LOCUS       sequence-398468-       10299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Based on CROP-seq-opti (addgene #106280), we replaced the PURO 
            resistance gene with a dsRed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10299)
  TITLE     CROP-seq-opti-dsRed
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CROP-seq-opti-dsRed
FEATURES             Location/Qualifiers
     source          1..10299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      420..1008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     909..928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1162..1179
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1296..1317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1332..1337,1338..1355,1356..1362)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1370..1386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1375..1397
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1394..1410
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1394..1410
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1429..1449
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1431..1449
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1477..1703
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1704..1884
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1931..2056
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2549..2782
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2967..3011
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3192..3309
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3448..4626
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3679..4617
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4574..4594
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4633..4642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4639..5313
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(4819..4839)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5037..5056
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5231..5254
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5329..5917
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5382..5402)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5800..5811)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6073..6321
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6073..6093
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6244..6263
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             8341..8521
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8599..8720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8636..8655)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8690..8709
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8760..8895
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8915..8934)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8916..8934)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8944..8961)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8944..8960)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8953..8975)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9102..9557
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9189..9208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9399..9420
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9583..9687
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9688..9756,9757..10299,1..249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9906..9925)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
       61 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
      121 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
      181 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
      241 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      301 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
      361 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
      421 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      481 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      541 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      601 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      661 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      721 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      781 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      841 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      901 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      961 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1021 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1081 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     1141 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     1201 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1261 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     1321 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     1381 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     1441 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     1501 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     1561 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     1621 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     1681 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     1741 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     1801 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     1861 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     1921 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     1981 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     2041 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     2101 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     2161 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     2221 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     2281 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     2341 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     2401 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     2461 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     2521 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     2581 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     2641 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     2701 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     2761 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     2821 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     2881 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     2941 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     3001 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     3061 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     3121 atagagttag gcagggatat tcaccattat cgtttcagaa tcttgagaca aatggcagta
     3181 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3241 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3301 caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg
     3361 gcccgggtgc aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc
     3421 ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca
     3481 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga
     3541 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     3601 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     3661 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
     3721 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
     3781 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     3841 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     3901 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     3961 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     4021 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     4081 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     4141 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     4201 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     4261 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     4321 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     4381 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     4441 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     4501 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     4561 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     4621 tcgtgacgta cggccaccat ggatagcact gagaacgtca tcaagccctt catgcgcttc
     4681 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     4741 ggcaagccct acgagggcac ccagaccgcc aagctgcagg tgaccaaggg cggccccctg
     4801 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt gtacgtgaag
     4861 caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag
     4921 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     4981 gacggcacct tcatctacca cgtgaagttc atcggcgtga acttcccctc cgacggcccc
     5041 gtaatgcaga agaagactct gggctgggag ccctccaccg agcgcctgta cccccgcgac
     5101 ggcgtgctga agggcgagat ccacaaggcg ctgaagctga agggcggcgg ccactacctg
     5161 gtggagttca agtcaatcta catggccaag aagcccgtga agctgcccgg ctactactac
     5221 gtggactcca agctggacat cacctcccac aacgaggact acaccgtggt ggagcagtac
     5281 gagcgcgccg aggcccgcca ccacctgttc cagacgcgtt aagtcgacaa tcaacctctg
     5341 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5401 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5461 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5521 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5581 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5641 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5701 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     5761 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5821 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5881 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     5941 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6001 ttcactccca acgaagacaa gatttcacca ttatcgtttc agacccacct cccaaccccg
     6061 aggggaccca gagagggcct atttcccatg attccttcat atttgcatat acgatacaag
     6121 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
     6181 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     6241 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     6301 ttgtggaaag gacgaaacac cggagacggt tgtaaatgag cacacaaaat acacatgcta
     6361 aaatattata ttctatgacc tttataaaat caaccaaaat cttcttttta ataactttag
     6421 tatcaataat tagaattttt atgttccttt ttgcaaactt ttaataaaaa tgagcaaaat
     6481 aaaaaaacgc tagttttagt aactcgcgtt gttttcttca cctttaataa tagctactcc
     6541 accacttgtt cctaagcggt cagctcctgc ttcaatcatt ttttgagcat cttcaaatgt
     6601 tctaactcca ccagctgctt taactaaagc attgtcttta acaactgact tcattagttt
     6661 aacatcttca aatgttgcac ctgattttga aaatcctgtt gatgttttaa caaattctaa
     6721 tccagcttca acagctattt cacaagcttt catgatttct tcttttgtta ataaacaatt
     6781 ttccataata catttaacaa catgtgatcc agctgctttt tttacagctt tcatgtcttc
     6841 taaaactaat tcataatttt tgtcttttaa tgcaccaata tttaatacca tatcaatttc
     6901 tgttgcacca tctttaattg cttcagaaac ttcgaatgct tttgtagctg ttgtgcatgc
     6961 acctagagga aaacctacaa catttgttat tcctacattt gtgcctttta ataattcttt
     7021 acaatagctt gttcaatatg aattaacaca aactgttgca aaatcaaatt caattgcttc
     7081 atcacataat tgtttaattt cagctttcgt agcatcttgt tttaataatg tgtgatctat
     7141 atatttgttt agtttcattt tttctcctat atattcattt ttaattttaa ttctttaata
     7201 atttcgtcta ctttaacttt agcgttttga acagattcac caacacctat aaaataaatt
     7261 tttagtttag gttcagttcc acttgggcga acagcaaatc atgacttatc ttctaaataa
     7321 aattttagta agtcttgtcc tggcatatta tacattccat cgatgtagtc ttcaacatta
     7381 acaactttaa gtccagcaat ttgagttaag ggtgttgctc tcaatgattt cattaatggt
     7441 tcaattttta atttcttttc ttctggttta aaattcaagt ttaaagtgaa agtgtaatat
     7501 gcacccattt ctttaaataa atcttctaaa tagtctacta atgttttatt ttgtttttta
     7561 taaaatcaag cagcctctgc tattaatata gaagcttgta ttccatcttt atctctagct
     7621 gagtcatcaa ttacatatcc ataactttct tcataagcaa aaacaaaatt taatccgtta
     7681 tcttcttctt tagcaatttc tctacccatt catttaaatc cagttaaagt ttttacaata
     7741 ttaactccat atttttcatg agcgattcta tcacccaaat cacttgttac aaaacttgaa
     7801 tatagagccg gattttttgg aatgctattt aagcgtttta gatttgataa ttttcaatca
     7861 attaaaattg gtcctgtttg atttccatct aatcttacaa aatgaccatc atgttttatt
     7921 gccattccaa atctgtcagc atctgggtca ttcataataa taatatctgc atcatgttta
     7981 ataccatatt caagcggtat ttttcatgca ggatcaaatt ctggatttgg atttacaaca
     8041 tttttaaatg tttcatcttc aaatgcatgc tcttcaacct caataacgtt atatcctgat
     8101 tcacgtaata tttttggggt aaatttagtt cctgttccat taactgcgct aaaaataatt
     8161 tttaaatctt ttttagcttc ttgctctttt ttgtacgtct ctgtttaaga gctatgctgg
     8221 aaacagcata gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
     8281 gtcggtgctt ttttaagctt ggcgtaacta gatcttgaga cactgctttt tgcttgtact
     8341 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8401 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8461 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8521 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     8581 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8641 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8701 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8761 tcccgcccct aactccgccc agttccgcct attctccgcc ccatggctga ctaatttttt
     8821 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8881 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     8941 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     9001 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     9061 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     9121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     9181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     9241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     9301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     9361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     9421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     9481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     9541 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9601 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9661 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     9721 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     9781 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     9841 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     9901 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     9961 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
    10021 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
    10081 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
    10141 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
    10201 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
    10261 ttaactggcg aactacttac tctagcttcc cggcaacaa
//
