LOCUS       sequence-399322-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RHOA protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  TITLE     TREAT-AD plasmids Jun 2023
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RHOAA-c035
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             637..681
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg ctgcaattag gaagaaacta gtaatagtgg gcgacggcgc gtgtggtaag
      121 acctgcttgc tgattgtttt ctcgaaagat cagtttccgg aggtctatgt gcctacggtt
      181 ttcgagaact acgtggcgga tatcgaagtc gatggtaaac aggttgaact ggcactgtgg
      241 gataccgcgg gtcaggagga ctacgaccgt ctgcgtccgc tgtcctaccc ggacaccgat
      301 gttattctga tgtgctttag cattgatagc ccagacagct tggaaaacat cccggagaaa
      361 tggacgccgg aggttaaaca cttctgcccg aatgtgccga tcatcctcgt aggtaacaag
      421 aaggacctgc gcaacgatga acatactaga cgtgagctgg cgaagatgaa acaagaaccg
      481 gttaagccgg aggaaggccg tgacatggcg aatcgtattg gtgcctttgg ttatatggaa
      541 tgttctgcta agaccaaaga cggcgttcgc gaggtgttcg aaatggctac ccgtgcggca
      601 ttacaagccc gtcgcggtag cagtaaaggt ggatacggcc tgaatgatat ctttgaagcg
      661 cagaagattg aatggcatga atga
//
