054
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6970..6989)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7024..7043
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7094..7229
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7249..7268)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7250..7268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7278..7295)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7278..7294)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7287..7309)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7436..7891
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7523..7542)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7733..7754
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7917..8021
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8022..8090,8091..8882)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8240..8259)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9053..9641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9542..9561
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9795..9812
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9929..9950
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9965..9970,9971..9988,9989..9995)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10003..10019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10008..10030
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10027..10043
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10027..10043
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10062..10082
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10064..10082
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtct acagcctctg ccgcctcctc
     4141 ctcctcctcg tcttcggccg gtgagatgat cgaagcccct tcccaggtcc tcaactttga
     4201 agagatcgac tacaaggaga tcgaggtgga agaggttgtt ggaagaggag cctttggagt
     4261 tgtttgcaaa gctaagtgga gagcaaaaga tgttgctatt aaacaaatag aaagtgaatc
     4321 tgagaggaaa gcgtttattg tagagcttcg gcagttatcc cgtgtgaacc atcctaatat
     4381 tgtaaagctt tatggagcct gcttgaatcc agtgtgtctt gtgatggaat atgctgaagg
     4441 gggctcttta tataatgtgc tgcatggtgc tgaaccattg ccatattata ctgctgccca
     4501 cgcaatgagt tggtgtttac agtgttccca aggagtggct tatcttcaca gcatgcaacc
     4561 caaagcgcta attcacaggg acctgaaacc accaaactta ctgctggttg caggggggac
     4621 agttctaaaa atttgtgatt ttggtacagc ctgtgacatt cagacacaca tgaccaataa
     4681 caaggggagt gctgcttgga tggcacctga agtttttgaa ggtagtaatt acagtgaaaa
     4741 atgtgacgtc ttcagctggg gtattattct ttgggaagtg ataacgcgtc ggaaaccctt
     4801 tgatgagatt ggtggcccag ctttccgaat catgtgggct gttcataatg gtactcgacc
     4861 accactgata aaaaatttac ctaagcccat tgagagcctg atgactcgtt gttggtctaa
     4921 agatccttcc cagcgccctt caatggagga aattgtgaaa ataatgactc acttgatgcg
     4981 gtactttcca ggagcagatg agccattaca gtatccttgt cagtattcag atgaaggaca
     5041 gagcaactct gccaccagta caggctcatt catggacatt gcttctacaa atacgagtaa
     5101 caaaagtgac actaatatgg agcaagttcc tgccacaaat gatactatta agcgcttaga
     5161 atcaaaattg ttgaaaaatc aggcaaagca acagagtgaa tctggacgtt taagcttggg
     5221 agcctcccgt gggagcagtg tggagagctt gcccccaacc tctgagggca agaggatgag
     5281 tgctgacatg tctgaaatag aagctaggat cgccgcaacc acaggcaacg gacagccaag
     5341 acgtagatcc atccaagact tgactgtaac tggaacagaa cctggtcagg tgagcagtag
     5401 gtcatccagt cccagtgtca gaatgattac tacctcagga ccaacctcag aaaagccaac
     5461 tcgaagtcat ccatggaccc ctgatgattc cacagatacc aatggatcag ataactccat
     5521 cccaatggct tatcttacac tggatcacca actacagcct ctagcaccgt gcccaaactc
     5581 caaagaatct atggcagtgt ttgaacagca ttgtaaaatg gcacaagaat atatgaaagt
     5641 tcaaacagaa attgcattgt tattacagag aaagcaagaa ctagttgcag aactggacca
     5701 ggatgaaaag gaccagcaaa atacatctcg cctggtacag gaacataaaa agcttttaga
     5761 tgaaaacaaa agcctttcta cttactacca gcaatgcaaa aaacaactag aggtcatcag
     5821 aagtcagcag cagaaacgac aaggcacttc atagtaatga actagtccgt aacttgaaag
     5881 tatttcgatt tcttggcttt atatatcttg tggaaaggac gactacgtga gccttaatgt
     5941 acgtggacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     6001 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6061 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6121 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6181 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6241 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6301 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     6361 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6421 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6481 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6541 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     6601 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     6661 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6721 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6781 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6841 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     6901 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     6961 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7021 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     7081 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7141 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7201 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     7261 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     7321 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     7381 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     7441 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7501 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7561 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7621 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7681 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7741 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7801 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7861 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     7921 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7981 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8041 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8101 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8161 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8221 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8281 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8341 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8401 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8461 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8521 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8581 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8641 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8701 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8761 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8821 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8881 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8941 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9001 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9061 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9121 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9181 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9241 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9301 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9361 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     9421 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9481 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9541 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     9601 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     9661 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     9721 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     9781 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     9841 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     9901 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     9961 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    10021 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    10081 gggaacaaaa gctggagctg caagc
//
