LOCUS       sequence-398286-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a guide RNA targeting BIRC6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Cervia LD, Shibue T, Borah AA, Gaeta B, He L, Leung L, Li N, Moyer 
            SM, Shim BH, Dumont N, Gonzalez A, Bick NR, Kazachkova M, Dempster 
            JM, Krill-Burger JM, Piccioni F, Udeshi ND, Olive ME, Carr SA, Root 
            DE, McFarland JM, Vazquez F, Hahn WC
  TITLE     A Ubiquitination Cascade Regulating the Integrated Stress Response 
            and Survival in Carcinomas.
  JOURNAL   Cancer Discov. 2023 Mar 1;13(3):766-795. doi: 
            10.1158/2159-8290.CD-22-1230.
  PUBMED    36576405
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1158/2159-8290";
            journalName: "Cancer Discov"; date: "2023-03-1- 1"; volume: "13"; 
            issue: "3"; pages: "766-795"
COMMENT     Sequence Label: pXPR_003-sgBIRC6-4
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..34
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    151..172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(187..192,193..210,211..217)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    225..241
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     230..252
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     249..265
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     249..265
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     284..304
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        286..304
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        332..558
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             559..739
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    786..911
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1404..1637
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1822..1866
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2044..2292
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2044..2064
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2215..2234
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2314..2389
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2445..2562
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2701..3879
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2932..3870
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3827..3847
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3892..4491
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3892..3911)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4388..4408
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4507..5095
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4560..4580)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4978..4989)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5167..5400
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5478..5599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5515..5534)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5569..5588
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5639..5774
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5794..5813)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5795..5813)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5823..5840)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5823..5839)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5832..5854)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5981..6436
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6068..6087)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6278..6299
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6462..6566
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6567..6635,6636..7427)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6785..6804)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7598..8186
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8087..8106
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
       61 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
      121 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      181 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      241 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
      301 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
      361 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
      421 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
      481 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
      541 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      601 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      661 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
      721 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
      781 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
      841 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
      901 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
      961 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     1021 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     1081 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     1141 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     1201 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     1261 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     1321 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     1381 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     1441 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     1501 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     1561 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     1621 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     1681 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     1741 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     1801 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     1861 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     1921 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     1981 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     2041 agagagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     2101 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     2161 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     2221 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     2281 ggacgaaaca ccgaacagag agtatccaac acagttttag agctagaaat agcaagttaa
     2341 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttaagct
     2401 tggcgtaact agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2461 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2521 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2581 agcagagatc cactttggcg ccggctcgag ggggcccggg tgcaaagatg gataaagttt
     2641 taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt
     2701 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     2761 ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     2821 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     2881 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     2941 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     3001 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     3061 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     3121 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     3181 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     3241 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     3301 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3361 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3421 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3481 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3541 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3601 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3661 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3721 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3781 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3841 gtggttcaaa gtttttttct tccatttcag gtgtcgtgac gtacggccac catgaccgag
     3901 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     3961 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     4021 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     4081 gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa
     4141 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     4201 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     4261 ttcctggcca ccgtcggagt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     4321 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     4381 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     4441 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aacgcgttaa
     4501 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4561 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4621 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4681 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4741 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4801 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4861 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4921 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4981 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5041 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     5101 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5161 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     5221 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5281 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5341 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5401 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5461 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5521 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5581 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     5641 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5701 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     5761 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     5821 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5881 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5941 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     6001 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6061 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6121 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6181 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6241 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6301 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6361 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6421 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6481 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6541 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6601 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6661 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6721 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6781 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6841 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6901 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6961 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7021 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7081 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7141 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7201 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7261 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7321 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7381 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7441 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7501 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7561 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7621 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7681 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7741 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7801 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7861 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7921 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7981 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8041 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8101 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8161 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8221 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8281 accgtattac cgcctttgag tgagctgata ccgctcgccg cag
//
