LOCUS       sequence-399198-        9761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses codon optimized thermostabile TccCas13a in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Mahas A, Marsic T, Lopez-Portillo Masson M, Wang Q, Aman R, Zheng C,
            Ali Z, Alsanea M, Al-Qahtani A, Ghanem B, Alhamlan F, Mahfouz M
  TITLE     Characterization of a thermostable Cas13 enzyme for one-pot 
            detection of SARS-CoV-2.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Jul 12;119(28):e2118260119. doi: 
            10.1073/pnas.2118260119. Epub 2022 Jun 28.
  PUBMED    35763567
REFERENCE   2  (bases 1 to 9761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-07-12"; volume: "119(28):e2118260119. 
            doi"; pages: " 10.1073/pnas.2118260119. Epub 2022 Jun 28"
COMMENT     Sequence Label: TccCas13a Twin SUMO
FEATURES             Location/Qualifiers
     source          1..9761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      77..665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     566..585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     819..836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    851..990
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(916..935)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1076..1098
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1092..1283)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2595..3677)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3639..3658
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3678..3755)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3961..3980
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4064..4083
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4064..4082
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4083..4107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4122..4144
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4163..4180
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4190..4207
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(4220..4243,4244..4279,4280..4303)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             4304..4597
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(8325..8343)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8339..8386
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(8561..8566,8567..8583,8584..8589))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8616..8634)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8702..8806
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8807..8875,8876..9667)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9025..9044)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      781 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      841 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      901 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
      961 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     1021 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     1081 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
     1141 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
     1201 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     1261 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
     1321 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
     1381 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
     1441 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
     1501 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
     1561 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc
     1621 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
     1681 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
     1741 cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg
     1801 gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag
     1861 aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca
     1921 ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt
     1981 atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa
     2041 tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc
     2101 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
     2161 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
     2221 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
     2281 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
     2341 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
     2401 cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga
     2461 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt
     2521 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat
     2581 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     2641 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
     2701 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
     2761 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
     2821 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc
     2881 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
     2941 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
     3001 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
     3061 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
     3121 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
     3181 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
     3241 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
     3301 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag
     3361 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
     3421 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
     3481 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
     3541 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
     3601 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
     3661 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc
     3721 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg
     3781 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
     3841 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     3901 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     3961 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     4021 gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata
     4081 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
     4141 gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg
     4201 cggcagccat atggctagct ggagccatcc gcagtttgaa aaaggtggtg gtagcggtgg
     4261 tggttcaggt ggtagtgcat ggtcacaccc tcagtttgag aaaatgtcgg actcagaagt
     4321 caatcaagaa gctaagccag aggtcaagcc agaagtcaag cctgagactc acatcaattt
     4381 aaaggtgtcc gatggatctt cagagatctt cttcaagatc aaaaagacca ctcctttaag
     4441 aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa atggactcct taagattctt
     4501 gtacgacggt attagaatcc aagctgatca gacccctgaa gatttggaca tggaggataa
     4561 cgatattatt gaggctcaca gagaacagat tggtggatcc atgaagatta ccaaacgcaa
     4621 gtggggtgaa caccatccgc cgctgtattt ctaccgtgat gaagatagcg gtcgtctgct
     4681 ggcgcaaaac gaccgtaagc aagattacac cgacaccctg ttcaacgaca tcgcgcagga
     4741 cacctttgaa cgtagcctgc gtaaccgtct gctgaaaacc ccggagaagg gcgacaaacg
     4801 tttctatagc aacgaaatcg tgaaactggt tgagaagctg tgccagggtg cggatgttgc
     4861 ggagattatg aagagcatgg aacgtaacga gaaactgcgt ccgaagaacg agaaagaaat
     4921 taagaacctg aagaaacagc tggacggcac cctgagcgag tacggcaagc gttataccgc
     4981 gccggaaggt gcgatgaccc tgaacgatgc gctgttttac ctggttgagg gcaacccgct
     5041 gaaacaggcg atggcgaagg cggaactggg taaaatccgt gaggcgctga ttaaggagaa
     5101 agaaaaccgt atcaaccgtg tgcgttatag cattaaaaac aacaagatcc cgctgcgtat
     5161 tcaggaagat ggtggcatca ccccgaacaa cgaccgtgcg gcgtggctgc tgggcctgat
     5221 gaagccggcg gacccggcga aaggcattac cgactgctac ccgctgctgg gtgaactgga
     5281 ggaagttttc gactttgata aactgagcaa gaccctgcac gagaaaatca gccgttgcca
     5341 aggtcgtccg cgtagcattg cgatggcggt ggacgaggcg ctgaaacagt acctgcgtga
     5401 actgtgggag aagagcccga gccgtcagca agacctgaaa tactatttcc aagcggttca
     5461 ggaatatttc aaggataact ttccgatccg taccaaacgt atgggcgcgc gtctgcgtca
     5521 ggaactgctg aaggacaaaa ccagcctgag ccgtctgctg gagccgaagc acatggcgaa
     5581 cgcggtgcgt cgtcgtctga tcaaccaaag cacccagatg cacattctgt acggtaaact
     5641 gtacgcgtat tgctgcggcg aggatggccg tctgctggtt aacagcgaaa ccctgcaacg
     5701 tatccaggtg cacgaggcgg ttaagaaaca agcgatgacc gcggtgctgt ggagcattag
     5761 ccgtctgcgt tacttctatc agtttgagga cggcgacatc ctgagcaaca aaaacccgat
     5821 taaggatttc cgtgacaagt ttctgcgtga taccaacaaa tacacccacg aagacgttga
     5881 ggcgtgcaag gaaaaactgc aagatttctt tccgctgaag gaactgcagg agaagatcaa
     5941 agaggatgcg aagggcctgc aagaaaccga caacaaacag gcggatacca ccgacttcaa
     6001 ggcgatcggt cacattgttc gtgacgatcg taaactgtgc aaccaactgc tggcggagtg
     6061 cgtgagctgc atcggtgaac tgcgtcacca catttttcac tacaagaacg tgaccctgat
     6121 ccaggcgctg aaacgtattg cggataaggt gaaaccggag gacctgagcg ttctgcgtgc
     6181 gatctatctg ctggaccgtc gtaacctgaa gaaagcgttc gcgaagcgta ttagcagcat
     6241 gaacctgccg ctgtactatc gtgaggacct gctgagccgt atctttaaga aagagggcac
     6301 cgcgttcttt ctgtacagcg cgaaaattca aatgaccccg agcttccagc gtgtttatga
     6361 acgtggcaag aacctgcgtc gtgagtttga atgcgagcgt atgaaagcgg aggcgagcaa
     6421 cggtcaaaac ggccaggacg gtgatcgtct gaagtggttt cgtcagctgg cggcgggtga
     6481 tagcgcggac acccacttta actgggcggt tgaagcgtac gcggagagcg cggcggatgt
     6541 ggaaaacaac gttgagttcg ataccgatgt ggacgcgcag cgtgcgctgc gtaacctgct
     6601 gctgctgatc tatcgtcacc actttctgcc ggaagtgcaa aaggatgaaa ccctggttac
     6661 cggcaaaatt cacaaggtgc tggagcgtaa ccgtcagctg agcgaaggtc aaggcccgaa
     6721 ccagggcaaa gcgcacggtt acagcgtgat cgaggaactg tatcacgagg gtatgccgct
     6781 gagcgacctg atgaagcaac tgcagcgtcg tattagcgaa accgagcgtg aaagccgtga
     6841 actggcgcaa gagaaaaccg attacgcgca gcgtttcatc ctggacattt ttgcggaggc
     6901 gttcaacgat tttctggaag cgcactacgg cgaggaatat ctggaaatca tgagcccgcg
     6961 taaagacgcg gaggcggcga agaaatgggt gaaagaaagc aagaccgttg atctgaagac
     7021 cagcattgac gagaaagaac cggagggtca cctgctggtg ctgtacccgg ttctgcgtct
     7081 gctggatgaa cgtgagctgg gtgaactgca gcaacagatg atccgttatc gtaccagcct
     7141 ggcgagctgg caaggcgaga gcaacttcag cgaggaaatc cgtattgcgg gtcagattga
     7201 ggaactgacc gaactggtta agctgaccga accggagccg caatttgcgg aggaagtgtg
     7261 gggcaaacgt gcgaaggaag cgttcgagga ctttatcgag ggtaacatga aaaactacga
     7321 agcgttctat ctgcagagcg ataacaacac cccggtttac cgtcgtaaca tgagccgtct
     7381 gctgcgtagc ggcctgatgg gtgtgtacca aaaggttctg gcgagccaca aacaggcgct
     7441 gaagcgtgac tatctgctgt ggagcgagaa gcactggaac gtgaaagatg aaaacggcgc
     7501 ggacatcagc agcgcggaac aagcgcagtg cctgctgcaa cgtctgcacc gtaagtacgc
     7561 ggagagcccg agccgtttca ccgaggaaga ctgcaaactg tacgagaagg ttctgcgtcg
     7621 tctggaagat tataaccagg cggtgaaaaa cctgagcttt agcagcctgt acgaaatctg
     7681 cgtgctgaac ctggaaattc tgagccgttg ggtgggtttc gttcaagatt gggagcgtga
     7741 catgtatttt ctgctgctgg cgtgggttcg tcagggcaag ctggacggta tcaaagagga
     7801 agatgtgcgt gacattttca gcgaaggcaa catcattcgt aacctggttg ataccctgaa
     7861 aggtgaaaac atgaacgcgt tcgagagcgt gtactttccg gaaaacaaag gcagcaagta
     7921 tctgggtgtg cgtaacgatg ttgcgcacct ggacctgatg cgtaagaacg gctggcgtct
     7981 ggaagcgggt aaaacctgca gcgttatgga ggactacatc aaccgtctgc gtttcctgct
     8041 gagctatgat cagaagcgta tgaacgcggt gaccaaaacc ctgcaacaga ttttcgaccg
     8101 tcacaaggtg aaaattcgtt ttaccgttga gaagggtggc atgctgaaaa tcgaagatgt
     8161 gaccgcggac aagattgttc acctgaaagg cagccgtctg agcggtatcg agattccgag
     8221 ccacggcgag cgttttattg acaccctgaa ggcgctgatg gtttacccgc gtggttaagc
     8281 ggccgcactc gaggcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     8341 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
     8401 tatatccgga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca
     8461 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt
     8521 gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata
     8581 agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     8641 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     8701 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8761 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8821 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8881 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8941 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9001 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     9061 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9121 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9181 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9241 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9301 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
     9361 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9421 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9481 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9541 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9601 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9661 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9721 ttaatttaaa aggatctagg tgaagatcct ttttgataat c
//
