LOCUS       sequence-398098-       11835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human PTPN2 transcript variant 
            3 under the spleen focus-forming virus (SFFV) promoter and Emerald 
            under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11835)
  TITLE     PTPN2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLTR-SFFV-PTPN2 transcript variant
            3-WPRE-UbC-...
FEATURES             Location/Qualifiers
     source          1..11835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(3587..3607)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4005..4016)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5055..5074
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5248..5257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5254..5256,5257..5259,5260..5973)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5299..5320)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5560..5579)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5907..5928
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6068..6301
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6326..6344)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6327..6344)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6634..6653)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6867..6886)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6986..7008
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7046..7064)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7132..7236
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7237..7305,7306..8097)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7455..7474)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8268..8856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8757..8776
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9010..9027
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9097..9117)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9102..9431
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9282..9417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10565..10630
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10760..10780
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11205..11339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11242..11261)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11296..11315
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11507..11835,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccgcca ccatgcccac caccatcgag cgggagttcg aagagttgga tactcagcgt
     2461 cgctggcagc cgctgtactt ggaaattcga aatgagtccc atgactatcc tcatagagtg
     2521 gccaagtttc cagaaaacag aaatcgaaac agatacagag atgtaagccc atatgatcac
     2581 agtcgtgtta aactgcaaaa tgctgagaat gattatatta atgccagttt agttgacata
     2641 gaagaggcac aaaggagtta catcttaaca cagggtccac ttcctaacac atgctgccat
     2701 ttctggctta tggtttggca gcagaagacc aaagcagttg tcatgctgaa ccgcattgtg
     2761 gagaaagaat cggttaaatg tgcacagtac tggccaacag atgaccaaga gatgctgttt
     2821 aaagaaacag gattcagtgt gaagctcttg tcagaagatg tgaagtcgta ttatacagta
     2881 catctactac aattagaaaa tatcaatagt ggtgaaacca gaacaatatc tcactttcat
     2941 tatactacct ggccagattt tggagtccct gaatcaccag cttcatttct caatttcttg
     3001 tttaaagtga gagaatctgg ctccttgaac cctgaccatg ggcctgcggt gatccactgt
     3061 agtgcaggca ttgggcgctc tggcaccttc tctctggtag acacttgtct tgttttgatg
     3121 gaaaaaggag atgatattaa cataaaacaa gtgttactga acatgagaaa ataccgaatg
     3181 ggtcttattc agaccccaga tcaactgaga ttctcataca tggctataat agaaggagca
     3241 aaatgtataa agggagattc tagtatacag aaacgatgga aagaactttc taaggaagac
     3301 ttatctcctg cctttgatca ttcaccaaac aaaataatga ctgaaaaata caatgggaac
     3361 agaataggtc tagaagaaga aaaactgaca ggtgaccgat gtacaggact ttcctctaaa
     3421 atgcaagata caatggagga gaacagtgag aggccaagat tgacagacac ctaagcggcc
     3481 gcgactctag agtcgacctg caggcatgca agcttgatat caagcttatc gataatcaac
     3541 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3601 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3661 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3721 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3781 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3841 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3901 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     3961 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4021 cggaccttcc ttcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa
     4081 gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca
     4141 taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt
     4201 gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt
     4261 ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac
     4321 gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt
     4381 gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg
     4441 tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc
     4501 tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg
     4561 ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa
     4621 ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa
     4681 agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt
     4741 cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt
     4801 tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt
     4861 tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct
     4921 ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga
     4981 cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta
     5041 tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt
     5101 ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca
     5161 gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacga
     5221 agcttggtac cgagctcgga tcaattcgcc accatggtga gcaagggcga ggagctgttc
     5281 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     5341 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     5401 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
     5461 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     5521 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     5581 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     5641 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     5701 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
     5761 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     5821 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     5881 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     5941 atcactctcg gcatggacga gctgtacaag taactcgagg gaattaattc gagctcggta
     6001 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6061 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     6121 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6181 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6241 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6301 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
     6361 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
     6421 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
     6481 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
     6541 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
     6601 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     6661 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     6721 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     6781 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     6841 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     6901 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     6961 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     7021 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     7081 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     7141 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7201 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7261 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7321 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7381 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7441 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     7501 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7561 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7621 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7681 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7741 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7801 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7861 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7921 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7981 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8041 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8101 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8161 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8221 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8281 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8341 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8401 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8461 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8521 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8581 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8641 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8701 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8761 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8821 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8881 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8941 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     9001 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     9061 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg
     9121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     9181 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     9241 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     9301 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     9361 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     9421 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt
     9481 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
     9541 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
     9601 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
     9661 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
     9721 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg
     9781 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     9841 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     9901 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     9961 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
    10021 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
    10081 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
    10141 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
    10201 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
    10261 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
    10321 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc
    10381 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
    10441 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa
    10501 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
    10561 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
    10621 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
    10681 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
    10741 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
    10801 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
    10861 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
    10921 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
    10981 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
    11041 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
    11101 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
    11161 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
    11221 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    11281 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    11341 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
    11401 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    11461 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
    11521 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
    11581 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
    11641 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
    11701 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
    11761 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
    11821 tacttcaaga actgc
//
