LOCUS       sequence-39824-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Duennwald ML, Jagadish S, Muchowski PJ, Lindquist S
  TITLE     Flanking sequences profoundly alter polyglutamine toxicity in yeast.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):11045-50.
  PUBMED    16832050
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):11045-50."
COMMENT     Sequence Label: MET FLAG46QDProCFPp303
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(226..249)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(265..281)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(265..281)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(282..631)
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
ORIGIN
        1 gtcttctcct ttactcaggg atcccccggg ctgcagttgt tgctgttgct gctgttgttg
       61 ctgttgctgc tgttgttgct gttgctgctg ttgttgctgt tgctgctgtt gctgctgttg
      121 ttgctgttgc tgctgttgtt gctgttgctg ctgttgttgc tgttgctgct gttggaagct
      181 tttgagggac tcgaaggcct tcatcagctt ttccagggtc gccagcttgt catcgtcgtc
      241 cttgtagtcc atggctctag gggggatcca ctagttctag agtatggatg ggggtaatag
      301 aattgtatct atgtatctga cgaccctgta ttacacgaag gaaatagtag atgaaaagac
      361 aagagagcaa gaaaaaggaa aaacttcctt tctaacagac gctttactta accttatata
      421 tatcttattt ttttcatcga gcgtgttcaa ttggacaagg tgccaccttt tcgacacttc
      481 ggttattatg ttacacagtt ttcatgagga tggcgccttg actaacttaa ttagtcattt
      541 gccaaccacc acagttcccc aatatcgaca gcttcacgtg ccatttgcca ttttgcgatc
      601 atgtgacctc aagagaaaaa gcaaaaaata atgagagcta agggattcga acccttgcat
      661 ccggggggga ac
//
