LOCUS       sequence-398071-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of a fusion of West Nile NS2B-NS3 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     The SARS-Cov-2 main protease (Mpro) is responsible for viral 
            polyprotein processing and therefore a therapeutic target.
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: WNV NS2B-NS3 protease (based on PDB 2YOL,
            cata...
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgac tgatatgtgg
      181 attgagcgta cagcagatat aacctgggag tctgacgccg aaatcactgg ttcatccgaa
      241 cgagtagacg ttcggttaga tgacgacggt aattttcagt tgatgaatga tccaggagcc
      301 ccctgggctg gcggtggagg atcaggtggg gggggaggcg gcgtgttgtg ggatactcca
      361 tcccctaaag aatataaaaa aggagatact actacggggg tttatcgtat aatgacaaga
      421 ggtcttctgg ggtcttatca agcaggagct ggtgttatgg tagaaggagt gtttcatacc
      481 ctctggcata ccacaaaggg cgcagctttg atgtcaggag aaggtcggct ggatccatat
      541 tggggatcgg tgaaagagga tcgtttatgt tatggcggac cgtggaaact gcagcacaag
      601 tggaatggtc atgatgaagt tcaaatgatc gtagttgaac ctgggaaaaa cgtaaaaaac
      661 gtgcaaacca aaccgggcgt gtttaaaacg cctgagggcg aaattggggc agtaacgttg
      721 gattatccca caggaacctc cggcagcccg attgtggata agaatggaga tgttattggc
      781 ttatatggca atggagtgat tatgccaaat ggcagttaca tatcagccat tgtgcagggt
      841 gagcgctaat ga
//
